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Generation of human/rat xenograft animal model for the study of human donor stem cell behaviors in vivo

World Journal of Gastroenterology 2007年第19期13卷 2707-2716页

作者： Yan sun Dong Xiao Xing-Hua Pan Ruo-Shuang Zhang Guang-Hui Cui Xi-Gu Chen center of Experimental Animals Sun Yat-sen University Guangzhou 510080 Guangdong Province China Institute of Comparative Medicine and center of Experimental Animals Southern Medical University Guangzhou 510515 Guangdong Province China Clinic Experimental Department Kunming General Hospital Chengdu Military District of PLA Kunming 650032 Yunnan Province China

AIM： TO accurately and realistically elucidate human stem cell behaviors in vivo and the fundamental mechanisms controlling human stem cell fates in vivo, which is urgently required in regenerative medicine and treatments for some human diseases, a surrogate human-rat chimera model was developed. METHODS： Human-rat chimeras were achieved by in utero transplanting low-density mononuclear cells from human umbilical cord blood into the fetal rats at 9-11 d of gestation, and subsequently, a variety of methods, including flow cytometry, PCR as well as immunohistochemical assay, were used to test the human donor contribution in the recipients. RESULTS： Of 29 live-born recipients, 19 had the presence of human CD45^＋ cells in peripheral blood （PB） detected by flow cytometry, while PCR analysis on genomic DNA from 11 different adult tissues showed that 14 selected from flow cytometry-positive 19 animals possessed of donor-derived human cell engraftment in multiple tissues （i.e. liver, spleen, thymus, heart, kidney, blood, lung, muscle, gut and skin） examined at the time of tissue collection, as confirmed by detecting human 132- microglobulin expression using immunohistochemistry. Tn this xenogeneic system, the engrafted donor-derived human cells persisted in multiple tissues for at least 6 mo after birth. Moreover, transplanted human donor cells underwent site-specific differentiation into CK18-positive human cells in chimeric liver and CEHS-positive human cells in chimeric spleen and thymus of recipients. CONCLUSION： Taken together, these findings suggest that we successfully developed human-rat chimeras, in which xenogeneic human cells exist up to 6 mo later. This humanized small animal model, which offers an in vivo environment more closely resembling to the situations in human, provides an invaluable and effective approach for in vivo investigating human stem cell behaviors, and further in vivo examining fundamental mechanisms controlling human stem cell fates in the fut

关键词： Human umbilical cord blood-derived cells In utero xenogeneic transplantation Human-rat chimeras Embryonic microenvironment In vivo model

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environmental siblings of black agents of human chromoblastomycosis

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Fungal Diversity 2014年第2期65卷 47-63页

作者： V.A.Vicente M.J.Najafzadeh Jiufeng sun R.R.Gomes D.Robl S.G.Marques C.M.P.S.Azevedo G.S.de Hoog CBS-KNAW Fungal Biodiversity Centre P.O.Box 851673508AD UtrechtThe Netherlands Microbiology Parasitology and Pathology Post-graduation ProgramDepartment of Basic PathologyFederal University of ParanáCuritibaPRBrazil Institute for Biodiversity and Ecosystem Dynamics University of AmsterdamAmsterdamThe Netherlands Peking university Health Science center Research Center for Medical MycologyBeijingChina sun-yat-sen Memorial Hospital Sun Yat-sen UniversityGuangzhouChina Second Medical Military university ShanghaiChina King Abdulassiz university Jeddah JeddahSaudi Arabia Department of Parasitology and Mycology and Cancer Molecular Pathology Research CenterGhaem HospitalSchool of MedicineMashhad University of Medical SciencesMashhadIran guangdong Provincial Institute of Public Health Guangdong Provincial Center for Disease Control and PreventionGuangzhouChina Federal university of Maranhão University Hospital of the Federal University of MaranhãoSão LuísBrazil Cedro Laboratory and university Hospital of the Federal university of Maranhão São LuísBrazil Institute of Biomedical Sciences University of São Paulo(USP)CEP 05508-900 São PauloBrazil

Agents of human chromoblastomycosis,a skin disease almost exclusively caused by members of the order Chaetothyriales,are assumed to be traumatically inoculated into the skin with sharp environmental materials such as plant thorns or wooden splinters carrying the respective *** the supposition that such fungi should have their main habitat in the environment,we investigated the occurrence of black fungi in living areas of patients with *** South America Fonsecaea agents are prevalent as agents of the disease,while also related Cladophialophora species,known from other types of skin infections,are known from the *** environmental isolates were preliminarily selected as possible agents of chromoblastomycosis,based on *** from ITS sequence data isolates were attributed to the genera Cladophialophora,Cyphellophora,Exophiala,Fonsecaea,Phialophora,and Veronaea.A total of 45 fungi morphologically identified as Fonsecaea or Cladophialophora isolated from debris and thorns of living prickly plants in Brazil were processed for taxonomic *** analysis revealed that the isolates indeed belonged to the Chaetothyriales,but only rarely an agent of chromoblastomycosis was concerned;only two strains of *** and one *** were isolated from debris *** remaining isolates belonged to hitherto unknown molecular siblings of *** novel taxa are introduced.

关键词： Black yeasts environmental isolation Chromoblastomycosis Opportunistic fungi Virulence Infection route

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Experimental studies on the Danxia landscape morphogenesis in Mt. Danxiashan, South china

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Journal of Geographical Sciences 2015年第8期25卷 943-966页

作者： ZHU Cheng WU Li ZHU Tongxin HOU Rongfeng HU Zhinong TAN Yan sun Wei JIA Tianjiao PENG Hua School of Geographic and Oceanographic Sciences Nanjing University Nanjing 210023 China College of Territorial resources and Tourism Anhui Normal University Wuhu 241002 Anhui China Department of Geography University of Minnesota-Duluth MN 55812 USA Administrative Committee of Danxiashan Scenic Spot Shaoguan 512300 Guangdong China Nanjing Hydraulic Research Institute Nanjing 210029 China School of Geography and Planning Sun Yat-Sen University Guangzhou 510275 China

The formation of Danxia landscapes is too slow to be observed in our life time and the paleoclimates in which Danxia landscapes developed are significantly different from the present. Thus, this study adopted experimental approaches to examine the lithological and paleoclimatic control on the formation of various landscape morphologies in the Mt. Danxiashan, South china. A total of 122 rock samples were collected from a range of Danxia landscape morphologies such as white spots, small and large through caves, honeycomb caves, horizontal grooves, natural bridges, stone pillars, and squama stones. Analyses of the collected samples were conducted in different kinds of experiments, including uniaxial mechanical strength testing; rock resistance against sulfuric acid erosion, freezing, and thawing; X-ray fluorescence spectrometry（XRF） analysis; inductively coupled plasma mass spectrometry（ICP-MS） analysis; and identification analysis under a polarizing microscope. The results indicated that the formation of the various Danxia landscape morphologies could result from one or more of the following processes： differential erosion due to lithological difference, chemical dissolution and recrystallization, freezing and thawing actions, acid corrosion, weathering, lateral erosion of river flows, and tectonic uplifts. Water erosion in humid monsoon climate and the alternations of intensive freezing and thawing actions in the Quaternary glacial stages and the interglacial stages could have had great influences on the formation of Danxia landscape topographies.

关键词： experimental studies rock properties Danxia landform paleoclimates china

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南海白云-荔湾深水区同扩张期岩浆活动特征及构造意义

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地球科学 2023年第10期48卷 3781-3798页

作者：朱筱曦赵中贤卓海腾庞雄郑金云孙龙涛孙珍中国科学院边缘海与大洋地质重点实验室南海海洋研究所南海生态环境工程创新研究院广东广州510301 南方海洋科学与工程广东省实验室(广州) 广东广州511458 中国科学院大学北京100049 中国-巴基斯坦地球科学研究中心伊斯兰堡45320巴基斯坦中山大学海洋科学学院广东珠海519082 中海石油深海开发有限公司广东深圳518054

南海陆缘深水区发育同裂陷期及同扩张期岩浆,但其时空展布特征、岩浆活动量及活动机制存在较大争议.选取三维反射数据质量较高且覆盖全面的珠江口盆地白云-荔湾深水区,对岩浆活动开展解释和岩浆量的统计工作.在钻井的约束下,综合运用反... 详细信息

南海陆缘深水区发育同裂陷期及同扩张期岩浆,但其时空展布特征、岩浆活动量及活动机制存在较大争议.选取三维反射数据质量较高且覆盖全面的珠江口盆地白云-荔湾深水区,对岩浆活动开展解释和岩浆量的统计工作.在钻井的约束下,综合运用反射特征和接触关系分析以及火山地层学方法,开展了19条高分辨率三维多道反射地震资料解释,在此基础上对白云-荔湾凹陷沉积层中侵入和喷发的岩浆岩开展了系统研究,结果显示:(1)研究区自浅部到深部共识别出100余个火成岩体及与岩浆活动相关构造,含3相11亚相,3相包括火山岩相、侵入相和气液相;(2)岩浆活动划分为33.9~23.0 Ma、23.0~19.1 Ma及19.1~16.0 Ma三个期次,各期岩浆产生量逐期减少;(3)空间上,岩浆活动呈现东南多西北少、由南向北逐渐变新的趋势,且岩浆活动大多分布于周围隆起之上.扩张脊于23.6 Ma时南迁,研究区岩浆活动表现为向北跃迁.推测拉张-扩张早期上涌地幔物质可能受到脊吸作用的影响而导致其上涌头部向洋脊偏转,在扩张脊南迁后,脊吸作用变弱,部分上涌地幔向北回撤,从而导致23 Ma之后岩浆活动向北迁移.扩张结束后岩浆(<17 Ma)碱性程度升高,推测岩浆物质来源变深.

关键词：南海北缘白云-荔湾深水区岩浆活动时空展布岩浆量地球物理构造

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Science and Prediction of Heavy Rainfall over china:Research Progress since the Reform and Opening-Up of New china

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Journal of Meteorological Research 2020年第3期34卷 427-459页

作者： Yali LUO Jisong sun Ying LI Rudi XIA Yu DU Shuai YANG Yuanchun ZHANG Jing CHEN Kan DAI Xueshun SHEN Haoming CHEN Feifan ZHOU Yimin LIU Shenming FU Mengwen WU Tiangui XIAO Yangruixue CHEN Huiqi LI Mingxin LI State Key Laboratory of Severe Weather Chinese Academy of Meteorological SciencesChina Meteorological AdministrationBeijing 100081 Collaborative Innovation center on Forecast and Evaluation of Meteorological Disasters Nanjing University of Information Science&TechnologyNanjing 210044 School of Atmospheric Sciences and Guangdong Province Key Laboratory for Climate Change and Natural Disaster StudiesSun Yat-sen UniversityGuangzhou 519082 Key Laboratory of Cloud–Precipitation Physics and Severe Storms Institute of Atmospheric PhysicsChinese Academy of SciencesBeijing 100029 National Meteorological center China Meteorological AdministrationBeijing 100081 State Key Laboratory of Numerical Modeling for Atmospheric Sciences and Geophysical Fluid Dynamics Institute of Atmospheric PhysicsChinese Academy of SciencesBeijing 100029 Zhejiang Institute of Meteorological Sciences Zhejiang Meteorological BureauHangzhou 310008 School of Atmospheric Sciences Chengdu University of Information TechnologyChengdu 610225 Institute of Heavy Rain China Meteorological AdministrationWuhan 43020510 Institute of Tropical and Marine MeteorologyChina Meteorological Administration Guangzhou 510640 Southern Marine Science and Engineering guangdong Laboratory(Zhuhai) Zhuhai 519082 university of Chinese Academy of Sciences Beijing 100049 CAS center for Excellence in Tibetan Plateau earth Sciences Chinese Academy of Sciences(CAS)Beijing 100101

This paper reviews the major progress on development of the science and prediction of heavy rainfall over china since the beginning of the reform and opening-up of new china(roughly between 1980 and 2019).The progress of research on the physical mechanisms of heavy rainfall over china is summarized from three perspectives:1)the relevant synoptic weather systems,2)heavy rainfall in major sub-regions of china,and 3)heavy rainfall induced by *** development and application of forecasting techniques for heavy rainfall are summarized in terms of numerical weather prediction techniques and objective forecasting *** aided by the rapid progress in meteorological observing technology and substantial improvement in electronic computing,studies of heavy rainfall in china have advanced to investigating the evolution of heavy-rain-producing storms and observational analysis of the cloud microphysical features.A deeper and more systematic understanding of the synoptic systems of importance to the production of heavy rainfall has also been *** forecast of heavy rainfall in china has changed from subjective weather event forecasts to a combination of both subjective and objective quantitative precipitation forecasts,and is now advancing toward probabilistic quantitative precipitation forecasts with the provision of forecast uncertainty information.

关键词： heavy rainfall reform and opening-up of new china physical mechanisms forecasting techniques

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Consistency of global warming trends strengthened since 1880s

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Science Bulletin 2020年第20期65卷 1709-1712,M0003页

作者： Qjngxiang Li Wenbin sun Boyin Huang Wenjie Dong Xiaolan Wang Panmao Zhai Phil Jones School of Atmospheric Sciences and guangdong Province Key Laboratory for Climate Change and Natural Disasters Sun Yat-sen UniversityGuangzhou 510275China Key Laboratory of Tropical Atmosphere-Ocean System(sun yat-sen university) Ministry of Educationand Southern Laboratory of Ocean Science and Engineering(Zhuhai)Zhuhai 519082China National centers for environmental Information National Oceanic and Atmospheric AdministrationAsheville NC 28801USA Climate Research Division.environment and Climate Change Canada Toronto M3H5T4Canada Chinese Academy of Meteorological Sciences China Meteorological AdministrationBeijing 100081China Climatic Research Unit School of Environmental SciencesUniversity of East AngliaNorwich NR47TJUK

Global mean surface temperature(GMST)is one of the most important large-scale indicators for characterizing climate change on earth,and Surface Temperature(ST)is also the most accurate key climate element currently understood by scientists and the *** so,there have been extensive discussions about the accuracy of global(regional)surface temperature(air temperature)changes[*** the perspective of climatic data acquisition and data reliability,the current GMST series and the evaluation of global warming rates are all based on several observation-based datasets produced by combining anomalies of Land Surface Air Temperatures(LSAT)and Sea Surface Temperatures(SST).

关键词：全球变暖趋势全球温度年均温度观测序列基准数据 IPO EOF分析特征向量

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大理洱海旅游环境治理事件中的主体博弈与权益协商

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地理科学 2020年第9期40卷 1468-1475页

作者：孙九霞陈景黄秀波中山大学旅游学院/中山大学旅游休闲与社会发展研究中心广东广州510275 广东财经大学地理与旅游学院广东广州510320

以大理洱海环境治理事件为典型案例,基于半结构式访谈、观察法等质性方法,从环境正义视角切入分析洱海环境治理中的主体博弈与权益协商议题。研究发现,洱海的环境治理是一种以环境名义开展地社会治理与目的地管理过程,逐渐从治理水环境... 详细信息

以大理洱海环境治理事件为典型案例,基于半结构式访谈、观察法等质性方法,从环境正义视角切入分析洱海环境治理中的主体博弈与权益协商议题。研究发现,洱海的环境治理是一种以环境名义开展地社会治理与目的地管理过程,逐渐从治理水环境延伸至环境、民生、产业升级等多方面的综合治理;洱海治理隐含权力张力与利益冲突,本地村民、旅游经营者及地方基层政府三方基于合情、合理与合法性原则,围绕"情、理、法"逻辑进行利益博弈与协商;乡村旅游中的环境治理应置于乡村与社会治理框架体系下进行,关照民生与发展。环境正义理论可解释乡村旅游中的环境治理与冲突现象,为揭示环境治理背后的利益冲突与社会问题提供独特视角。

关键词：环境治理环境正义乡村旅游权力博弈大理洱海

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The hidden risk of microplastic-associated pathogens in aquatic environments

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Eco-environment & Health 2023年第3期2卷 142-151页

作者： Huan Zhong Mengjie Wu Christian Sonne Su Shiung Lam Raymond W.M.Kwong Yuelu Jiang Xiaoli Zhao Xuemei sun Xuxiang Zhang Chengjun Li Yuanyuan Li Guangbo Qu Feng Jiang Huahong Shi Rong Ji Hongqiang Ren School of environment Nanjing UniversityState Key Laboratory of Pollution Control and Resource ReuseNanjing 210023China Department of Bioscience Arctic Research CentreAarhus UniversityRoskildeDenmark Higher Institution Centre of Excellence(HICoE) Institute of Tropical Aquaculture and Fisheries(AKUATROP)Universiti Malaysia Terengganu21030 Kuala NerusTerengganuMalaysia Department of Biology York University4700 Keele StreetTorontoON M3J 1P3Canada Tsinghua Shenzhen International Graduate School Shenzhen 518055China State Key Laboratory of environmental Criteria and Risk Assessment Chinese Research Academy of Environmental SciencesBeijing 100012China Yellow Sea Fisheries Research Institute Chinese Academy of Fishery SciencesQingdao 266071China Institute of environmental Research at Greater Bay Area Key Laboratory for Water Quality and Conservation of the Pearl River DeltaMinistry of EducationGuangzhou UniversityGuangzhou 510006China Key Laboratory of environment and Health(HUST) Ministry of Education&Ministry of Environmental Protectionand State Key Laboratory of Environmental Health(Incubation)School of Public HealthTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China State Key Laboratory of environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China School of environmental Science and Engineering Guangdong Provincial Key Lab of Environmental Pollution Control and Remediation TechnologySun Yat-sen UniversityGuangzhou 510275China State Key Laboratory of Estuarine and Coastal Research East China Normal UniversityShanghai 200241China university Centre for Research and Development Department of ChemistryChandigarh UniversityGharuanMohaliPunjabIndia

Increasing studies of plastisphere have raised public concern about microplastics(MPs)as vectors for pathogens,especially in aquatic ***,the extent to which pathogens affect human health through MPs remains unclear,as controversies persist regarding the distinct pathogen colonization on MPs as well as the transmission routes and infection probability of MP-associated pathogens from water to *** this review,we critically discuss whether and how pathogens approach humans via MPs,shedding light on the potential health risks *** on cutting-edge multidisciplinary research,we show that some MPs may facilitate the growth and long-range transmission of specific pathogens in aquatic environments,ultimately increasing the risk of infection in *** identify MP-and pathogen-rich settings,such as wastewater treatment plants,aquaculture farms,and swimming pools,as possible sites for human exposure to MP-associated *** review emphasizes the need for further research and targeted interventions to better understand and mitigate the potential health risks associated with MP-mediated pathogen transmission.

关键词： Microplastics Pathogens Colonization Transmission Public health

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Cancer metabolism and tumor microenvironment:fostering each other?

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Science china(Life Sciences) 2022年第2期65卷 236-279页

作者： Yiyuan Yuan Huimin Li Wang Pu Leilei Chen Dong Guo Hongfei Jiang Bo He Siyuan Qin Kui Wang Na Li Jingwei Feng Jing Wen Shipeng Cheng Yaguang Zhang Weiwei Yang Dan Ye Zhimin Lu Canhua Huang Jun Mei Hua-Feng Zhang Ping Gao Peng Jiang Shicheng Su Bing sun Shi-Min Zhao Obstetrics&Gynecology Hospital of Fudan university State Key Laboratory of Genetic EngineeringFudan UniversityShanghai 200438China State Key Laboratory of Cell Biology Center for Excellence in Molecular Cell ScienceChinese Academy of SciencesShanghai 200031China Molecular and Cell Biology Lab Institutes of Biomedical Sciences and School of Life SciencesFudan UniversityShanghai 200032China Zhejiang Provincial Key Laboratory of Pancreatic Disease The First Affiliated Hospitaland Institute of Translational MedicineZhejiang University School of MedicineHangzhou 310029China West china School of Basic Medical Sciences&Forensic Medicine Sichuan Universityand State Key Laboratory of Biotherapy and Cancer CenterWest China Hospitaland Collaborative Innovation Center for BiotherapyChengdu 610041China Department of Biochemistry and Molecular Cell Biology Shanghai Key Laboratory of Tumor Microenvironment and InflammationShanghai Jiao Tong University School of MedicineShanghai 200025China guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation Medical Research CenterSun Yat-Sen Memorial HospitalSun Yat-Sen UniversityGuangzhou 510120China School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China CAS Centre for Excellence in Cell and Molecular Biology the CAS Key Laboratory of Innate Immunity and Chronic DiseaseSchool of Basic Medical SciencesDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230027China School of Medicine Institutes for Life SciencesSouth China University of TechnologyGuangzhou 510006China Tsinghua university School of Life Sciences and Tsinghua-Peking Center for Life SciencesBeijing 100084China

The changes associated with malignancy are not only in cancer cells but also in environment in which cancer cells *** reprogramming supports tumor cells’high demand of biogenesis for their rapid proliferation,and helps tumor cells to survive under certain genetic or environmental *** evidence suggests that metabolic alteration is ultimately and tightly associated with genetic changes,in particular the dysregulation of key oncogenic and tumor suppressive signaling *** cells activate HIF signaling even in the presence of oxygen and in the absence of growth factor *** cancer metabolic phenotype,described firstly by German physiologist Otto Warburg,ensures enhanced glycolytic metabolism for the biosynthesis of *** conception of metabolite signaling,i.e.,metabolites are regulators of cell signaling,provides novel insights into how reactive oxygen species(ROS)and other metabolites deregulation may regulate redox homeostasis,epigenetics,and proliferation of cancer ***,the unveiling of noncanonical functions of metabolic enzymes,such as the moonlighting functions of phosphoglycerate kinase 1(PGK1),reassures the importance of metabolism in cancer *** metabolic,microRNAs,and ncRNAs alterations in cancer cells can be sorted and delivered either to intercellular matrix or to cancer adjacent cells to shape cancer microenvironment via media such as *** them,cancer microenvironmental cells are immune cells which exert profound effects on cancer *** of all these processes is a prerequisite for the development of a more effective strategy to contain cancers.

关键词： cancer metabolism cancer microenvironment epigenetics cancer immunology

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中介、身体与情感：川藏公路旅行中的流动性体验

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地理科学 2019年第11期39卷 1780-1786页

作者：王学基孙九霞黄秀波中山大学旅游学院/中山大学旅游休闲与社会发展研究中心广东广州510275 广东财经大学地理与旅游学院广东广州510320

新流动性范式下,与交通行为相伴的流动不再只是旅游者到达目的地的方式,转而成为深刻嵌入于旅游通道空间中的独特体验。通过在与研究对象一起流动的过程中运用访谈法和观察法收集资料,基于流动性视角探讨川藏公路旅行者在道路空间流动... 详细信息

新流动性范式下,与交通行为相伴的流动不再只是旅游者到达目的地的方式,转而成为深刻嵌入于旅游通道空间中的独特体验。通过在与研究对象一起流动的过程中运用访谈法和观察法收集资料,基于流动性视角探讨川藏公路旅行者在道路空间流动过程中的多元体验及体验间的相互影响关系。研究发现:流动性体验构成了道路旅行者旅游体验的核心,并涉及中介、身体与情感的融合与互动。物质性中介塑造了道路旅行者的中介体验,在构建"连结"和制造"分离"中影响旅行者在人地互动中的身体体验。同时,流动过程塑造了旅行者从视觉到多感官的具身性体验,包含正面与负面体验的交替与综合,且时常涉及危险与疼痛的极致体验。最后,中介与身体体验形成向情感的投射,道路空间环境与流动状态的转换塑造情感体验的多变性。

关键词：流动性旅游体验道路旅行川藏公路

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