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Formation of complexes of Rubisco-Rubisco activase from La^3+, Ce^3+ treatment spinach

science china Chemistry 2005年第1期48卷 67-74页

作者： HONG Fashui1, LIU Chao1, ZHENG Lei1, WANG Xuefeng1, WU Kang1, SONG Weiping1, Lü Shipeng1, TAO Ye2.& ZHAO Guiwen3 1. College of Life sciences, Suzhou Universty, Suzhou 2.5006, china {2., institute of high energy physics, the chinese academy of science, beijing 100039, china 3. Chemical Department, University of science and Technology of china, Hefei 2.002., china 1. College of Life sciences Suzhou Universty 215006 Suzhou China 2. synchrotron radiation laboratory Institute of High Energy Physics The Chinese Academy of Science 100039 Beijing China 3. Chemical Department University of Science and Technology of China 230026 Hefei China

The complex of Rubisco and Rubisco activase from LaCl3-, CeCl3- treated spinach in vivo is induced. SDS-PAGE result shows that the purified proteins from LaCl3-, CeCl3- treated spinach have not only large and small subunits (55 kD, 14.4 kD) of Rubisco, but also two large subunits of 45 kD and 41 kD near the large subunits of Rubisco. Native-PAGE shows that the purified proteins from LaCl3-, CeCl3- treated spinach have not only a band of Rubisco (560 kD), but also a band of about 1100 kD, about twice distant from Rubisco, which might be a complex of Rubisco and Rubisco activase. The purified enzyme activities from LaCl3-, CeCl3-treated spinach are 1.8 and 2.8 times that of the control,the intensities of absorption and fluorescence are sig- nificantly higher than that of the purified Rubisco from the control, and the total sulfhydryl groups and available sulfhydryl groups are 36—39 –SH per mol enzyme, 14—2. –SH per mol enzyme more than those of the purified Rubisco from the control, respectively. The CD spectra show that the secondary structure of the purified enzyme from LaCl3-, CeCl3 -treated spinach is very dif- ferent from the control. The enzyme activities from LaCl3-, CeCl3- treated spinach in vivo are 1.5 and 1.9 times those of the control.

关键词： La3+, Ce3+, spinach, Rubisco, complex of Rubisco and Rubisco acitivase, spectral characteristics.

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Compression behavior of Zr_(41)Ti_(14)Cu_(12.5)Ni_(10)Be_(2..5) bulk metallic glass up to 2. GPa

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science china({2.,Mechanics & Astronomy) 2005年第3期48卷 319-324页

作者： LI Gong 1, 2., ZHAN Zaiji 2., LIU Jing 3 , YUN Jinku 1 , SUN Liling 2., LIU Riping 1 , ZHANG Jun 2.& WANG Wenkui 1, 2.1. Key laboratory of Metastable Materials science and Technology, Yanshan University, Qinhuangdao 066004,{2. 2. Center for Condensed Matter physics, institute of physics, chinese academy of sciences, beijing 100080,{2. 3. BSRF, institute of high energy physics, chinese academy of sciences, beijing 100039,{2. 1. Key laboratory of Metastable Materials science and Technology Yanshan University 066004 Qinhuangdao China 2. Center for Condensed Matter physics Institute of Physics Chinese Academy of Sciences 100080 Beijing China 3. BSRF Institute of High Energy Physics Chinese Academy of Sciences 100039 Beijing China

The compression of a Zr41Ti14Cu12.5Ni10Be2..5 bulk metallic glass (BMG) is investigated at room temperature up to 2. GPa using in-situ high pressure energy dispersive X-ray diffraction with a {2. radiation source. The pressure-induced structural relaxation is exhibited. It is found that below about 8 GPa, the existence of excess free volume contributes to the rapid structural relaxation, which gives rise to the rapid volumetric change, and the structural relaxation results in the structural stiffness under higher pressure.

关键词： bulk metallic glass, high pressure, structural relaxation, synchrotron radiation.

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Studies on BEPC upgrade from pretzel to double-ring

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science china({2.,Mechanics & Astronomy) 2010年第11期53卷 2084-2089页

作者： ZHANG Chuang institute of high energy physics,chinese {2. of sciences,beijing 100049,china 1. institute of high energy physics Chinese Academy of Sciences Beijing 100049 China

physics opportunity in the charm and τ energy region calls for {2.er luminosity of the beijing Electron-Positron Collider (BEPC).The BEPC has experienced continuous improvement since its completion in ***,as a major upgrading project of the BEPC,aims at enhancing its luminosity of factor 30–100,following the principle of "one machine,two purposes" as adopted in BEPC,so that both {2. energy physics and synchrotron radiation experiments can be carried out with *** the BEPC to BEPCII,the double-ring scheme with multi-bunch e +-e-collision in the large crossing angle was studied in comparison with the single-ring pretzel *** the dedicated synchrotron radiation operation,artful "bridges" are designed to connect two halves of the outer ring in both the southern and the northern crossing *** paper reviews the studies on the BEPC upgrade from pretzel to double-ring structure of BEPCII.

关键词： collider storage ring high energy physics synchrotron radiation

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INVESTIGATION ON THE VALENCE STATE OF Ce ATOMIN BULK AND NANOCRYSTAL CeO_2.BY X-RAY ABSORPTION ANDPHOTOEMISSION

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Acta Metallurgica Sinica(English Letters) 2001年第6期14卷 511-516页

作者： K. Ibrahim, Z.Y. Wu, J. Zhang, M.I. Abbas, F.Q. Liu and H.J. Qian (beijing synchrotron {2. laboratory, institute of high energy physics, the chinese academy of sciences, P.O. Box 918, beijing 100039, china) beijing synchrotron Radiat. Lab. Inst. of High Energy Phys. Chinese Acad. of Sci. P.O. Box 918 Beijing 100039 China

Valence band photoemission spectra (PES) for both bulk and nanocrystal CeO2.have been measured on and off resonance of Ce 4d-4f absorption edge. The PES show that the bulk and nanocrystal CeO2.of diameter ranging from 8nm to 50nm exhibit a peak near Fermi edge with binding energy of about 1.8eV. The 1.8eV peak shows a strong dependence on excitation energy, although it looks like the contribution of Ce3+ ion following the data reported in literatures. However, according to the results of resonance photoemission and X-ray absorption spectra at 1s edge, this electronic structure may be associated to the intermediate state charge transfer effects.

关键词： rare earth oxide photoemission electronic structure photoabsorption

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RESONANT PHOTOEMISSION OF BULK CeO_(2. AND NANO-CeO_(2. FILMS

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Acta Metallurgica Sinica(English Letters) 2001年第6期14卷 539-543页

作者： M.I. Abbas, K. Ibrahim, Z.Y. Wu, J. Zhang, F.Q. Liu and H.J. Qian (beijing synchrotron {2. laboratory, institute of high energy physics, the chinese academy of sciences, P.O. Box 918, beijing 100039, china) beijing synchrotron Radiat. Lab. Inst. of High Energy Phys. Chinese Acad. of Sci. P.O. Box 918 Beijing 100039 China

Photoemission behaviors of nano-CeO2.films with particle sizes ranging from 8 nm to 50 nm and bulk CeO2.in Ce 4 d-4 f absorption region have been investigated. Resonant enhancements of Ce 4 f valance band and Ce 5 p bands for nano film and bulk material have been observed. The variation of electron density of states in valance bands of nano and bulk structures of CeO2.is discussed in terms of Ce 4 d-4 f resonance.

关键词： Nanostructured materials Optical films Oxides Photoemission Resonance

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Distributions of arsenic and essential elements in pinna of arsenic hyperaccumulator Pteris vittata L.

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science china(Life sciences) 2005年第1期48卷 18-24页

作者： CHEN Tongbin, HUANG Zechun, HUANG Yuying & LEI Mei laboratory of Environmental Remediation, institute of Geographic {2.s and Natural Resources Research, chinese academy of {2.s, beijing 100101, china laboratory of synchrotron radiation, institute of high energy physics, chinese academy of {2.s, beijing 100039, china laboratory of Environmental Remediation Institute of Geographic Sciences and Natural Resources Research Chinese Academy of Sciences Beijing100101 China laboratory of synchrotron {2. Institute of High Energy Physics Chinese Academy of Sciences Beijing100039 China

The distributions of arsenic and 6 essential elements in the pinna of As hyperaccumulator, Pteris vittata L., were studied using synchrotron radiation X-ray fluorescence (SRXRF). Significant correlation between the distribution and mobility of the elements revealed that SRXRF study on the elemental distribution was feasible to inspect the transportations of elements in plants. The distribution of As in the pinna showed that As had great abilities to be transported in xylem vessels and from xylem to mesophyll. The distribution of K, one of the most mobile elements in plants, was similar to that of As, whereas the distributions of Fe and Ca with less mobility in plants were almost opposite to that of As in the pinna.

关键词： hyperaccumulator, chinese brake (Pteris vittata L.), arsenic, element, distribution, SRXRF.

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In situ study on dendrite growth of metallic alloy by a synchrotron radiation imaging technology

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science china(Technological sciences) 2010年第5期53卷 1278-1284页

作者： WANG TongMin1,XU JingJing1,LI Jun1,HUANG WanXia2.LIU ShengChu1 & LI TingJu1 1 School of Materials science and Engineering,Dalian University of Technology,Dalian 11602.,china 2.2.,chinese academy of sciences,beijing 100039,china 1. School of Materials science and Engineering Dalian University of Technology Dalian 116024 China2. Institute of High Energy Physics Chinese Academy of Sciences Beijing 100039 China

This study was trying to observe the real-time dendrite growth of Sn-Bi and Sn-Pb binary alloys by a synchrotron radiation imaging *** imaging system includes an intense and {2. brightness synchrotron radiation source,a {2.-resolution and fast-readout charge coupled device camera,an alloy sample and a Bridgman solidification *** imaging experiments were done at beijing synchrotron radiation Facility with an updated synchrotron radiation imaging technique,diffraction-enhanced imaging,which was firstly used to study the dendrite growth of metallic alloy.A series of growth behavior and morphology evolution of dendrite have been in situ observed,such as columnar-to-equiaxed transition,dendrite competition,dendrite fragmentation and floating,etc.,which can offer the direct proofs to verify or improve the solidification theories of metallic *** research opens a novel window for the study of alloy solidification and enables the unambiguous understanding of solidification processes in optically opaque,metallic alloys.

关键词： dendritic growth synchrotron radiation solidification diffraction-enhanced imaging metal and alloys

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Enhancement of magnetism of Zn0.95Co0.05O films by p-type Cu+ doping

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Progress in Natural science:Materials International 2011年第1期21卷 31-35页

作者： Wei-chang HAO~(1,5),Jian-jun LI~2,Huai-zhe XU~3,Jia-ou WANG~4,Tian-min WANG~1 1.Center of Materials physics and Chemistry,Beihang University,beijing 100083,china 2.Department of Materials science and Engineering, Anhui University of science and Technology,Huainan 232001,china 3.Department of physics,Beihang University,beijing 100083,china 4.beijing synchrotron radiation Facility,institute of high energy physics, chinese academy of sciences,100049,china 5.Key laboratory of Micro-nano Measurement,Manipulation and physics, Ministry of Education,Beihang University,beijing 100191,china [a] Center of Materials physics and Chemistry Beihang University Beijing 100083 China [b] Department of Materials science and Engineering Anhui University of Science and Technology Huainan 232001 China [c] Department of physics Beihang University Beijing 100083 China [d] beijing synchrotron {2. Facility Institute of High Energy Physics Chinese Academy of Sciences 100049 China [e] Key laboratory of Micro-nano Measurement Manipulation and Physics Ministry of Education Beihang University Beijing 100191 China

Zn0.95Co0.05O and Zn0.91Co0.05Cu0.04O thin films were fabricated on Si（111） substrate by the reactive magnetron sputtering method at different O-Ar *** characterizations by X-ray diffractometry（XRD）,X-ray photo-electronic spectrum（XPS）, and electron paramagnetic resonance（EPR） indicate that the doped Cu ions substitute the Zn2.ions in the ZnO *** doped Cu ions are in Cu+and Cu2.mixture valence *** ferromagnetism of the Zn0.91Co0.05Cu0.04O film increases gradually the increasing Cu+ions *** results indicate that the increase of ferromagnetism is not owing to the magnetic contribution of Cu+ions themselves,but owing to the enhancement of magnetic interaction between Co ions,which suggests that p-type doping of Cu+ions plays an important role in mediating the ferromagnetic coupling between Co ions.

关键词： Zn0.95Co0.05O films Zn0.91Co0.05Cu0.04O films magnetic semiconductors magnetic coupling co-doping

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Dynamical Evolution of Gamma-Ray Burst Remnants with Evolving Radiative Efficiency

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chinese Journal of Astronomy and Astrophysics 2002年第6期2卷 525-532页

作者： Jin-Bo Feng, Yong-Feng Huang, Zi-Gao Dai and Tan Lu1 Department of Astronomy, Nanjing University, Nanjing 2.00932.LCRHEA, institute for high-{2. physics, chinese academy of sciences, beijing 100039 LCRHEA Institute for High-Energy Physics Chinese Academy of Sciences Beijing 100039

In previous works, a generic dynamical model has been suggested by Huang. et al., which is shown to be correct for both adiabatic and radiative blast-waves, in both ultra-relativistic and non-relativistic phases. In deriving their equations, Huang et al. have assumed that the radiative efficiency of the fireball is constant. They then applied their model directly to realistic cases where the radiative efficiency evolves with time. In this paper, we abandon the above assumption and re-derive a more accurate dynamical equation for gamma-ray burst remnants. Numerical results show that the model presented by Huang et al. is accurate enough in general cases.

关键词： gamma rays : bursts hydrodynamics radiation mechanisms : nonthermal

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INVESTIGATION OF BONDING IN NANO-SiO_(2. BY Si L_(2.3) X-RAY ABSORPTION NEAR-EDGE STRUCTURE SPECTROSCOPY

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Acta Metallurgica Sinica(English Letters) 2001年第6期14卷 473-478页

作者： Z.Y. Wu, K. Ibrahim, G. Li, J. Zhang, F.Q. Liu, M.I Abbas, R. Hu, H.J. Qian and F.Q. Tang ( beijing synchrotron {2. Facility, institute of high energy physics, the chinese academy of sciences, beijing 100039, china) ( Laboratori Nazionali di Frascat Inst. of high energy Phys. Chinese Acad. of Sci. Beijing 100039 China

The Si L2.3 X-ray absorption near-edge structure (XANES) can be used to probe thelocal structure around Si and derive electronic information of the unoccupied s- andd-like partial density of states in nano-size SiO2. We present Si L2.3-edge for threedifferent size silicates acquired by total electron yield (TEY) at the photoemission sta-tion of beijing synchrotron {2. Facility (BSRF). The Si L2.3-edge spectra areinterpreted based on ab initio full multiple-scattering (MS) calculation. The Si L2.3-edge of nano-size materials has XANES similar to that of a-quartz. The similaritiesbetween the Si L2.3-edge shapes attest to a common molecular-orbital picture of theirSi-O bonding and the same coordination state. However, a considerable broadeningof Si L2.3-edge XANES spectra as decrease of particle size is also an indicative ofpolyhedral distortions.

关键词： multiple scattering calculation X-ray absorption nano particle electronic structure

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