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An analysis on the evolvement processes of lake eutrophication and their characteristics of the typical lakes in the middle and lower reaches of yangtze River

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Chinese Science Bulletin 2006年第13期51卷 1603-1613页

作者： CHENG Xiaoying1,2.3.& LI Shijie1 1. Nanjing Institute of Geography & Limnology, Chinese Academy of Sciences, Nanjing 2.0008, China 2. the key laboratory of industrial biotechnology,{3.,{2.,{3., China 3. School of biotechnology,{2.,{3., China Nanjing Institute of Geography & Limnology Chinese Academy of Sciences Nanjing China The key laboratory of {2. {3. Ministry of Education Southern Yangtze University Wuxi China School of biotechnology Southern Yangtze University Wuxi China

According to the study on the aquatic environment of the typical lakes in the middle and lower reaches of yangtze River, lake eutrophication is a process in which the lake ecosystem degenerates, resulting in the deterioration of the water quality due to the enrichment of the nutrients. Moreover, the succession of lake ecosystem and change of water quality both couple well with the evolvement of tro- phic state. Then the conceptual model is mentioned and ten lake states are classified. Based on these, the authors analyzed the eutrophication processes of the typical lakes in the middle and lower reaches of yangtze River. The large lakes with taking in - send- ing out water typical of Poyang Lake evolve from State 1 before the 1980s into State 2.after the 1980s, the large and medium lakes typical of Taihu Lake evolve from State 1-2.in the 1960s into State 8 in the 1980s then into State 9 in the 1990s, the medium and small shallow lakes typical of Honghu Lake evolve from State 1 in the 1960s into State 2.after the 1980s and the small city (suburban) lakes typical of Donghu Lake evolve from State 2.in the 1960s into State 9 after the 1970s. Further, the 1980s is the key con- version period of the lakes of various types typical of Poyang Lake, Honghu Lake and Taihu Lake con- verting into mesotrophic state, the 1990s is that of Taihu Lake into eutrophic state and the 1970s is thatof the small city (suburban) lakes typical of Donghu Lake into eutrophic state. The series of nutrient evolvement of different typical lakes are similar, but the processes are not synchronous, which is related to the various human and natural driving mechanisms in lake basins.

关键词：长江湖泊富营养化概念模型转变时期驱动机制

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Study on the electric double layer of a cylindrical reverse micelle with functional theoretical approach

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Science China Chemistry 2006年第3期49卷 219-224页

作者： WANG Zhengwu1,2. GUO Baomin2. ZHANG Gexin1 & YU Huixin1 1. key {3. of {2. biotechnology of {3. of education, southern yangtze university, wuxi 214.3., China 2. School of Chemical and Material Engineering, southern yangtze university, wuxi 214.3., China 1. key laboratory of {2. {3. of {3. of education Southern Yangtze University Wuxi 214036 China 2. School of Chemical and Material Engineering Southern Yangtze University Wuxi 214036 China

The iterative method in functional analysis is applied to looking for asolution of the Poisson-Boltzmann equation in order to describe the problems of the distribution ofthe potentials in the electric double layer (EDL) inside the water pool for a cylindrical inversemicelle. Potentials as a function of the position of a particular point in EDL are computed, whichdisplay a quantitative agreement with those from earlier calculation of Debye and Huckel in the caseof low potentials. But it is also shown that in the higher-potential range the iterativecalculations can give more accurate results. These results indicate the utility of this functionalanalysis technique in the description of the properties of EDL for a cylindrical inverse micelle.

关键词： cylindrical reverse micelle, electrostatic double layer, Poisson-Boltzmann equation, functional analytic theory.

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Partial Rescue of pos5 Mutants by YEF1 and UTR1 Genes in Saccharomyces cerevisiae

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Acta Biochimica et Biophysica Sinica 2006年第5期38卷 293-298页

作者： Yong-Fu LI~1 Feng SHI~(2.)1 School of Food Science and Technology2.The key {3. of {2. biotechnology,{3. of education,southern yangtze university.wuxi {13.,China School of Food Science and Technology and2 The Key Laboratory of Industrial BiotechnologyMinistry of EducationSouthern Yangtze UniversityWuxi 214036China

Three NAD kinase homologs,encoded by UTR1,POS5 and YEF1 genes,are found in the yeastSaccharomyces cerevisiae and proven to be important sources of NADPH for the ***5p,existing in themitochondrial matrix,is critical for higher temperature endurance and mitochondrial functions,such asglycerol usability and arginine *** constructing the high-copy expression plasmids ofYEF1 and UTR1,which contained the green fluorescent protein reporter tag at their 3. terminus,and introducingthem into POS5 gene deletion mutants(***5,utr1pos5,yef1pos5 and utr1yef1pos5),the high-copy YEF1and UTR1 plasmids carrying transformants for pos5 mutants were *** temperature sensitivityand growth phenotype on media with glycerol as the sole carbon source,or on media without arginine,*** showed the partial rescue of mitochondrial dysfunctions and temperature sensitivity ofpos5 mutants by the high-copy YEF1 gene,and of glycerol growth defect and temperature sensitivity by thehigh-copy UTR1 gene,which confirmed the potential supplying ability of Yeflp and Utrlp for mitochondrialNADP(H)and implied the weak transport of NADP from cytosol to ***,even throughthe green fluorescent protein reporter label,the subcellular localization of Yef1p and Utr1p in yeast cells couldnot be observed,which indicated the low expression level of these two NAD kinase homologs.

关键词： NAD kinase Saccharomyces cerevisiae mitochondrial function temperature sensitivity subcellular localization

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Analysis of the Resveratrol-binding Protein using Phage-displayed Random Peptide Library

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Acta Biochimica et Biophysica Sinica 2006年第5期38卷 342-348页

作者： Lei FENG Jian JIN Lian-Feng ZHANG Ting YAN Wen-Yi TAO The key laboratory of {2. {3.,{3. of education,southern yangtze university,wuxi 214.3.,China Thekeylaboratoryof{2.{3. MinistryofEducationSouthernYangtzeUniversityWuxi214036China

Resveratrol,a plant polyphenol,is found in significant amounts in the skin of grapes and insome traditional *** is reported to exert different biological activities,such as inhibiting lipid peroxidation,scavenging free radicals,inhibiting platelet aggregation,and anticancer *** order to screen theresveratrol-binding proteins,we synthesized biotinylated resveratrol,purified by liquid chromatography andimmobilized it into streptavidin-coated microplate wells.3.(4,5-Demethylthiazol-)-{3.5-diphenyl tetrazoliumbromide assay showed little change in the anticancer activity of biotinylated resveratrol in vitro.A randomlibrary of phage-displayed peptides was screened for binding to immobilized resveratrol to isolate resveratrol-binding *** peptides were found to bind to resveratrol specifically,which was proven byenzyme-linked immunosorbent *** amino acid sequence analysis of the selected peptides andhuman proteins using the BLAST program,the results showed that resveratrol has an affinity for variousproteins such as breast cancer-associated antigen,breast cancer resistance protein,death-associatedtranscription factor,and human cyclin-dependent *** results demonstrate that our study providesa feasible method for the study of binding proteins of natural compounds using a phage-displayed randompeptide library.

关键词： resveratrol binding protein phage display anticancer activity

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一株嗜热子囊菌产生的碱性耐热过氧化氢酶及其应用潜力

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生物工程学报 2004年第3期20卷 423-428页

作者：方芳李寅堵国成张娟陈坚江南大学工业生物技术教育部重点实验室江南大学生物工程学院环境生物技术研究室无锡214036

研究了一株嗜热子囊菌产过氧化氢酶的摇瓶发酵条件 ,并对其在纺织工业中的应用潜力进行了评价。以2.0g L糊精和 1% (V V)乙醇为混合碳源时 ,过氧化氢酶酶活达到 15 94u mL ,比以糊精和乙醇单独为碳源时过氧化氢酶的活力之和还高 2.3.。... 详细信息

研究了一株嗜热子囊菌产过氧化氢酶的摇瓶发酵条件 ,并对其在纺织工业中的应用潜力进行了评价。以2.0g L糊精和 1% (V V)乙醇为混合碳源时 ,过氧化氢酶酶活达到 15 94u mL ,比以糊精和乙醇单独为碳源时过氧化氢酶的活力之和还高 2.3.。改变培养基的初始pH、提高发酵液中的溶氧水平及添加外源过氧化氢 ,过氧化氢酶的产量进一步提高到 2.76 2. mL ,比优化前提高了 5 8倍。将嗜热子囊菌的过氧化氢酶同来源于牛肝、黑曲霉的过氧化氢酶进行了热 (70℃ ,80℃ ,90℃ )、碱 (pH 9 0 ,pH 10 0 ,pH 11 0 )稳定性的比较。结果显示 ,产自嗜热子囊菌的过氧化氢酶对高温和强碱性的耐受性能明显优于其它来源的酶 ,在纺织染整工艺中具有良好的应用潜力。

关键词：过氧化氢酶嗜热子囊菌混合碳源过氧化氢热、碱稳定性

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分子对接与全局极小化方法

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生物化学与生物物理进展 2001年第3期28卷 314-317页

作者：李炜疆江南大学工业生物技术教育部重点实验室无锡214036

全局极小化方法及其在结构生物学中的应用近年来取得了显著的进展 .适当简化的分子对接问题是全局极小化方法的一个很好目标 ,并且是当前一个相当活跃的研究领域 .对接可分为两类 :主要用于从头配体设计的细致对接和用于已知化合物数据... 详细信息

全局极小化方法及其在结构生物学中的应用近年来取得了显著的进展 .适当简化的分子对接问题是全局极小化方法的一个很好目标 ,并且是当前一个相当活跃的研究领域 .对接可分为两类 :主要用于从头配体设计的细致对接和用于已知化合物数据库筛选以发现药物的粗略对接 ,它们对全局极小化算法的要求是不同的 .简要评述了新出现的适合于对接问题的随机和确定性全局极小化算法 ,其中势能平滑算法看来很有希望 ,值得密切关注 .

关键词：分子对接全局极小化势能平滑

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溶氧及pH对产朊假丝酵母分批发酵生产谷胱甘肽的影响

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生物工程学报 2003年第6期19卷 734-739页

作者：卫功元李寅堵国成陈坚江南大学工业生物技术教育部重点实验室生物工程学院环境生物技术研究室无锡214036

在 7L发酵罐中研究了溶氧和pH对产朊假丝酵母分批发酵生产谷胱甘肽的影响。结果表明 ,当葡萄糖浓度为 3.g L且通气量控制在 5L min时 ,搅拌转速达到 3. 0r min即可满足细胞生长和谷胱甘肽合成对溶解氧的需求。不同pH控制方式对谷胱甘肽... 详细信息

在 7L发酵罐中研究了溶氧和pH对产朊假丝酵母分批发酵生产谷胱甘肽的影响。结果表明 ,当葡萄糖浓度为 3.g L且通气量控制在 5L min时 ,搅拌转速达到 3. 0r min即可满足细胞生长和谷胱甘肽合成对溶解氧的需求。不同pH控制方式对谷胱甘肽分批发酵的影响有较大差异。不控制pH时 ,细胞干重和谷胱甘肽产量比控制pH为 5 5的发酵分别低 2.7%和 95 % ,且有 5 0 %的谷胱甘肽向胞外渗漏。研究了将pH控制在 4 0、4 5、5 0、5 5、6 0和 6 5的谷胱甘肽分批发酵过程 ,发现在pH 5 5时谷胱甘肽总产量最高。用前期研究建立的动力学模型模拟了不同pH (4 0～ 6 5 )下的分批发酵过程 ,并从动力学角度解释了pH对细胞生长和谷胱甘肽合成的影响。

关键词：谷胱甘肽分批发酵产朊假丝酵母溶氧 pH

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La(Ⅲ)对高粱种子萌发的影响

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土壤通报 2009年第3期40卷 653-655页

作者：邱琳周青江南大学工业生物技术教育部重点实验室江苏无锡214222

以高粱(Sorghum vulare")熊杂9号"为试材,采用不同浓度LaCl3.液浸种,研究稀土元素镧对其萌发过程的影响.。实验结果表明,5~40mgL-1LaCl3.够提高高粱种子的发芽率(0.1%~8.7%)、发芽势(1.6%~10%)、发芽指数(2.0%~16.7%)、活力指数(9.1%~... 详细信息

以高粱(Sorghum vulare")熊杂9号"为试材,采用不同浓度LaCl3.液浸种,研究稀土元素镧对其萌发过程的影响.。实验结果表明,5~40mgL-1LaCl3.够提高高粱种子的发芽率(0.1%~8.7%)、发芽势(1.6%~10%)、发芽指数(2.0%~16.7%)、活力指数(9.1%~3..7%)、贮藏物质运转率(0.7%~4.0%)、贮藏物质消耗率(0.8%~6.0%)和芽长(-11.6%^-17.4%)及根长抑制指数(-3.3.^-2..9%)。10mgL-1LaCl3.影响高粱种子萌发指标的显效剂量。

关键词：稀土镧高粱种子萌发

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Preparation and characterization of sponge-like composites by cross-linking hyaluronic acid and carboxymethylcellulose sodium with adipic dihydrazide

Preparation and characterization of sponge-like composites b...

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第三届全国化学工程与生物化工年会

作者： Long Liu , Dengru Liu, Guocheng Du, Jian Chen School of biotechnology, southern yangtze university, {2., {3., China key laboratory of industrial biotechnology, ministry of education, southern yangtze university, {2., {3., China

＜正＞In this study, sponge-like composites were prepared by cross-linking different amounts of hyaluronic acid （HA） and carboxymethylcellulose sodium （CMC-Na）. Adipic dihydrazide （ADH） was employed as the cross-linker and water-soluble 1-ethyl-3.[3.（dimethylaminopropyl）] carbodiimide （EDC） as the carboxyl-activating agent. Fourier-Transform Infrared （FT-IR） analysis was performed to characterize the expected amide linkages in the cross-linked composites, and the scanning electron microscopic （SEM） analysis was carried out to view their microstructures. Furthermore, the physico-chemical properties, such as swelling property, thermostability, the resistance to hyaluronidase （HAse） degradation and the antioxidant abilities towards hydroxyl free radical （OH）, were characterized. The FT-IR spectra showed the amide peak at 1476.11 cm-1, indicating the cross-linking between HA and CMC-Na by ADH. Scanning electron microscopic （SEM） views demonstrated that the microstructures of the composites were dependent on HA content in the composites. The equilibrium swelling ratio was about 2., indicating the excellent water-uptake abilities of the composites. The HA-CMCNa composites showed a thermal stability up to 2.7℃. When exposed to PBS solution containing HAse （100 U/ml）, all of the composites could be degraded quickly in 15 h. But if the concentration of ADH and EDC in the cross-linking reaction system was increased up to 10 mmol/L respectively, the degradation process would last for 60 h. The prepared composites possessed great antioxidant abilities against ·OH and the ability to scavenge ·OH depended on the composition. All these results showed that the prepared HA-CMCNa composites were a kind of novel HA derivatives and have great potentials in biomedical application.

关键词： Hyaluronic acid (HA) Carboxymethylcellulose sodium (CMCNa) cross-linking

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真氧产碱杆菌在双营养(碳、氮)限制区内合成聚羟基烷酸酯

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生物工程学报 2003年第4期19卷 497-501页

作者：严群堵国成陈坚江南大学工业生物技术教育部重点实验室无锡214036

研究了真氧产碱杆菌以混合有机酸为碳源 ,硫酸铵为氮源 ,在双营养 (碳、氮 )限制区内聚羟基烷酸酯的生物合成。结果表明 :双营养限制区的长度与聚羟基烷酸酯的产量呈正相关。同时 ,在对两种不同的双营养限制区实现方式进行比较后发现 ,... 详细信息

研究了真氧产碱杆菌以混合有机酸为碳源 ,硫酸铵为氮源 ,在双营养 (碳、氮 )限制区内聚羟基烷酸酯的生物合成。结果表明 :双营养限制区的长度与聚羟基烷酸酯的产量呈正相关。同时 ,在对两种不同的双营养限制区实现方式进行比较后发现 ,首先限制碳源的双营养限制方式比首先限制氮源的双营养限制方式更有利于聚羟基烷酸酯的合成 ;在这两种不同营养限制方式下 ,PHAs的最高产量分别为 3. 72. L和 2. 5 5g L。

关键词：真氧产碱杆菌双营养限制区聚羟基烷酸酯生物合成

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