Arbuscular mycorrhiza and plant growth promoting endophytes facilitates accumulation of saponin under moderate drought stress

作     者：Shubiao Qian Qing Zhang Sha Li Rui Shi Xiahong He Shuhui Zi Tao Liu Shubiao Qian;Qing Zhang;Sha Li;Rui Shi;Xiahong He;Shuhui Zi;Tao Liu

作者机构：Yunnan Agricultural UniversityKunming 650201China National&Local Joint Engineering Research Center on Gemplasm Utilization&Innovation of Chinese Medicinal Materials in Southwest ChinaYunnan Agricultural UniversityKunming 650201China Institute of Sericulture and HoneybeeHonghe 661101China Southwest Forestry UniversityKunming 650201China 

出 版 物：《Chinese Herbal Medicines》 (中草药（英文版）)

年 卷 期：2024年第16卷第2期

页      面：214-226页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1007[医学-药学(可授医学、理学学位)] 10[医学] 

基　　金：supported by grants from Major Special Projects of the Ministry of Science and Technology(No.2021yfd1000202 2021YFD1601003) the Key R&D program of Yunnan Province(No.202103AC100003) National Natural Science Foundation of China(No.31860075) 

主　　题：arbuscular mycorrhizal fungi gene expression Paris polyphylla Smith var.yunnanensis (Franch.)Hand.-Mazz plant growth-promoting endophytes steroidal saponin 

摘      要：Objective: Paris polyphylla var. yunnanensis, one of the important medicinal plant resources in Yunnan,China, usually takes 6–8 years to be harvested. Therefore, it is urgent to find a method that can not only shorten its growth years, but also improve its quality. In this study, we examined the effects of a combination treatment of arbuscular mycorrhizal fungi(AMF) and plant growth-promoting endophytes(PGPE)and drought stress on the accumulation of saponins in ***: P. polyphylla var. yunnanensis was infected with a mixture of AMF and PGPE under drought stress. The content of saponins, as well as morphological, physiological, and biochemical indicators, were all measured. The UGTs gene related to saponin synthesis was obtained from transcriptome data by homologous comparison, which were used for RT-PCR and phylogenetic ***: Regardless of water, AMF treatment could infect the roots of P. polyphylla var. yunnanensis, however double inoculation with AMF and PGPE(AMF + PGPE) would reduce the infection rate of AMF. Plant height, aboveground and underground fresh weight did not differ significantly between the single inoculation AMF and the double inoculation treatment under different water conditions, but the inoculation treatment significantly increased the plant height of P. polyphylla var. yunnanensis compared to the noninoculation treatment. Single inoculation with AMF considerably increased the net photosynthetic rate,stomatal conductance, and transpiration rate of P. polyphylla var. yunnanensis leaves under various water conditions, but double inoculation with AMF + PGPE greatly increased the intercellular CO2concentration and chlorophyll fluorescence parameter(Fv/Fm). Under diverse water treatments, single inoculation AMF had the highest proline content, whereas double inoculation AMF + PGPE may greatly improve the amount of abscisic acid(ABA) and indoleacetic acid(IAA) compared to normal water under moderate drought. Double inoculation AMF + P