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A polygalacturonase gene OsPG1 modulates water homeostasis in rice

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The Crop Journal 2024年第1期12卷 79-91页

作者： Qinwen Zou Ranran Tu Jiajun Wu Tingting Huang Zhihao Sun Zheyan Ruan Hongyu Cao Shihui Yang Xihong Shen Guanghua he Hong Wang Rice Research institute Key Laboratory of Application and Safety Control of Genetically Modified CropsAcademy of Agricultural SciencesSouthwest UniversityChongqing 400715China State Key Laboratory of Rice Biology and Breeding Key Laboratory for Zhejiang Super Rice ResearchChina National Center for Rice ImprovementChina National Rice Research InstituteHangzhou 311401ZhejiangChina

A dynamic plant architecture is the basis of plant adaptation to changing *** many genes regulating leaf rolling have been identified,genes directly associated with water homeostasis are largely ***,we isolated a rice mutant,dynamic leaf rolling 1(dlr1),characterized by‘leaf unfolding in the morning-leaf rolling at noon-leaf unfolding in the evening’during a sunny *** content was decreased in rolled leaves and water sprayed on leaves caused reopening,indicating that in vivo water deficiency induced the leaf ***-based cloning and expression tests demonstrated that an A1400G single base mutation in Oryza sativa Polygalacturonase 1(OsPG1)/PHOTO-SENSITIVE LEAF ROLLING 1(PSL1)was responsible for the dynamic leaf rolling phenotype in the dlr1 ***1 encodes a polygalacturonase,one of the main enzymes that degrade demethylesterified homogalacturonans in plant cell ***1 was constitutively expressed in various tissues and was enriched in *** of the OsPG1 gene exhibited defects in stomatal closure and decreased stomatal density,leading to reduced transpiration and excessive water loss under specific conditions,but had normal root *** analysis revealed that mutation of OsPG1 led to reduced pectinase activity in the leaves and increased demethylesterified homogalacturonans in guard *** findings reveal a mechanism by which OsPG1 modulates water homeostasis to control dynamic leaf rolling,providing insights for plants to adapt to environmental variation.

关键词： Photosynthesis Stomata Transpiration Leaf rolling

High-throughput screening system of citrus bacterial cankerassociated transcription factors and its application to the regulation of citrus canker resistance

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Journal of Integrative Agriculture 2024年第1期23卷 155-165页

作者： Jia Fu Jie Fan Chenxi Zhang Yongyao Fu Baohang Xian Qiyuan Yu Xin Huang Wen Yang Shanchun Chen Yongrui he Qiang Li Integrative Science Center of Germplasm Creation in Western china(Chongqing)Science City Citrus Research InstituteSouthwest UniversityChongqing 400712China School of Advanced Agriculture and Bioengineering Yangtze Normal UniversityChongqing 408100China National Citrus Engineering Research Center Chongqing 400712China

One of the main diseases that adversely impacts the global citrus industry is citrus bacterial canker(CBC),caused by the bacteria Xanthomonas citri ***(Xcc).Response to CBC is a complex process,with both proteinDNA as well as protein–protein interactions for the regulatory *** detect such interactions in CBC resistant regulation,a citrus high-throughput screening system with 203 CBC-inducible transcription factors(TFs),were *** the upstream regulators of target by yeast-one hybrid(Y1H)methods was also performed.A regulatory module of CBC resistance was identified based on this *** TF(CsDOF5.8)was explored due to its interactions with the 1-kb promoter fragment of CsPrx25,a resistant gene of CBC involved in reactive oxygen species(ROS)homeostasis *** mobility shift assay(EMSA),dual-LUC assays,as well as transient overexpression of CsDOF5.8,further validated the interactions and transcriptional *** CsDOF5.8–CsPrx25 promoter interaction revealed a complex pathway that governs the regulation of CBC resistance via *** high-throughput Y1H/Y2H screening system could be an efficient tool for studying regulatory pathways or network of CBC resistance *** addition,it could highlight the potential of these candidate genes as targets for efforts to breed CBC-resistant citrus varieties.

关键词： citrus bacterial canker(CBC) high-throughput screening system transcription factor(TF) yeast-one hybrid(Y1H) CsPrx25

Mitochondrial cytochrome b sequence variations and phylogeny of the East Asian bagrid catfishes

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Progress in Natural Science:Materials International 2002年第6期 23-27页

作者： PENG Zuogang, he Shunping and ZHANG Yaoguang(1. institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, china 2. School of Life Science, Southwest china normal university, Chongqing 400715, china) institute of Hydrobiology，Chinese Academy of Sciences，Wuhan 430072，china School of Life Science，Southwest china normal university，Chongqing 400715，china institute of Hydrobiology，Chinese Academy of Sciences，Wuhan 430072，china School of Life Science，Southwest china normal university，Chongqing 400715，china

The mitochondrial DNA cytochrome b gene was sequenced from 8 bagrid catfishes in china. Aligned with cytochrome b sequences from 9 bagrid catfishes in Japan, Korea and Russia retrieved from GenBank, and selected Silurus meridionalis, Liobagrus anguillicauda, Liobagrus reini and Phenacogrammus interruptus as outgroups, we constructed a matrix of 21 DNA sequences. The Kimura's two-parameter distances were calculated and molecular phylogenetic trees were constructed by using the maximum parsimony (MP) and neighbor-joining (NJ) methods. The results show that (i) there exist 3-bp deletions of mitochondrial cytochrome 6 gene compared with cypriniforms and characiforms; (ii) the molecular phylogenetic tree suggests that bagrid catfishes form a monophyletic group, and the genus Mystus is the earliest divergent in the East Asian bagrid catfishes, as well as the genus Pseudobagrus is a monophyletic group but the genus Pelteobagrus and Leiocassis are complicated; and (iii) the evolution rate of the East Asian bagr

关键词： cytochrome b gene molecular phylogeny Bagridae Siluriformes.

Arbuscular mycorrhiza and plant growth promoting endophytes facilitates accumulation of saponin under moderate drought stress

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Chinese herbal Medicines 2024年第2期16卷 214-226页

作者： Shubiao Qian Qing Zhang Sha Li Rui Shi Xiahong he Shuhui Zi Tao Liu Yunnan Agricultural university Kunming 650201China National&Local Joint Engineering Research Center on Gemplasm Utilization&Innovation of Chinese Medicinal Materials in Southwest china Yunnan Agricultural UniversityKunming 650201China institute of Sericulture and Honeybee Honghe 661101China Southwest Forestry university Kunming 650201China

Objective: Paris polyphylla var. yunnanensis, one of the important medicinal plant resources in Yunnan,china, usually takes 6–8 years to be harvested. Therefore, it is urgent to find a method that can not only shorten its growth years, but also improve its quality. In this study, we examined the effects of a combination treatment of arbuscular mycorrhizal fungi(AMF) and plant growth-promoting endophytes(PGPE)and drought stress on the accumulation of saponins in ***: P. polyphylla var. yunnanensis was infected with a mixture of AMF and PGPE under drought stress. The content of saponins, as well as morphological, physiological, and biochemical indicators, were all measured. The UGTs gene related to saponin synthesis was obtained from transcriptome data by homologous comparison, which were used for RT-PCR and phylogenetic ***: Regardless of water, AMF treatment could infect the roots of P. polyphylla var. yunnanensis, however double inoculation with AMF and PGPE(AMF + PGPE) would reduce the infection rate of AMF. Plant height, aboveground and underground fresh weight did not differ significantly between the single inoculation AMF and the double inoculation treatment under different water conditions, but the inoculation treatment significantly increased the plant height of P. polyphylla var. yunnanensis compared to the noninoculation treatment. Single inoculation with AMF considerably increased the net photosynthetic rate,stomatal conductance, and transpiration rate of P. polyphylla var. yunnanensis leaves under various water conditions, but double inoculation with AMF + PGPE greatly increased the intercellular CO2concentration and chlorophyll fluorescence parameter(Fv/Fm). Under diverse water treatments, single inoculation AMF had the highest proline content, whereas double inoculation AMF + PGPE may greatly improve the amount of abscisic acid(ABA) and indoleacetic acid(IAA) compared to normal water under moderate drought. Double inoculation AMF + P

关键词： arbuscular mycorrhizal fungi gene expression Paris polyphylla Smith var.yunnanensis (Franch.)Hand.-Mazz plant growth-promoting endophytes steroidal saponin

Expression of Human Papillomavirus Type 16 L1 Protein in Transgenic Tobacco Plants

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Acta Biochimica et Biophysica Sinica 2005年第3期37卷 153-158页

作者： Hong-Li LIU Wen-Sheng LI Ting LEI Jing ZheNG Zheng ZHANG Xiao-Fei YAN Zhe-Zhi WANG Yi-Li WANG L-Sheng SI institute of Cancer Research,School of Life Science & technology,Xi'an Jiaotong university.Xi'an 710061,china First Hospital of Xi'an Jiaotong university Xi'an 710061,china Shaanxi normal university,Xi'an 710062china instituteofCancerResearch SchoolofLifeScience&TechnologyXi'anJiaotongUniversityXi'an710061China

To develop a plant expression system for the production of the human papillomavirus type 16(HPV16)vaccine,we investigated whether the HPV16 L1 protein can be expressed in tobacco plants andwhether it can be used as the cheapest form of edible *** HPV16 L1 coding sequence was amplifiedby PCR using specific primers from the plasmid pGEM-T-HPV16 containing the template sequence,andsubcloned into the intermediate vector pUCmT and binary vector pBI121 consecutively to obtain the plantexpression plasmid *** T-DNA regions of the pBI-L1 binary vector contained the constitutiveCauliflower mosaic virus(CaMV)35S promoter and the neomycin phosphotransferase npt Ⅱ gene,whichallowed the selection of transformed plants using *** tobacco plants were transformed by co-cultivating them,using the leaf disc method,with Agrobacterium tumefaciens LBA4404,which harbored theplant expression *** regenerated transgenic tobacco plants were selected using kanamycin,andconfirmed by *** results of the Southern blot assay also showed that the HPV16 L1 gene was inte-grated stably into the genome of the transformed tobacco *** Western blot analysis showed that thetransformed tobacco leaves could express the HPV16 L1 ***,it was demonstrated byELISA assay that the expressed protein accounted for 0.034%-0.076% of the total soluble leaf protein,wasable to form 55 nm virus-like particles compatible with HPV virus-like particle(VLP),and induced mouseerythrocyte hemagglutination in *** present results indicate that the HPV16 L1 protein can be ex-pressed in transgenic tobacco plants and the expressed protein possesses the natural features of the HPV16L1 protein,implying that the HPV16 L1 transgenic plants can be potentially used as an edible vaccine.

关键词： human papillomavirus(HPV) virus-like particle transgenic tobacco plant vaccine Agrobacterium tumefaciens Ll major capsid protein

Caveolin-1 gene silencing promotes the activation of PI3K/AKT dependent on Erα36 and the transformation of MCF10A^(CE)

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Science china(Life Sciences) 2010年第5期53卷 598-605页

作者： FENG Shuang1,WANG Yang1,WANG Xi1,WANG ZhaoYi3,CUI YuYing1,LIU Jing4,ZHAO ChunHui1,JIN Mei1,2 & ZOU Wei1,2 1 College of Life Science,Liaoning normal university,Dalian 116029,china 2 Liaoning Key Laboratory of Biotechnology and Molecular Drug R&D,Dalian 116029,china 3 Cancer Center,Creighton university,2500 California Plaza,Omaha,NE 68178,USA 4 First Clinical Hospital of Dalian Medical university,Dalian 116011,china 1. College of Life Science Liaoning Normal University Dalian 116029 China3. Cancer Center Creighton University 2500 California Plaza Omaha NE 68178 USA4. First Clinical Hospital of Dalian Medical University Dalian 116011 China2. Liaoning Key Laboratory of Biotechnology and Molecular Drug R&D Dalian 116029 China

ERα36,a variant of estrogen receptor-α,acts as a dominant-negative factor in both estrogen-dependent and estrogen-independent transactivation signaling pathways,and is a key factor in the promotion,progression and prognosis of breast ***-1,a 22-to 24-kD integral membrane protein,may function as a tumor suppressor in inhibiting of many growth-promoting signaling *** was shown that downregulation of Caveolin-1 strengthens the interaction of ERα and *** conclusion,Caveolin-1 gene silencing activated the PI3K/AKT signaling pathway in an ERα36-dependent *** finding may provide a promising therapeutic target of breast cancer.

关键词： Caveolin-1 Estrogen receptor PI3K/AKT

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转铁蛋白受体2及其功能与相关疾病

生物化学与生物物理进展 2003年第4期30卷 533-536页

作者：常彦忠段相林钱忠明香港理工大学应用生物及化学科技学系铁代谢实验室

转铁蛋白受体 2 (transferrinreceptor 2 ,TfR2 )是最近发现的一种重要铁代谢蛋白 .研究显示它不仅是一种介导肝脏细胞铁摄取的主要蛋白 ,而且在调节小肠铁吸收方面起着极其关键的作用 ,是控制肝脏铁调素合成和释放的关键成分 .已经证实... 详细信息

转铁蛋白受体 2 (transferrinreceptor 2 ,TfR2 )是最近发现的一种重要铁代谢蛋白 .研究显示它不仅是一种介导肝脏细胞铁摄取的主要蛋白 ,而且在调节小肠铁吸收方面起着极其关键的作用 ,是控制肝脏铁调素合成和释放的关键成分 .已经证实 ,TfR2基因突变是遗传性血色素沉着病的重要原因之一 .

关键词：转铁蛋白受体2 功能铁吸收铁代谢遗传性血色素沉着病

The effect of C-terminal fragment of JNK2 on the stability of p53 and cell proliferation

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Cell Research 2004年第5期14卷 434-438页

作者： Zhi Min YIN1,*, Jian SIMA1, Yi Fan WU1, Jian ZHU1, Yong JIANG2 1Jiangsu Province Key Laboratory of Biochemistry and Molecular Biology, College of Life Science, Nanjing normal university, Nanjing 210097, china 2Department of Pathophysiology and Key Lab for Shock and Microcirculation of PLA, The First Military Medical university, Guangzhou 510515, china Jiangsu Province Key Laboratory of Biochemistry and Molecular Biology College of Life Science Nanjing Normal University Nanjing 210097 China. Zhiminy_2000@***

The basal activity of JNK is low in normal growing cells and inactivated JNK targets p53 for ubiquitination. To elucidate if the C-terminal part of JNK is responsible for its binding to p53, the low background tet-off inducible NIH3T3 cell line was selected by luciferase reporter gene and a double stable C-JNK Aa (203-424) cell line was established. After withdrawing tetracycline, the C-JNK fragment expression was induced and cell growth was dramati- cally inhibited 24 h later. However, the expresion of p53 was found to be increased after the induction of C-JNK fragment, evaluated by transfecting p21waf-luciferase reporter genes. Our further studies showed that C-JNK fragment could form complex with p53 both in vivo and in vitro. Induction of C-JNK fragment in vivo can increase p53 stability by inhibiting p53 ubiquitination.

关键词： tet-off expression system c-Jun N-terminal kinase p53 cell proliferation.

Iron metabolism in the mononuclear phagocyte system

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Progress in Natural Science:Materials International 2008年第10期18卷 1197-1202页

作者： Weina Kong Xianglin Duan Zhenhua Shi Yanzhong Chang The Key Laboratory of Animal Physiology,Biochemistry and Molecular Biology of hebei Province,hebei normal university,Shijiazhuang 050016,china The Key Laboratory of Animal Physiology Biochemistry and Molecular Biology of Hebei Province Hebei Normal University Shijiazhuang 050016 China

The maintenance of body iron homeostasis requires the coordination of multiple regulatory mechanisms of iron *** mononuclear phagocyte system (MPS,composed of monocytes,macrophages,and their precursor cells) is crucial in the maintenance of iron *** of iron is carried out by specialized macrophages via engulfment of aged *** iron stores of macrophages depend on the levels of recovered and exported ***,the molecular mechanisms underlying iron homeostasis in macrophages are poorly *** studies characterizing the function and regulation of natural resistance-associated mac- rophage protein I (Nrampl),divalent metal transporter 1 (DMTI),HLA-linked hemechromatosis gene (HFE),ferroportin 1 (FPNI), and hepcidin are rapidly expanding our knowledge on the molecular level of MPS iron *** studies are deepening our under- standing about the molecular mechanism of iron homeostasis and iron-related diseases.

关键词： Mononuclear phagocyte system CD 163 Natural resistance-associated macrophage protein 1 Divalent metal transporter 1 HFE Ferroportin 1 hepcidin