Cytogenetic comparisons between A and G genomes in Oryza using genomic in situ hybridization

作     者：Zhi Yong Xiong~(1,2) Guang Xuan Tan~1 Guang Yuan He~2 Guang Cun He~1 Yun Chun Song~11 Key Laboratoty of the Ministry of Education for Plant Developmental Biology,College of Life Sciences,Wuhan University,Wuhan 430072,China.2 China-UK HUST-R Res Genetic Engineering and Genomics Joint Laboratory,Huazhong University of Science and Technology,Wuhan 430074,China Zhi Yong Xiong~(1,2) Guang Xuan Tan~1 Guang Yuan He~2 Guang Cun He~1 Yun Chun Song~11 Key Laboratoty of the Ministry of Education for Plant Developmental Biology,College of Life Sciences,Wuhan University,Wuhan 430072,China.2 China-UK HUST-R Res Genetic Engineering and Genomics Joint Laboratory,Huazhong University of Science and Technology,Wuhan 430074,China

作者机构：KeyLaboratoryoftheMinistryofEducationforPlantDevelopmentalBiologyCollegeofLifeSciencesWuhanUniversityWuhan430072China China-UKHUST-RResGeneticEngineeringandGenomicsJointLaboratoryHuazhongUniversityofScience 

出 版 物：《Cell Research》 (细胞研究(英文版))

年 卷 期：2006年第16卷第3期

页      面：260-266页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

主　　题：Oryza sativa Oryza meyeriana 45S rDNA genomic in situ hybridization 

摘      要：The genomic structures ofOryza sativa （A genome） and *** （G genome） were comparatively studied usingbieolor genomic in situ hybridization （GISH）.GISH was clearly able to discriminate between the chromosomes of *** and *** in the interspecific F1 hybrids without blocking DNA,and co-hybridization was hardly *** average mitotic chromosome length of *** was found to be 1.69 times that of ***.A comparison of4,6-diamidino-2-phenylindole staining showed that the chromosomes of *** were more extensively labelled,suggesting that the G genome is amplified with more repetitive sequences than the A *** interphase nuclei,9-12chromocenters were normally detected and nearly all the chromocenters constituted the G genome-specific *** larger chromocenters formed by chromatin compaction corresponding to the G genome were detected in the hybridcompared with its *** pachytene of the F1 hybrid,most chromosomes of A and G did not synapse eachother except tot 1-2 chromosomes paired at the end of their *** meiotic metaphase I,three types of chromosomalassociations,i.e.O,sativa-O,sotiva（A-A）,***-O,meyeriana （A-G） and ***-O,meyeriana （G-G）,wereobserved in the F1 *** A-G chromosome pairing configurations included bivalcnts and *** resultsprovided a foundation toward studying genome organization and evolution of ***.