Partial Rescue of pos5 Mutants by YEF1 and UTR1 Genes in Saccharomyces cerevisiae

作     者：Yong-Fu LI~1 Feng SHI~(2*)1 School of Food Science and Technology2 The Key Laboratory of Industrial Biotechnology,Ministry of Education,Southern Yangtze University.Wuxi 214036,China 

作者机构：School of Food Science and Technology Ministry of Education Southern Yangtze UniversityWuxi 214036 China The Key Laboratory of Industrial Biotechnology Ministry of Education Southern Yangtze UniversityWuxi 214036 China 

出 版 物：《Acta Biochimica et Biophysica Sinica》 (生物化学与生物物理学报(英文版))

年 卷 期：2006年第38卷第5期

页      面：293-298页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071007[理学-遗传学] 

主　　题：NAD kinase Saccharomyces cerevisiae mitochondrial function temperature sensitivity subcellular localization 

摘      要：Three NAD kinase homologs,encoded by UTR1,POS5 and YEF1 genes,are found in the yeastSaccharomyces cerevisiae and proven to be important sources of NADPH for the ***5p,existing in themitochondrial matrix,is critical for higher temperature endurance and mitochondrial functions,such asglycerol usability and arginine *** constructing the high-copy expression plasmids ofYEF1 and UTR1,which contained the green fluorescent protein reporter tag at their 3’ terminus,and introducingthem into POS5 gene deletion mutants(***5,utr1pos5,yef1pos5 and utr1yef1pos5),the high-copy YEF1and UTR1 plasmids carrying transformants for pos5 mutants were *** temperature sensitivityand growth phenotype on media with glycerol as the sole carbon source,or on media without arginine,*** showed the partial rescue of mitochondrial dysfunctions and temperature sensitivity ofpos5 mutants by the high-copy YEF1 gene,and of glycerol growth defect and temperature sensitivity by thehigh-copy UTR1 gene,which confirmed the potential supplying ability of Yeflp and Utrlp for mitochondrialNADP(H)and implied the weak transport of NADP from cytosol to ***,even throughthe green fluorescent protein reporter label,the subcellular localization of Yef1p and Utr1p in yeast cells couldnot be observed,which indicated the low expression level of these two NAD kinase homologs.