Detecting Antibacterial Activities of Puroindoline A Mutants Using Fluorescence Microscopy and Statistical Analysis in vitro

作     者：QianZheng,Yingjie Miao,Jiutong Sun,Guangxiao Yang,Guangyuan He~* China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory,The Genetic Engineering International Cooperation Base of Ministry of Science and Technology,The Key Laboratory of Molecular Biophysics of Ministry of Education,College of Life Science and Technology,Huazhong University of Science & Technology,Wuhan 430074,China 

会议名称：《第十二届全国植物基因组学大会》

会议日期：2011年

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

摘      要：The puroindoline(Pin) genes,including Pina and Pinb localizing on the short arm of 5D chromosome in hexaploid wheat,encode two endosperm-specific proteins known as PINA and PINB,*** and PINB are unique in structure in having a tryptophan-rich domain(TRD) and play key roles in determining the wheat grain texture hardness,as well as defending against plant *** was observed to have much greater membrane-binding property and antimicrobial activity than PINB due to the more Trp residues in the unique TRD *** is of interest whether the antimicrobial activity of PINA is enhanced if additional