The effects of 2Hz electroacupuncture on regional cerebral biodistribution of 11C-carfentanil in rats and the pilot study of small animal PET using this tracer

作     者：Xiao-Hui XIANG~1 Jin-Ming ZHANG~2 Jia-He TIAN~2 Ji-Sheng HAN~1 Cai-Lian CUI~(1 *) 1 Neuroscience Research Institute and Department of Neurobiology Peking University Health Science Center Key Laboratory of Neuroscience the Ministry of Education and Ministry of Public Health Beijing 100191 China 2 Department of Nuclear Medicine the Chinese PLA General Hospital Beijing 100853 China 

会议名称：《中国神经科学学会第四次会员代表大会暨第八届全国学术会议》

会议日期：2009年

学科分类：1005[医学-中医学] 100512[医学-针灸推拿学] 10[医学] 

基　　金：supported by the National Basic Research Program(2007CB512501) the National Natural Science Foundation(30770690) of China 

关 键 词：μ-opioid receptor 11C-carfentanil regional cerebral biodistribution small animal PET electroacupuncture 

摘      要：正Objective A deal of evidence shows that 2 Hz electroacupuncture（EA） produces a significant increase in the content of enkephalin and endorphin of CNS in mediating *** emission tomography（PET） is an only real time method to studyμ-opioid receptors in vivo at *** purpose of this study is to investigate the effects of 2 Hz EA on regional cerebral biodistribution of 11C-carfentanil in rats and to study whether 11C-carfentanil can be used as a tracer in small animal PET *** Biodistribution studies performed after intravenous 11C-carfentanil injection by weighing and radioactivity counting the samples of eight brain regions（cerebellum,hypothalamus,thalamus, hippocampus,striatum,cortex,midbrain,pons） in *** biodistribution results of control groups at the time point of 0 min,10 min,20 min,30 min and 60min and EA group at 30 min were presented as regional cerebral tissue/cerebellum radioactivity *** were scanned with small animal PET in list mode and the images of corresponding time points were reconstructed in 3D OSEM *** PET results were analyzed using region of interesting（ROI）/ cerebellum radioactivity *** Regional cerebral biodistribution in the rats showed a significantly higher uptake of 11C-carfentanil by the thalamus,hypothalamus and striatum than by the cerebellum,with hippocampus,cortex and midbrain showing middle *** regional cerebral tissue/cerebellum radioactivity ratio reached the peak at the time point of 30 *** with naloxone significantly reduced the uptake of 11C-carfentanil in the regions aforementioned.2 Hz EA significantly decreased tissue/cerebellum radioactivity ratio of hypothalamic nucleus（P = 0.031）.The findings from small animal PET were consistent with the *** These results indicate that 2 Hz EA for 30 min can increase the content of enkephalin and endorphin in hypothalamus and suggest that 11C-carfentanil may be used as a tracer in res