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Progress in Natural Science:Materials International 2006年第12期16卷 1288-1294页

作者： XU Rongqi~ 1,3 , LI Xianzhen~3, WEI Hongyu~3, JIANG Bole~2. LI Kai~3, HE Yongqiang~ 2.3 , FENG Jiaxun~ 1,3 and TANG Jiliang~ 1,2.**(1. Guangxi key laboratory of Subtropical Bioresources Conservation and Utilization {2. 3. College of Life Science and Technology, Guangxi University, Nanning 530004, China) Guangxi key laboratory of Subtropical Bioresources Conservation and Utilization Guangxi University Nanning 530004 China College of Life Science and Technology Guangxi University Nanning 530004 China The key laboratory of {2. of education for microbial and plant genetic engineering Guangxi University Nanning 530004 China

Eight putative avirulence genes in Xanthomonas campestris pv. campestris (Xcc) strain 8004 were characterized by Tn5gusA5 mutagenesis and gene expression analysis. The virulence test of mutants on Chinese radish showed that all mutants in individual avr genes except avrBs2.mutant were not significantly different from the wild type in virulence. The avrBs2.mutant showed reduced virulence and bacterial growth in planta. Gene expression analysis using β-glucuronidase as reporter indicated that avrBs1.1,avrBs1,avrXccB,avrXccC,avrXccE1 were regulated by hrpG, whereas avrXccA1, avrXccA2.and avrBs2.were not. RT-PCR analysis showed that all hrpG-regulated genes except avrBs1 were also regulated by hrpX. In addition, it was demonstrated that avrBs1 was responsible for elicitation of a type III dependent hypersensitive reaction (HR) on nonhost plant pepper ECW-10R, and wild type Xcc 8004 was unable to cause HR on pepper ECW-2.R.

关键词： Xanthomonas campestris pv. campestris avirulence gene virulence expression analysis.

Optimization of Agrobacterium tumefaciens-Mediated Immature Embryo Transformation System and Transformation of Glyphosate-Resistant Gene 2.G2.EPSPS in Maize(Zea mays L.)

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Journal of Integrative Agriculture 2013年第12期12卷 2134-2142页

作者： YU Gui-rong LIU Yan DU Wen-ping SONG Jun LIN Min XU Li-yuan XIAO Fang-ming LIU Yong-shengkey laboratory for Bio-Resource and Eco-Environment {2. of education/State key laboratory of Hydraulics and Mountain River engineering College of Life Science Sichuan University key laboratory for Bio-Resource and Eco-Environment Ministry of Education/State Key Laboratory of Hydraulics and Mountain River EngineeringCollege of Life ScienceSichuan University Institute of Biotechnology & Nuclear Techniques Sichuan Academy of Agricultural Sciences Crop Sciences Research Institute China Academy of Agricultural Sciences Biotechnology Research Institute China Academy of Agricultural Sciences Department of plant Soil and Entomological SciencesUniversity of Idaho Moscow

Since maize is one of the most important cereal crops in the world,establishment of an efficient genetic transformation system is critical for its *** the current study,several elite corn lines were tested for suitability of Agrobacterium tumefaciens-mediated transformation by using immature embryos as *** ability and efficiency of transformation of *** *** EHA105 and LBA4404,different heat treatment times of immature embryos before infection,influence of L-cysteine addition in co-cultivation medium after transformation,and how different ways of selection and cultivation influence the efficiency of transformation were ***-resistant gene 2.G2.EPSPS was transformed into several typical maize genotypes including 78599,Zong 31 and BA,under the optimum *** showed that the hypervirulent Agrobacterium tumefaciens *** EHA105 was more infectious than *** of L-cysteine（100 mg L-1） in co-cultivation medium,and heating of the immature embryos for 3 min prior to infection led to a significant increase in the transformation *** in resting medium for 4-10 d and delaying selection was beneficial to the survival of resistant *** induction of germination,adding a high concentration of 6-BA（5 mg L-1） and a low concentration of 2.4-D（0.2.mg L-1） to regeneration medium significantly enhanced germination *** the optimized transformation procedure,more than 800 transgenic plants were obtained from 78599,Zong 31 and *** spraying herbicide glyphosate on leaves of transgenic lines,we identified 66 primary glyphosate-resistant *** transformation efficiency was 8.2..PCR and Southern-blot analyses confirmed the integration of the transgenes in the maize genome.

关键词： maize immature embryo Agrobacterium tumefaciens-mediated transformation transgenic approach glyphosate resistance

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Cytogenetic comparisons between A and G genomes in Oryza using genomic in situ hybridization

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Cell Research 2006年第3期16卷 260-266页

作者： Zhi Yong Xiong~(1,2. Guang Xuan Tan~1 Guang Yuan He~2.Guang Cun He~1 Yun Chun Song~11 key Laboratoty of the ministry of {2. for plant Developmental Biology,College of Life Sciences,Wuhan University,Wuhan 430072.China.2.China-UK HUST-R Res genetic engineering and Genomics Joint laboratory,Huazhong University of Science and Technology,Wuhan 430074,China keylaboratoryofthe{2.ofeducationforplantDevelopmentalBiology CollegeofLifeSciencesWuhanUniversityWuhan430072China China-UKHUST-RResgeneticengineeringandGenomicsJoint{2. HuazhongUniversityofScience

The genomic structures ofOryza sativa （A genome） and *** （G genome） were comparatively studied usingbieolor genomic in situ hybridization （GISH）.GISH was clearly able to discriminate between the chromosomes of *** and *** in the interspecific F1 hybrids without blocking DNA,and co-hybridization was hardly *** average mitotic chromosome length of *** was found to be 1.69 times that of ***.A comparison of4,6-diamidino-2.phenylindole staining showed that the chromosomes of *** were more extensively labelled,suggesting that the G genome is amplified with more repetitive sequences than the A *** interphase nuclei,9-12.hromocenters were normally detected and nearly all the chromocenters constituted the G genome-specific *** larger chromocenters formed by chromatin compaction corresponding to the G genome were detected in the hybridcompared with its *** pachytene of the F1 hybrid,most chromosomes of A and G did not synapse eachother except tot 1-2.chromosomes paired at the end of their *** meiotic metaphase I,three types of chromosomalassociations,i.e.O,sativa-O,sotiva（A-A）,***-O,meyeriana （A-G） and ***-O,meyeriana （G-G）,wereobserved in the F1 *** A-G chromosome pairing configurations included bivalcnts and *** resultsprovided a foundation toward studying genome organization and evolution of ***.

关键词： Oryza sativa Oryza meyeriana 45S rDNA genomic in situ hybridization

Transcriptomics of abiotic factors-stressed maize

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Transcriptomics of abiotic factors-stressed maize

2.11全国植物生物学研讨会

作者： Youzhi Li~*,Xianwei Fan,Haifen Lu,Dongjin Qing,Changbin Sun,Zhangbao Huang, Jianlong Pan,Liang Wang State key laboratory for Conservation and Utilization of Subtropical Agro-bioresources,key laboratory of ministry of education for microbial and plant genetic engineering,College of Life Science and Technology,Guangxi University,Guangxi 530004

Maize is the third largest planted cereal crops in the ***,its production is usually affected greatly by extreme environmental conditions imposed by stresses of abiotic factors such as drought,salt,and aluminium(A1) *** is very difficult to breed maize varieties of high stress tolerance through the conventional breeding strategies because these traits result from polygenic effects and in part due to lack in credible traits for breeding and *** stress tolerance is controlled mainly at the transcriptional ***,understanding of gene

关键词： Maize abiotic stress removal of the stress transcriptomics

利用有序差异显示技术克隆受稻瘟病菌诱导表达的水稻cDNA的研究

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生物化学与生物物理进展 2004年第7期31卷 635-642页

作者：罗文永李晓方胡骏刘文华肖昕刘良式中山大学植物基因工程教育部重点实验室广州510275 广东省农业科学院水稻研究所

利用有序差异显示技术 (ODD)分析受稻瘟病菌诱导表达的水稻基因 ,通过反式RNA印迹进行辅助筛选 ,获得了 37个在接种稻瘟病菌后表达量增强的水稻cDNA克隆 .采用RNA印迹对其中 5个克隆在接种稻瘟病菌后的表达分析表明 ,这些克隆在抗病以... 详细信息

利用有序差异显示技术 (ODD)分析受稻瘟病菌诱导表达的水稻基因 ,通过反式RNA印迹进行辅助筛选 ,获得了 37个在接种稻瘟病菌后表达量增强的水稻cDNA克隆 .采用RNA印迹对其中 5个克隆在接种稻瘟病菌后的表达分析表明 ,这些克隆在抗病以及感病的水稻株系中都具有诱导表达的特点 .根据序列同源性分析 ,与这些克隆序列相应的同源基因可能涉及抑制病原菌生长、清除真菌毒素、传递抗病信号以及调节宿主生理状态等几方面的功能 .

关键词：有序差异显示技术(ODD) 水稻稻瘟病菌 cDNA 克隆

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Isolation and Functional Characterization of a Gene Encoding Cyclase from Wheat

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Isolation and Functional Characterization of a Gene Encoding...

第十二届全国植物基因组学大会

作者： Cheng Wang,Jian Zeng,Cheng Ma,Yin Li,Yuesheng Wang,Guangxiao Yang,Guangyuan He~* Chinauk Hustrres genetic engineering and Genomics Joint {2.,The genetic engineering International Cooperation Base of ministry of Science and Technology,The key {2. of Molecular Biophysics of ministry of education,College of Life Science,Wuhan 430074,China

Wheat endosperm color is largely determined by the presence of carotenoid *** conversion of lycopeneβ-*** present(β-carotene(yellow) is catalysed by lycopene((red) to study describes the cloning and functional characterization of a gene encodingβ-cyclase from wheat(*** *** Spring) by the rapid(lycopene amplification of cDNA ends(RACE) *** cDNA sequence of Taβ-lcy was 1884-bp long,containing an ORF sequence of 1455 bp,and encodes a putative protein of 484 amino acid residues with an estimated relative molecular mass of 53.2. *** analysis showed that Taβ-lcy was highly homologous with lycopeneβ-cyclase of Zea may and that in other higher plant

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The effect of decreasing alkalinity on microbial community dynamics in a sulfate-reducing bioreactor as analyzed by PCR-SSCP

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Science China(Life Sciences) 2006年第4期49卷 370-378页

作者： REN Nanqi1, ZHAO Yangguo1, WANG Aijie1, GAO Chongyang2. SHANG Huaixiang1, LIU Yiwei1 & WAN Chunli1 1. School of Environmental and Municipal engineering, Harbin Institute of Technology, Harbin 150090, China 2. key {2. of Forest plant Ecology, ministry of education, Northeast Forestry University, Harbin 150040, China 1. School of Environmental and Municipal engineering Harbin Institute of Technology Harbin 150090 China 2. key laboratory of Forest {2. Ecology Ministry of Education Northeast Forestry University Harbin 150040 China

PCR-single-strand conformation polymorphism (SSCP) and Southern blotting tech-niques were adopted to investigate microbial community dynamics in a sulfate-reducing bioreactor caused by decreasing influent alkalinity. Experimental results indicated that the sulfate-removal rate approached 87% in 2. d under the conditions of influent alkalinity of 4000 mg/L (as CaCO3) and sul-fate-loading rate of 4.8 g/(L·d), which indicated that the bioreactor started up successfully. The analy-sis of microbial community structure in this stage showed that Lactococcus sp., Anaerofilum sp. and Kluyvera sp. were dominant populations. It was found that when influent alkalinity reduced to 1000 mg/L, sulfate-removal rate decreased rapidly to 35% in 3 d. Then influent alkalinity was increased to 3000 mg/L, the sulfate-removal rate rose to 55%. Under these conditions, the populations of Dysgo-nomonas sp., Sporobacte sp., Obesumbacterium sp. and Clostridium sp. got to rich, which predomi-nated in the community together with Lactococcus sp., Anaerofilum sp. and Kluyvera sp. However, when the alkalinity was decreased to 1500 mg/L, the sulfate-removal rate rose to and kept stable at 70% and populations of Dysgonomonas sp., Sporobacter sp. and Obesumbacterium sp. died out, while some strains of Desulfovibrio sp. and Clostridium sp. increased in concentration. In order to determine the minimum alkalinity value that the system could tolerate, the influent alkalinity was de-creased from 1500 to 400 mg/L secondly. This resulted in the sulfate-removal rate, pH value and ef-fluent alkalinity dropping quickly. The amount of Petrotoga sp., Prevotella sp., Kluyvera sp. and Neisseria sp. reduced obviously. The result data from Southern blotting indicated that the amount of sulfate-reducing bacteria (SRBs) decreased with influent alkalinity dropping. Analysis of the microbial community structure and diversity showed that the SRBs populations were very abundant in the in-oculated activated sludge and the al

关键词： alkalinity, community dynamics, PCR-single-strand conformation polymorphism (SSCP), Southern blotting, sulfate-reducing bacterium (SRB), fermentative acidogenic bacterium (FAB).

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Cloning and Characterization of CBL Genes in Wheat(Triticum aestivum L.)

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Cloning and Characterization of CBL Genes in Wheat(Triticum ...

第十二届全国植物基因组学大会

作者： Yi Zhou,Lang Xu,Tao Sun,Guangyuan He~*,Guangxiao Yang~* China-UK HUST-RRes genetic engineering and Genomics Joint {2.,The genetic engineering International Cooperation Base of ministry of Science and Technology,The key {2. of Molecular Biophysics of ministry of education,College of Life Science and Technology,Huazhong University of Science & Technology,Wuhan 430074,China

Abiotic stresses,including salinity,drough and low or high temperature are important causes of crop loss *** adapt to the adverse abiotic stresses,plants can initiate a number of molecular,cellular and physiological changes and then activate cascades of molecular net ***-binding proteins,like Calcineurin B-like(CBL) proteins have been implicated as important Ca sensors in plant-specific calcium *** proteins recognize specific calcium signatures and form a complex network with their target kinases named CBL-interacting protein kinases(CIPKs).Based on sequence similarity,several ESTs se-

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Detecting Antibacterial Activities of Puroindoline A Mutants Using Fluorescence Microscopy and Statistical Analysis in vitro

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Detecting Antibacterial Activities of Puroindoline A Mutants...

第十二届全国植物基因组学大会

作者： QianZheng,Yingjie Miao,Jiutong Sun,Guangxiao Yang,Guangyuan He~* China-UK HUST-RRes genetic engineering and Genomics Joint {2.,The genetic engineering International Cooperation Base of ministry of Science and Technology,The key {2. of Molecular Biophysics of ministry of education,College of Life Science and Technology,Huazhong University of Science & Technology,Wuhan 430074,China

The puroindoline(Pin) genes,including Pina and Pinb localizing on the short arm of 5D chromosome in hexaploid wheat,encode two endosperm-specific proteins known as PINA and PINB,*** and PINB are unique in structure in having a tryptophan-rich domain(TRD) and play key roles in determining the wheat grain texture hardness,as well as defending against plant *** was observed to have much greater membrane-binding property and anti{2. activity than PINB due to the more Trp residues in the unique TRD *** is of interest whether the anti{2. activity of PINA is enhanced if additional

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Knob-associated tandem repeats on mitotic chromosomes in Zea diploperennis, maize and theirs hybrids

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Knob-associated tandem repeats on mitotic chromosomes in Zea...

湖北省遗传学会第七次代表大会暨学术讨论会

作者： XIONG Zhiyong~(1.2., GAO Yuan~1, HE Guanyuan~2. GU Mingguang~3, GUO Lequn~3 & SONG Yunchun~(1*) (1. key Lab of the ministry of {2. for plant Developmental Biology, Wuhan University, Wuhan 430072. China 2. China-UK HUST-RRes Crop genetic engineering and Genomics Joint laboratory, Huazhong University of Science and Technology, Wuhan 430074, China 3. Institute of genetics, The Chinese Academy of Sciences, Beijing 1000101, China)

Knob-associated tandem repeats, 180-bp repeats and TR-1 elements, together with 45S rDNA were located on mitotic chromosomes of Zea diploperennis (DP), maize inbred line F102.and theirs hybrid. In DP, knobs on chromosomes 1S, 4S, 4L and 5S are composed predominantly of the 180-bp repeats. In addition, 180-bp repeats co-exited with TR-1 elements were also detected in other seven sites on six chromosome pairs. In maize inbred line F102. 180-bp repeats were present in chromosomes 7S and one homologue of chromosomes 8L. TR-1 elements appeared on satellite of chromosome 6 and no detectable hybridization site co-located with 180-bp repeats was observed in maize F102. Polymorphism of size, number, and distribution of 180-bp repeats and TR-1 were revealed among different chromosomes and between homologous chromosomes in these two species. Using these excellent landmarks, the interspecific hybrid of maize and DP were identified. The results presented suggestthat comparative analysis of 180-bp repeats and TR-1 elements may aid in understanding the genome organization and the evolution in Zea.

关键词： Zea mays Zea diploperennis repetitive DNA sequence fluorescence in situ hybridization heterochromatic knob.