检索结果-南通市图书馆

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Xi CHEN,Rui-Ping PANG,Kai-Feng SHEN,Yong-Yong LI,Xian-Guo LIU~* Pain research center and Department of Physiology,Zhongshan School of Medicine of Sun Yat-Sen University,Guangzhou 510080,China

＜正＞A large body of evidence has demonstrated that the ectopic discharges of action potentials in uninjured DRG neurons produced by peripheral nerve injury are important for the development of neuropathic pain and that the abnormal expression of voltage gated sodium channels in DRG neurons is responsible for the ectopic ***,how nerve injury affects the expression of sodium channels is largely *** the present work,we tested if selective damage of motor neurons,leaving sensory neuron intact,by L5 ventral root transection（L5 VRT） increases the function of TTX-sensitive（TTX-S） and TTX-resistant（TTX-R） sodium channels in L4 and L5 DRG *** found that the current density（pA/pF） of the TTX-S in control group was 173.5±19.1（n=8） and was increased significantly at 7 d（2.3.2.14.3,n=7）,14 d（2.0.5±2..3,n=5） after L5 *** current density of the Nav1.8 at 7 d after L5 VRT was 102.3±19.7（n=7）, at 14 d was 111.4±6.6（n=6） respectively,which was significantly higher than 59.9±5.2.n=10） recorded in control group. The current density of the Navl.9 at 14 d（101.3±2..7,n=5） but not at 7 d（63.3±9.9,n=8） after L5VRT was significantly higher than 62.8±5.5（n=12. in control *** our previous work showed that TNF-αis up-regulated in DRGs following L5 VRT,we next tested if the increased TNF-αis responsible for the change in the current densities of sodium channels by pre-treatment of DRG neurons with rrTNF（1ng/ml for 4-12.h）.The results showed that the current density of TTX-S,Navl.8 and Navl.9 was 2.7.5±15.7（n=12.,131.8±8.9（n=14） and 90.7±9.1（n=15） respectively,which was significantly higher than those recorded in control rats mentioned *** data suggest that motor fiber injury is capable of increasing the currents of both TTX-S and TTX-R sodium channels in uninjured sensory neurons and that the increased TNF-αmay be involved in the pathological change.

关键词： neuropathic pain sodium channel dorsal root ganglion tumor necrosis factor-alpha motor

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

植物甾醇对油脂中VA体外模拟控制性释放的影响

引用

中国食品学报 2018年第3期18卷 37-43页

作者：颜亚真傅彦君方超逸傅红张虹郑超福州大学生物科学与工程学院福州350108 福州大学酶工程研究所福州350108 丰益(上海)生物技术研发中心有限公司上海200137

以植物甾醇为有机凝胶剂来制备人造奶油产品,研究体外模拟消化模型中不同质量分数(%)的植物甾醇(0%~7%)对VA释放速率的影响,并通过测定扩散系数及侵蚀常数来解释其动力学机制。试验结果显示,VA释放速率随植物甾醇质量百分数升高而降低,... 详细信息

以植物甾醇为有机凝胶剂来制备人造奶油产品,研究体外模拟消化模型中不同质量分数(%)的植物甾醇(0%~7%)对VA释放速率的影响,并通过测定扩散系数及侵蚀常数来解释其动力学机制。试验结果显示,VA释放速率随植物甾醇质量百分数升高而降低,体外模拟消化2.h时甾醇质量分数7%的奶油中VA的释放率比空白组降低2.%;Rritger-Peppas方程拟合结果表明当甾醇质量百分数为0%~5%时,VA扩散为非Fick扩散;VA浓度和甾醇质量百分数是扩散的主要驱动力,扩散系数随甾醇质量百分数的升高而减小;另外,侵蚀速率随甾醇质量百分数的升高而降低,当甾醇质量百分数为7%时,奶油侵蚀常数比空白组小3倍;随着储存时间延长,储存温度降低,侵蚀常数逐渐减小;当人造奶油中添加单双硬脂酸甘油酯时侵蚀常数约为分子蒸馏单甘脂的2.。

关键词：植物甾醇模拟胃肠道消化释放速率扩散系数侵蚀常数

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of vagus nerve stimulation on heroin seeking in a model of relapse in rats and the underlying neurobiological mechanisms

Effects of vagus nerve stimulation on heroin seeking in a mo...

引用

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Miao-Jun LAI,An-Na SUN,Hua-Qiang ZHU,Hui-Feng LIU,Wen-Hua ZHOU~* Laboratory of Behavioral Neuroscience,Ningbo Addiction research and Treatment center,School of Medicine,Ningbo University,Ningbo 315010,China

＜正＞Objective The present study was performed to investigate the effects of vagus nerver stimulation（VNS） on drug-seeking elicited by cues or heroin,and observed the expression of transcriptional factors in NAc after the VNS *** The rats were trained to acquire heroin self-administration under fixed ratio reinforcement schedule （FR1）.After daily 4h self-administration training session for 10 consecutive days,rats that acquired heroin self-administration were divided into 3 groups:operation group,low intensity vagus nerver stimulation group and high intensity vagus nerver stimulation *** group was treated correspondingly once a day for 10 days,following treatment,rats were put into the previously training cages with environmental *** the last treatment and extinct training,the rats were tested for reinstatment of the drug seeking induced by conditional cues or heroin in the trainning cages for *** the trial,Western blotting was applied to detect the expression of p-CREB protein in related brain *** Active pokes induced by the conditioned cues in low intensity VNS group（56.9±10.9） and high intensivity VNS group（46.7±7.1） were decreased markedly than the operation group（166.2.2..9）（P＜0.01） in the two hours of test *** induced by heroin priming,active pokes of L-VNS group（13.0±4.8） and H-VNS group（15.2.5.2. were decresed clearly than the operation group（32.5±4.7）（P＜0.01）.The inactive pokes between three groups are insignificantly different either in conditional cues or heroin induced test session（P＞0.05）.In the Western blotting experiment,p-CREB was incresed dramatically in NAc of the low or high intensity VNS group compared with the operation group either in conditional cues or heroin induced test *** The present data demonstrated that vagus nerver stimulation could inhibit the relapse of the drug seeking behavior induced by conditioned cues and heroin *** mechanism may be involved in the

关键词： vagus nerver stimulation p-CREB relapse NAc

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

组织光声弹性成像

引用

中国激光 2018年第3期45卷 100-109页

作者：王成董肖娜蔡干项华中郑刚张大伟上海理工大学医疗器械与食品学院生物医学光学与视光学研究所上海200093 上海理工大学教育部光学仪器与系统工程研究中心上海市现代光学系统重点实验室上海200093

触诊和弹性成像是测量组织硬度、反映疾病状态的常见医学诊断方法,在现有的组织弹性成像技术中超声弹性成像已经相当成熟。光声成像利用组织吸收脉冲光产生的光声信号进行成像,兼具光学成像高选择性和纯超声成像高成像深度的优点;弹性... 详细信息

触诊和弹性成像是测量组织硬度、反映疾病状态的常见医学诊断方法,在现有的组织弹性成像技术中超声弹性成像已经相当成熟。光声成像利用组织吸收脉冲光产生的光声信号进行成像,兼具光学成像高选择性和纯超声成像高成像深度的优点;弹性成像是将材料的弹性信息转换成图像,一般分为诱导形变、测量形变和形成图像这几个步骤。基于光声技术的弹性探测及成像的机理和技术已有报道,回顾了当前光声弹性探测的研究成果。在此基础上,基于光声信号本身即为组织热弹性形变产生的超声波,即超声波的传输与组织的弹性特性相关,提出基于光声信号的组织弹性分析,结合光声的功能成像实现功能和弹性的多功能成像,为疾病,特别是肿瘤的诊断与治疗提供更精确的信息。

关键词：生物光学光声成像弹性生物组织多功能

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Changes of glutamatergic receptors in nucleus accumbens induced by conditioned cues during the early or late withdrawal in a heroin relapse model

Changes of glutamatergic receptors in nucleus accumbens indu...

引用

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Hui-Fen LIU,Anna Sun,Miao-Jun LAI,Hua-Qiang ZHU,Ding-Ding ZHUANG,Wen-Hua ZHOU~* Laboratory of Behavioral Neuroscience,Ningbo Addiction research and Treatment center,School of Medicine,Ningbo University,Ningbo 315010,China

＜正＞Objective The glutamatergic projection from the prefrontal cortex to nucleus accumbens（NAc） is crucial to the reinstatement of cocaine or heroin seeking,and the expression of glutamate receptors in NAc is involved in the process of cocaine or heroin *** has been suggested that cue-induced heroin seeking increased following early or late withdrawal from heroin *** we aim to explore the express of glutamate receptors,postsynaptic density protein of PSD95 and the downstream of extracellular signal-regulated kinase（ERK） activity in the reinstatement of heroin induced by *** Rats were trained to self-administer heroin for 10 days,and then assessed nose pokes induced by context cues or by stimulus cues after 1 or 14 withdrawal *** blotting was applied to detect the expression of proteins in related brain *** We found that the active responding induced by cues was higher after 14 withdrawal days than that of 1 day withdrawal,and the active responding induced by cues was no different between 14 withdrawal days and 14 extinction ***,the expressions of glutamate receptors GluR1,NMDAR2. PSD95 and the phospho-ERK,but not GluR2.or NMDAR1 increased following the late *** The present results suggest that the enhancement of GluR1,NMDAR2.expression may be involved in the incubation of heroin seeking induced by cues over the extend withdrawal periods,and postsynaptic density protein of PSD95 may assist these two receptors’ aggregations.

关键词： glutamate heroin drug-seeking extracellular signal-regulated kin

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Proteomic difference between spinal cord stem cells and motor neurons

Proteomic difference between spinal cord stem cells and moto...

引用

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Peng-Fei LI,Chun-Fang WANG~*,Fei WANG,Hong-En WEI Department of biotechnology,Shanxi Medical University,Taiyuan 030001,China

＜正＞Objective Motor neuron damage always occurs in amyotrophic lateral sclerosis and spinal cord *** research offers hope that neural stem cells（NSCs） may be donors to replace lost motor *** vitro,we proved that NSCs can differentiate into cholinergic neurons（motor neuron is a type of cholinergic neuron） in a special condition. Others have shown that some factors could induce mouse embryonic stem（ES） cells to differentiate into motor neurons. Since proteomic approaches are most appropriate to determine protein content,stability and subcellular localization,many people used the 2.DE comparative proteome analysis to study the differentiation process of *** some learned details of the differentiation process,questions that whether these differentiated cells are really the ones which we want remain ***,before inducing NSCs into motor neurons,we should find the difference between *** this paper,we used the proteomic analysis to gain more insight into molecular mechanisms of the difference between NSCs and motor *** cord stem cells（SCSCs） are a subclass of NSCs and as such are restricted to generating neural tissues,which is a notable advantage over embryonic stem cells,and have the further advantage of being regionally specified to produce spinal cord *** SCSCs and motor neurons obtained from the same 16-day-old rat fetus spinal cord,this can make our result have more obviously biological statistical ***,we employed the method of difference gel electrophoresis（DIGE）,which allows protein differences between two samples to be analyzed on the same *** the transiently expressed p75-NGFR was chosen for motor neuron isolation from embryonic day 15 to postnatal day 15 rat spinal cords,we used CELLection Pan Mouse IgG Kit to separate motor neurons,and we used the rest spinal cord after motor neurons separation to SCSCs *** cell lysate preparation,we did 2. gel analysis according

关键词： neural stem cells motor neurons proteome

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

The direction of synaptic plasticity mediated by C-fibers in spinal dorsal horn is decided by Src-family kinases in microglia

The direction of synaptic plasticity mediated by C-fibers in...

引用

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Yi ZHONG,Li-Jun ZHOU,Wen-Jie REN,Wen-Jun XIN,Yong-Yong LI,Tong ZHANG,Xian-Guo LIU~* Pain research center and Department of Physiology,Zhongshan School of Medicine,Sun Yat-sen University,Guangzhou 510080,China

＜正＞Previous studies have shown that Src-family kinases（SFKs） are selectively activated in spinal microglia following peripheral nerve injury,which play a key role in the development of neuropathic *** investigate the underlying mechanism,in the present study the role of SFKs in synaptic plasticity mediated by afferent C-fibers in spinal dorsal horn was evaluated in adult *** data revealed that pretreatment with either microglia inhibitor（minocycline, 2.0μM） or SFKs inhibitors（PP2.100μM and SU6656,2.0μM） reversed the effect of high frequency stimulation（HFS）, that is,HFS,which induces long-term potentiation（LTP） normally,induced long-term depression（LTD） after inhibition of either microglia or *** blotting analysis showed that the level of phosphorylated SFKs（p-SFKs） in ipsilateral spinal dorsal horn was transiently increased after LTP induced by HFS,starting at 15 min and returning to control level at 60 min after *** immunofluorescence staining demonstrated that p-SFKs were highly restricted to microglia. Furthermore,we found that the inhibitory effects of minocycline or SU6656 on spinal LTP were reversed by spinal application of rat recombinant TNF-α（500 pg/mL） and that HFS failed to induce LTP of C-fiber evoked field potentials in TNFRl’" *** results suggest that activated microglia may control the direction of plastic changes at C-fiber synapses in spinal dorsal horn via release of TNF-α.

关键词： synaptic plasticity long-term potentiation long-term depression microglia

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Electroacupuncture treatment reverses morphine-induced physiological changes in dopaminergic neurons within the ventral tegmental area

Electroacupuncture treatment reverses morphine-induced physi...

引用

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Ling HU,Ning-Ning CHU,Lin-Lin SUN,Rong ZHANG,Ji-Sheng HAN,Cai-Lian CUI~* Neuroscience research Institute and Department of Neurobiology,Peking University Health Science center,Key Laboratory of Neuroscience,the Ministry of Education and Ministry of Public Health,Beijing 100191,China

Chronic morphine administration decreases the size of dopamine(DA) neurons in the ventral tegmental area (VTA).These transient morphological changes are accompanied by a reduced sensitivity of morphine-induced conditioned place preference(CPP) after chronic exposure to the *** this study we examined alterations in the firing rate of DAergic neurons by means of extracellular recording following chronic morphine exposure and applied 100 Hz electroacupuncture(EA) treatment to reverse the reduced firing rate of these *** the first set of experiments we show that in rats,which received chronic morphine treatment for 14 days,a small dose of morphine was not able to induce a CPP response ***,the sensitivity to morphine was reinstated by consecutive EA treatment for 10 days. The electrophysiological response of VTA DA neurons to morphine was markedly reduced in chronic morphine-treated rats compared to saline-treated controls.A substantial recovery of the reactivity of VTA DA neurons to morphine was observed in rats that received 100 Hz EA for 10 *** findings suggest that 100 Hz EA is a potential therapy for the treatment of opiate addiction by normalizing the activity of VTA DA neurons.

关键词： conditioned place preference(CPP) dopamine neurons electroacupuncture(EA) morphine ventral tegmental area

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

A validation of a potential biomarker of PD focus on phosphorylatedα-synuclein at 12. serine

A validation of a potential biomarker of PD focus on phospho...

引用

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Nian-Qiang WANG,Ji-Sheng LI,Hui YANG~* Beijing Institute for Neuroscience,Capital Medical University,Beijing center for Neural Regeneration & Repair,Key Laboratory for Neurodegenerative Disease of the Ministry of Education,Beijing 100069,China

＜正＞Objective Synuclein is a protein associated with familial and sporadic PD.α-Synuclein is hyperphosphorylated at 12. serine in PD patients and animal models,approximately 90%in Lewy bodies,while only 4%normally;and phosphorylated a-synuclein is the main form of *** the brain,α-synuclein is widely expressed,but it is especially neurotoxic to dopaminergic neurons in the substantia nigra pars compacta（SNpc）.This may be due to the fact that nigral dopaminergic neurons are particularly exposed to oxidative stress due to DA *** present there are a few commercial antibodies against phosphorylatedα-synuclein and are less efficient for PD studies,furthermore no laboratory test that correlates with PD risk is *** tests for PD are critically needed for developing treatment designed to slow or prevent progression of the *** biomarkers of PD are also attracted more and more attention, we prepare monoclonal antibodies against phosphorylatedα-synuclein at Serl2.,and establish a new in vitro diagnosis of PD Detection Kit for study on the pathogenesis of PD in future as well as on validation of phosphorylatedα-synuclein as a biomarker for early diagnosis of *** To obtain the antiserum against phosphorylatedα-synuclein at Serl2., two Blb/c mice were immunized with 14 amino acids（EMPpSEEMPpSEE-GYQ）as hapten which coupled with *** carbodiimide（EDC） coupling chemical technique was used to form a coupling agent（peptide-mcKLH） by GL Biochem （shanghai）.The B cells taken from spleens of the mice were fused with myeloma cell（SP2.Cell）.Results After sufficient screening and testing work by ELISA,western-blot and immunoblot,we obtained the ideal monoclonal antibody of phosphorylatedα-synuclein at *** Subsequently,our next work will focus on the establishment of a new in vitro diagnosis of PD Detection Kit,to make any contribution to PD research.

关键词： PD monoclonal antibodies phosphorylatedα-synuclein dopaminergic Lewy bodies biomarker

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：