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Identification of a unique 312.nucleotides variant of Hop stunt viroid（HSVd） with a tandem 15 nucleotides repeat from plum

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Identification of a unique 312 nucleotides variant of Hop st...

中国植物保护学会2.07年学术年会

作者： Yuanai Yang~(1,2. Hongqing Wang~2.Zujian WU~3 Zhuomin Cheng~1 Shifang Li~(1*) (1 State key laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural {2.s,Yuanmingyuan West No.2. beijing 100094,People''s Republic of china 2.department of Fruit {2., College of Agronomy and biotechnology,china Agricultural university,beijing, People''s Republic of china.3 Institute of Plant Virology,Fujian Agriculture and Forestry university,Fuzhou 350002.china)

＜正＞Sixty-eight plum samples were collected from 12.different provinces of *** molecular weight RNAs were extracted and used for dot-blot,reverse transcription-polymerase chain reaction （RT-PCR）,return-polyacrylamide gel electrophoresis（R-PAGE）,and biological indexing using cucumber .Results showed that 15 of the 68 plum samples were positive for Hop stunt viroid（HSVd）.Four positive samples were selected for cloning and sequence *** indicated that most HSVd sequences from the plum in china had 1～3 nucleotides changes from the closest HSVd in *** addition,the sample PB2.（cv."Friar"） collected from Hebei province had one sequence with a 15 nt duplication （named HSVd D-15） at the 2.4/2.5 position in the lower central *** biological indexing,cucumber seedlings,an indicator plant for HSVd,were inoculated with RNAs directly extracted from the original plum source（PB 2.,cv."Friar"）.Nucleotide sequencing analysis of the progeny showed that HSVd but not HSVd-D15,was recovered from inoculated *** very unlikely,the possibility that this extra sequence was the result of a PCR artifact cannot be completely ruled out.

关键词： Hop stunt viroid plum recombination sequence repeat

A comparison of optimization theories for energy conservation in heat exchanger groups

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science Bulletin 2011年第Z1期 449-454页

作者： CHEN Qun1,2.WU Jing3,WANG MoRan4,PAN Ning2.& GUO ZengYuan1 1 key laboratory for Thermal {2. and Power Engineering of Ministry of Education,department of Engineering Mechanics,tsinghua university,beijing 100084,china 2.department of biological and Agricultural Engineering,university of California,Davis CA 95616,USA 3 College of Energy and Power Engineering,Nanjing university of Aeronautics and Astronautics,Nanjing 2.0016,china 4 Earth and Environmental {2.s Division,Los Alamos National laboratory,Los Alamos NM 87545,USA

In general,thermal processes can be classified into two categories: heat-work conversion processes and heat transfer processes. Correspondingly,the optimization of thermal processes has to have two different criteria:the well known entropy generation minimization method and the recently proposed entransy dissipation maximization method. This study analyzes the thermal issues in a heat exchanger group,and optimizes the unit arrangements under different constraints based on a suitable optimization crite-rion. The result indicates that the principle of minimum entropy generation rate is valid for optimizing heat exchangers in a ther-modynamic cycle with given boundary temperatures. In contrast,the entransy dissipation maximization is more suitable in heat exchanger optimizations involving only heat transfer processes. Furthermore,the entropy generation rate induced by dumping used streams into ambient surroundings has to be taken into account,except for that originating from the hot and cold-ends of heat exchangers,when using the entropy generation minimization to optimize heat exchangers undergoing a thermodynamic cycle.

关键词： heat exchangers optimization criterion entransy dissipation maximization entropy generation minimization

TFAR19促进小鼠肝线粒体膜通透性转运孔的开放

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生物化学与生物物理学报 2002年第3期34卷 279-284页

作者：田辉凯夏天蒋春笋张红梅王坤李学军北京大学医学部基础医学院药理学系北京大学生命科学学院生理生物物理系生物膜国家重点实验室天津体育学院运动医学研究所北京大学医学部基础医学院药理学系北京100083

TFAR19基因 (TF 1cellapoptosisrelatedgene 19)是北京大学人类疾病基因中心从人白血病细胞株TF 1细胞中克隆到的凋亡相关新基因之一 (GenBank登记号AF0 1495 5 )。初步研究发现 ,该基因在细胞凋亡时高表达 ,并且表达产物具有抑制肿瘤... 详细信息

TFAR19基因 (TF 1cellapoptosisrelatedgene 19)是北京大学人类疾病基因中心从人白血病细胞株TF 1细胞中克隆到的凋亡相关新基因之一 (GenBank登记号AF0 1495 5 )。初步研究发现 ,该基因在细胞凋亡时高表达 ,并且表达产物具有抑制肿瘤细胞生长和促进凋亡作用。但是其确切的作用机制不明。线粒体膜完整性破坏所导致促凋亡因子 (如细胞色素c等因子 )的释放是细胞凋亡关键性的控制因素。线粒体膜通透性转运孔 (PTP) ,对线粒体膜完整性具有重要的调控作用。研究了重组人TFAR19蛋白在体外条件下 ,对线粒体PTP、跨膜电位 ,以及细胞色素c释放的影响。结果表明 ,TFAR19蛋白使分离的小鼠肝线粒体PTP开放、线粒体跨膜电位下降 ,以及细胞色素c释放。TFAR19对线粒体的上述作用是通过促进PTP开放起作用的。实验结果提示 ,TFAR19对线粒体凋亡信号有正反馈放大作用 ,并进一步揭示了TFAR19促进细胞凋亡的机制

关键词：凋亡 TFAR19 线粒体跨膜电位膜通透性转运孔(PTP)

Intimal hyperplasia thickness and blood flow in an axis-deviated arterial bypass

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Intimal hyperplasia thickness and blood flow in an axis-devi...

中国畜牧兽医学会2.10年学术年会——第二届中国兽医临床大会

作者： Zaipin Xu~1,Wei Zhao~1,Anqiang Sun~2.Xiaoyan Deng~2.Yubo Fan~2.Qingyu Wang~1, Dingkun Wang~1,Junbo Gao~1,Peng Luo~1,Yao Peng~1,Yuhuan Lv~1,Zhe Ma~1,Ke Wang~3 (1.department of Veterinary medicine,College of Animal science,Guizhou {2., Guiyang 55002.,china 2.key laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of biological science and Medical Engineering, Beihang {2.,beijing 100191,china 3.The First Affiliation Hospital,Dalian Medical {2.,Dalian 116011,china)

Extensive work has shown that slow recirculation flow zone with low wall shear stress and high oscillatory shear index occurred in a bypassed artery is an important factor for the development of intimal hyperplasia(IH) in the distal *** substantiate our hypothesis that intentionally inducing swirling flow in a bypassed artery can suppress IH development along the floor of the host artery,we constructed an axis-deviated arterial bypass(ADAB) on canine common carotid artery in which swirling flow was created in the host artery and compared it with a conventional arterial bypass(CAB) in terms of IH development along the host artery *** flows in the two bypass models were characterized *** animal experiment showed that intimal thickness was 110.50±9.62.m in the CAB group and 60.32.3.52.m in the ADAB group, *** blood flow simulation revealed that the flow structure in the host artery of the ADAB model was in the form of swirling flow that was very different from the vortex flow in the conventional model,which characterized with flow stagnation and low wall shear stress along the host artery *** swirling flow created by the axis-deviated bypass eliminated the stagnation flow and enhanced wall shear stress,hence suppressed IH development along the host artery ***,the present study has pointed out a new way to eliminate and/or suppress the slow recirculation flow zones in bypassed arteries.

关键词： Axis-deviated arterial bypass Bypass surgery Intimal hyperplasia Computational fluid dynamics Swirling flow Canine

Microbial carbonates in Holocene beachrocks,Shuiweiling,Luhuitou Peninsula,Hainan Island

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science china Earth sciences 2008年第1期51卷 30-40页

作者： TENG JianBin1,2.& SHEN JianWei1 1 department of Marine Geology, South china Sea Institute of Oceanology CAS {2. laboratory of Marginal Sea Geology, South china Sea Institute of Oceanology, Chinese Academy of sciences, Guangzhou 510301, china 2.Graduate university of the Chinese Academy of sciences, beijing 100049, china 1. department of Marine Geology South China Sea Institute of Oceanology Chinese Academy of Sciences Guangzhou 510301 China 2. Graduate university of the Chinese Academy of sciences Beijing 100049 China 3. CAS key laboratory of Marginal Sea Geology South China Sea Institute of Oceanology Chinese Academy of Sciences Guangzhou 510301 China

Two research methods, geomicrobiology and carbonate sedimentology, were adopted to study the microbial carbonates in the beachrocks, Shuiweiling, Luhuitou, Sanya City, Hainan Island. The results indicate that microbial carbonates occur in Shuiweiling beachrocks and cements related to microbial activitives are common. Microbial carbonates were mainly developed on the surfaces of coral skeletons and within the coral (fragments and shivers) skeletal cavities,and rarely grew in interspaces formed by coral and other biological detritus. The growth morphologies of microbial carbonates are various, including stromatolitic lamination, thin crusts, dome-like, and microbialites. Microbial carbonates commonly trap fine-sized bioclasts, contain tubular filaments, and show laminated growth striation. Microbial carbonates within the coral skeletal cavities are generally stromatolitic cone-shaped, with a random growth direction along the bearing of trend of cavities, showing distinct growth laminae and recognizable growth phases. It is a special growth pattern of crypt microbial carbonates. Microbial cements occur mainly in shape of needle cement, calcified filaments, microborings, and calcified framboidal spheres. The needle cement is the most common type. These microbial cements indicate that microbial activities and microbial carbonates played an important role in beachrock formation in which carbonates were dominant components and the climate of tropical ocean monsoon was influential. Such microbial carbonates and microbial cementation have not been reported here from beachrocks along the northern shorelines of the South china Sea. The results support a clear microbial origin for certain carbonate cements in beachrocks, confirm that microbial carbonates are a significant contributor to carbonate sedimentation, and widen the knowledge of beachrocks from a new field.

关键词： microbial carbonates microbial cements beachrock Holocene Hainan Island

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Inhalative formaldehyde exposure enhances aggressive behavior and disturbes monoamines in frontal cortex synaptosome of male rats

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Inhalative formaldehyde exposure enhances aggressive behavio...

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Yi LIU~1 Zhan-Lei YE~1 Han-Zhi LUO~1 Miao SUN~1 Mi LI~1 Dong-Sheng FAN~2.De-Hua CHUI~(1 2.*) 1 Neuroscience Research Insitute and department of Neurobiology {2. laboratory for Neuroscience Ministry of Education {2. laboratory for Neuroscience Ministry of Public Health Peking university Health science Center beijing 100191 china. 2.department of Neurology Peking university Third Hospital beijing 100191 china

＜正＞Objective Formaldehyde（FA） exposure is known to be toxic to central nervous system of *** this paper,we evaluated the aggressive behavior after repetitive inhalative FA exposure to male SD rats,and explored the potential *** The rats,ranging from 160-180g,were randomly designated into the orchiectomized（ORX） group,the control and the inhalative FA treatment *** rats underwent orchiectomy *** days after the orchiectomy surgery,the inhalative FA（monitored to be 13.5±1.5ppm） treatment *** We found that the male SD rats,those were exposed to FA showed more aggressive behavior compared to the *** the ORX rats exhibited less aggressive behavior than the ***,HPLC assay revealed that the dopamine increased and 5-HT decreased significantly in frontal cortex synaptosome after inhalative FA *** It is the first to evaluate aggressive behavior and identified monoamines disturbances in the frontal cortex synaptosome after the repetitive inhalative FA exposure to rodents.

关键词： dopamine serotonin frontal cortex synaptosome aggression inhalative formaldehyde exposure

Design,Synthesis and biological Activity of novel steroid and Bioconjugates

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Design,Synthesis and biological Activity of novel steroid an...

第五届全国化学生物学学术会议

作者： Yong JU key laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology,Ministry of Education,{2. of Chemistry,tsinghua university,beijing,100084

＜正＞Steroids play very important role in the life of animals and plants and steroid phosphates are also important biological *** to their lipophilic character,lymphocyte membrane affinity,and the ability of binding to low density lipoproteins,design and synthesis of steroidal bioconjugates as bioactive pro-drugs become important,because such drugs should have increased lipophilicity and enhanced permeability through cell

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The effects of 2.z electroacupuncture on regional cerebral biodistribution of 11C-carfentanil in rats and the pilot study of small animal PET using this tracer

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The effects of 2Hz electroacupuncture on regional cerebral b...

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Xiao-Hui XIANG~1 Jin-Ming ZHANG~2.Jia-He TIAN~2.Ji-Sheng HAN~1 Cai-Lian CUI~(1 *) 1 Neuroscience Research Institute and department of Neurobiology Peking {2. Health science Center key laboratory of Neuroscience the Ministry of Education and Ministry of Public Health beijing 100191 china 2.department of Nuclear Medicine the Chinese PLA General Hospital beijing 100853 china

＜正＞Objective A deal of evidence shows that 2.Hz electroacupuncture（EA） produces a significant increase in the content of enkephalin and endorphin of CNS in mediating *** emission tomography（PET） is an only real time method to studyμ-opioid receptors in vivo at *** purpose of this study is to investigate the effects of 2.Hz EA on regional cerebral biodistribution of 11C-carfentanil in rats and to study whether 11C-carfentanil can be used as a tracer in small animal PET *** Biodistribution studies performed after intravenous 11C-carfentanil injection by weighing and radioactivity counting the samples of eight brain regions（cerebellum,hypothalamus,thalamus, hippocampus,striatum,cortex,midbrain,pons） in *** biodistribution results of control groups at the time point of 0 min,10 min,2. min,30 min and 60min and EA group at 30 min were presented as regional cerebral tissue/cerebellum radioactivity *** were scanned with small animal PET in list mode and the images of corresponding time points were reconstructed in 3D OSEM *** PET results were analyzed using region of interesting（ROI）/ cerebellum radioactivity *** Regional cerebral biodistribution in the rats showed a significantly higher uptake of 11C-carfentanil by the thalamus,hypothalamus and striatum than by the cerebellum,with hippocampus,cortex and midbrain showing middle *** regional cerebral tissue/cerebellum radioactivity ratio reached the peak at the time point of 30 *** with naloxone significantly reduced the uptake of 11C-carfentanil in the regions aforementioned.2.Hz EA significantly decreased tissue/cerebellum radioactivity ratio of hypothalamic nucleus（P = 0.031）.The findings from small animal PET were consistent with the *** These results indicate that 2.Hz EA for 30 min can increase the content of enkephalin and endorphin in hypothalamus and suggest that 11C-carfentanil may be used as a tracer in res

关键词： μ-opioid receptor 11C-carfentanil regional cerebral biodistribution small animal PET electroacupuncture

Nitric oxide induced by hydrogen peroxide mediates ethylene-induced stomatal closure of Arabidopsis thaliana

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Nitric oxide induced by hydrogen peroxide mediates ethylene-...

2.09中国植物学会植物细胞生物学学术年会

作者： Guo-hua Liu~1,Li-xia Hou~1,Jing Liu~1,Xue-chen Wang~2.Xin Liu~(1,2.) 1 department of Life science,Qingdao Agriculture {2.,Changcheng Road 700#, Qingdao 2.6109,P.R.china 2.State key laboratory of Plant Physiology and Biochemistry,College of biological sciences,china Agricultural {2.,Yuanming Yuan West Road 2.,beijing 100094, P.R.china

Stomatal movement is regulated by a complex network of signaling events involving numerous intermediates,among them hydrogen peroxide(HO)and nitric oxide(NO). Here,the sources of NO production,the role of NO and HO du... 详细信息

关键词： Arabidopsis thaliana nitric oxide hydrogen peroxide ethylene stomatal closure