The direction of synaptic plasticity mediated by C-fibers in spinal dorsal horn is decided by Src-family kinases in microglia
会议名称：《中国神经科学学会第四次会员代表大会暨第八届全国学术会议》
会议日期：2009年
学科分类：0710[理学-生物学] 07[理学] 071006[理学-神经生物学]
关 键 词：synaptic plasticity long-term potentiation long-term depression microglia
摘 要：正Previous studies have shown that Src-family kinases（SFKs） are selectively activated in spinal microglia following peripheral nerve injury,which play a key role in the development of neuropathic *** investigate the underlying mechanism,in the present study the role of SFKs in synaptic plasticity mediated by afferent C-fibers in spinal dorsal horn was evaluated in adult *** data revealed that pretreatment with either microglia inhibitor（minocycline, 200μM） or SFKs inhibitors（PP2,100μM and SU6656,200μM） reversed the effect of high frequency stimulation（HFS）, that is,HFS,which induces long-term potentiation（LTP） normally,induced long-term depression（LTD） after inhibition of either microglia or *** blotting analysis showed that the level of phosphorylated SFKs（p-SFKs） in ipsilateral spinal dorsal horn was transiently increased after LTP induced by HFS,starting at 15 min and returning to control level at 60 min after *** immunofluorescence staining demonstrated that p-SFKs were highly restricted to microglia. Furthermore,we found that the inhibitory effects of minocycline or SU6656 on spinal LTP were reversed by spinal application of rat recombinant TNF-α（500 pg/mL） and that HFS failed to induce LTP of C-fiber evoked field potentials in TNFRl’ *** results suggest that activated microglia may control the direction of plastic changes at C-fiber synapses in spinal dorsal horn via release of TNF-α.