Effects of vagus nerve stimulation on heroin seeking in a model of relapse in rats and the underlying neurobiological mechanisms
会议名称：《中国神经科学学会第四次会员代表大会暨第八届全国学术会议》
会议日期：2009年
学科分类：1002[医学-临床医学] 100205[医学-精神病与精神卫生学] 10[医学]
基 金：supported by the National Natural Science Foundation of China(No.2009CB522008) Natural Science Foundation of China(No.30670675), Zhejiang Province(No.D208015)
关 键 词：vagus nerver stimulation p-CREB relapse NAc
摘 要：正Objective The present study was performed to investigate the effects of vagus nerver stimulation（VNS） on drug-seeking elicited by cues or heroin,and observed the expression of transcriptional factors in NAc after the VNS *** The rats were trained to acquire heroin self-administration under fixed ratio reinforcement schedule （FR1）.After daily 4h self-administration training session for 10 consecutive days,rats that acquired heroin self-administration were divided into 3 groups:operation group,low intensity vagus nerver stimulation group and high intensity vagus nerver stimulation *** group was treated correspondingly once a day for 10 days,following treatment,rats were put into the previously training cages with environmental *** the last treatment and extinct training,the rats were tested for reinstatment of the drug seeking induced by conditional cues or heroin in the trainning cages for *** the trial,Western blotting was applied to detect the expression of p-CREB protein in related brain *** Active pokes induced by the conditioned cues in low intensity VNS group（56.9±10.9） and high intensivity VNS group（46.7±7.1） were decreased markedly than the operation group（166.2±20.9）（P0.01） in the two hours of test *** induced by heroin priming,active pokes of L-VNS group（13.0±4.8） and H-VNS group（15.2±5.2） were decresed clearly than the operation group（32.5±4.7）（P0.01）.The inactive pokes between three groups are insignificantly different either in conditional cues or heroin induced test session（P0.05）.In the Western blotting experiment,p-CREB was incresed dramatically in NAc of the low or high intensity VNS group compared with the operation group either in conditional cues or heroin induced test *** The present data demonstrated that vagus nerver stimulation could inhibit the relapse of the drug seeking behavior induced by conditioned cues and heroin *** mechanism may be involved in the