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Journal of Huazhong University of Science and Technology(medical Sciences) 1995年第2期15卷 120-124页

作者：韩庆红舒沪英王建任恕 department of traditional {2. Medicine Tongji Hospital Wuhan

The pregnant rats with cigarette smoking exposure were used as the animal model for studying the pathogenesis of IUGR. The mechanism of preventing intrauterine growth retardation with 'Foshousan' plus Danshen by protecting erythrocytes against lipid peroxidation damage was also preliminarily *** erythrocyte membrane lipid peroxide content, erythrocyte SOD activities were detected by ultraviolet split-beam spectrophotometry and chemiluminescence technique. The results showed that the erythrocyte MDA levels and ratio of abnormal erythrocyte in model group were significantly higher than those in control group and treated group, and the fetal mean birth weight, erythrocyte SOD levels were all statistically significantly lower than those in control group and treated group. The fetal birth weight showed a significant positive correlation with SOD levels and a significant negative correlation with MDA level. The results suggest 'Foshousan' plus Danshen can protect erythrocytes against lipid peroxidation damage, thus conserving the normal form, structure and function of erythrocytes, improving the uteroplacental blood flow and thereby increasing the fetal mean birth weight.

关键词： intrauterine growth retardation (IUGR) erythrocyte lipid peroxidation Foshousan

Differentiation of Rabbit Bone Marrow Mesenchymal Stem Cells to Myogenic Cells and Expression of VEGF After Gene Transfection

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Differentiation of Rabbit Bone Marrow Mesenchymal Stem Cells...

Effects of salvianolic acid B on proliferation, neurite outgrowth and differentiation of neural stem cells derived from the cerebral cortex of embryonic mice

第8届中国南方国际心血管病学术会议

作者： Sheng Xiaogang~1 Chen Qiuxiong~1 Song Hui~2.Feng Jianzhang~3 1 department of Cardiology Guangdong Provincial hospital of {2. chinese Medicine Guangzhou 510100 2.department of Pharmacology Shantou University medical College Shantou 515031 3 Guangdong Provincial Cardiovascular Institute Guangzhou 510080

＜正＞Objectives To induce the differentiation of rabbit bone marrow mesenchymal stem cells（MSCs） to myogenic cells in vitro,and to investigate the expression of vascular endothelial growth factor（VEGF） gene in MSCs transfected by *** MSCs were isolated and purified from rabbit bone marrow by percoll（1.073g/ml） and then cultured in low glucose DMEM with 10%FBS. AdTrackCMV-VEGF165 eukaryotic expression vector was constructed and transfected into the *** being incubated with 5-azacytidine（5-Aza）,the expression of troponin I in MSCs was assayed by immunohistochemistry and the expression of VEGF gene was identified by northern blot and western *** concentration of VEGF in the supernatant was measured by *** MSCs were isolated and cultured successfully from rabbit bone marrow. The positive cTnI stain of some MSCs after the induction of 5-aza indicated that the cells were differentiated to myogenic *** blot and western blot showed that the expression of VEGF 165 mRNA was much higher in the hVEGF165 gene transfected cells than that of the control. The concentration of VEGF in the supernatant got to the peak 3-5 days after hVEGF165 gene transfection（1011-102.pg/ ml） and decreased gradually thereafter,but still higher than that of control group or pAdTrackCMV group（349pg/mL vs 116 pg/mL or 12.pg/ml respectively,P＜0.01）.Conclusion MSCs could be induced to differentiate to myogenic cells by 5-aza in vitro and could express VEGF by VEGF gene transfection.

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Science China(Life Sciences) 2010年第6期53卷 653-662页

作者： GUO GuoQing1,LI Bin1,WANG YuanYuan1,SHAN AiJing1,SHEN WeiZai1,YUAN Lin2.& ZHONG ShiZhen2.1department of Anatomy,medical College of Jinan University,Guangzhou 510630,China 2.nstitute of Clinical Anatomy,Key Laboratory of Tissue Construction and Detection of Guangdong Province,Southern medical University,Guangzhou 510150,China department of Anatomy Medical College of Jinan University

Salvianolic acid B is isolated from Salvia miltiorrhiza,the root of which is widely used as a traditional chinese medicine to treat ***,little is known about how salvianolic acid B influences growth characteristics of neural stem cells (NSCs).The purpose of the present study was to evaluate the effects of salvianolic acid B on proliferation,neurite outgrowth and differentiation of NSCs derived from the cerebral cortex of embryonic mice using MTT,flow cytometry,immunofluorescence and *** was found that 2. μg mL·1 and 40 μg mL·1 salvianolic acid B had similar effects on proliferation of NSCs,and a suitable concentration of salvianolic acid B increased the number of NSCs and their derivative *** growth-promoting activity of salvianolic acid B was dependent on and associated with an accumulation in the G2.S-phase cell *** acid B also promoted the neurite outgrowth of NSCs and their differentiation into *** mRNA for tau,GFAP and nestin were present in differentiating neurospheres induced by salvianolic acid ***,high-level expression of tau mRNA and low-level expression of GFAP mRNA was detected in differentiated cells,in contrast to the control *** collective evidence indicates that exogenous salvianolic acid B is capable of promoting proliferation of neurospheres and differentiation towards the neuronal lineage in vitro and may act in the proliferation of NSCs and may promote NSC differentiation into neuronal cells.

关键词： salvianolic acid B proliferation differentiation neurite outgrowth neural stem cells cerebral cortex embryonic mouse

Effects of Hesl signaling pathway on differentiation of bone marrow stromal cells into neurons in vitro

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Effects of Hesl signaling pathway on differentiation of bone...

HB(N5)3M(M=Li,Na,K,and Rb):A new kind of pentazolides as HEDMs

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Zhong-Li HU~1,Hong-En WEI~2.Chun-Fang WANG~(3,*) 1 hospital of traditional {2. Medicine of Taiyuan,Taiyuan,030009 2.department of Anatomy & Institute of Biomedical Engineering,Second Military medical University,Shanghai 2.0433 3 department of Biotechnology,Shanxi medical University,Taiyuan 030001

＜正＞At present our understanding of the molecular mechanisms underlying the bone marrow stromal cells（BMSCs） differentiating into neural cells is still *** signaling pathways,such as Hes1 signaling molecular,have an important role in neural stem cell *** this study,the putative involvement of the Hes1 signaling pathway in the neuronal differentiation of BMSCs was investigated through RNA interference（RNAi）.We isolated BMSCs from adult rats with complete bone marrow blood *** found that Hes1-siRNA could efficiently block expression of Hes1 in *** induction,immunocytochemistry,reverse transcriptase-PCR and Western blot analysis,all indicated that the expression of neuron-specific markers such as neuron-specific enolase was higher in Hes1-siRNA BMSCs than that in control *** the level of Mash1 expression increased in Hes1-siRNA BMSCs than that in control *** data indicate that Hes1-Mash1 signaling plays a role in the differentiation of BMSCs into neurons in *** or genetic interference with Hes1 signaling may provide a novel method to obtain neurons for therapeutic use.

关键词： bone marrow stromal cells differentiation neurons Hes1

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Progress in Natural Science:Materials International 2009年第1期19卷 41-45页

作者： Jian Cui a,Yan Zhang a,Fengjuan Zhao a,Jing Yang b,Gangyi Shen a,Yu Xu a,a chinese Minority traditional {2. Center,College of Live and Environmental Sciences,Central University for Nationalities,Beijing 100081,China b department of Chemistry,Tangshan Teacher’s College,Tangshan 063000,China chinese Minority traditional {2. Center College of Live and Environmental Sciences Central University for Nationalities Beijing 100081 China department of Chemistry Tangshan Teacher’s College Tangshan 063000 China

HB(N5)3M(M=Li,Na,K,and Rb) have been investigated as potential high-energy density materials in the present paper by means of density functional *** all show kinetic stabilities as to the breakup of N5 *** kinetic stabilities decrease in the order of Li,Na,K,and *** to HB(N5)3K and HB(N5)3Rb,HB(N5)3Li and HB(N5)3Na have higher transition state barriers and are of significantly higher energy content,and thus they are suggested to be more practical and suitable as potential high-energy density materials.

关键词： DFT Pentazolide HEDM

Effect of Xiaofenghuoxue decotion on Th1 and Th2.cytokine production in BALF in allergen-induced asthma guinea pigs

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Effect of Xiaofenghuoxue decotion on Th1 and Th2 cytokine pr...

CURATIVE EFFECT OBSERVATION OF LASER NEEDLE TREATMENT IN 76 CASES OF PAIN

两岸三地药理学与临床药理学术会议

作者： LI Hong~1 XIA Ming-yu~2.CHEN Li~1 ZHOU Lian-di~3 WANG Zhi-hui~2.HUANG Jianan~4 ZHANG Qi-hui~5 (1. department of Respiratory Medicine, Kunshan hospital of {2. chinese 2.5300 2. School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang 110016 China 3. department of Immunology, China medical University, Shenyang 110016 4. department of Respiratory Medicine, First Affiliated hospital of Soochow University 2.5006 5. School of {2. chinese Material Medica Shenyang Pharmaceutical University, Shenyang 110016 China)

Objective To investigate the effect of Xiaofenghuoxue decotion (XFHX) on the production of Th1 and Th2.cytokine in bronchoalveolar lavage fluid (BALF) in asthma guinea pigs sensitized and chal- lenged with *** The animals were randomly divided into 5 groups:the normal control group,the asthma group,the XFHX high dose group,the XFHX low dose group and the budesonide group. 2.weeks after sensitization with a mixture of 10 % OVA and 10 % Al(OH),the drugs were given to the an- imals for 7 days,and then the animals were challenged with aerosol of 1% ovalbumin,the latency time for the onset of respiratory abnormalities was examined,BALF was collected 16 h *** in BALF was *** level of IFN-γreduced by Th1 cells and IL-4 and IL-5 produced by Th2.cells were deter- mined by *** contents were also *** The latency time for the onset of respiratory ab- normalities and leukocyte infiltration in both XFHX high dose group and low dose group significantly de- creased compared with the asthma *** level of IFN-γin both XFHX high dose group and low dose group significantly increased compared with the asthma group,the level of IL-4 and IL-5 in both XFHX high dose group and low dose group significantly decreased compared with the asthma *** produc- tion of NO was inhibited in both XFHX high dose group and low dose group compared with the asthma *** These results suggest that the anti-asthma effect of Xiaofenghuoxue decotion is associat- ed with NO regulated reversal of Th1/Th2.cytokine balance in allergen-induced asthma guinea pigs.

关键词： Xiaofenghuoxue asthma IFN-γ IL-4 IL-5 NO

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针刺研究 1989年第Z1期 260-260页

作者： Hu Guangzhi department of traditional {2. Medicine, The First Teaching hospital of Chongqing University of medical Sciences

'Laser Needle', low output laser irradiation analgesia is effective in thetherapy of pain. The author used 3mW He-Ne laser irradiation directly on theacupuncture point or on the painful area in a study of herpes zoster, trigeminalneuralgia, aphthous ulcer, and others with 93.4% effective and 61.8% *** needle has the advantage of no pain, no possibility of infection,and especially adequate to aged and children.

关键词： CURATIVE EFFECT OBSERVATION OF LASER NEEDLE TREATMENT IN 76 CASES OF PAIN

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维医沙疗传热效应抗炎作用的实验研究

中南大学学报(医学版) 2009年第1期34卷 1-7页

作者：卫荣迪丽娜尔·马合木提哈木拉提·吾甫尔新疆医科大学一附院中医科新疆大学机械工程学院力学教研室新疆医科大学院办

目的:探讨维吾尔医沙疗过程中传热效应对兔膝骨关节炎实验性动物的抗炎作用。方法:建立室内沙疗实验平台,采用2.00型多通道数据采集仪,采集实验动物沙疗过程中皮肤表面温度和沙体各层的温度随时间变化的数据。于新西兰兔右膝关节腔内注... 详细信息

目的:探讨维吾尔医沙疗过程中传热效应对兔膝骨关节炎实验性动物的抗炎作用。方法:建立室内沙疗实验平台,采用2.00型多通道数据采集仪,采集实验动物沙疗过程中皮肤表面温度和沙体各层的温度随时间变化的数据。于新西兰兔右膝关节腔内注射木瓜蛋白酶,再随机分成沙疗组、芬必得组及空白对照组。从造模第15天开始,沙疗组予埋沙治疗,芬必得组予芬必得灌胃,空白对照组不作任何处理。第30天留取血清及关节液标本以备检测白介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）及一氧化氮（NO）的水平。取滑膜及软骨组织作组织病理学观察。结果:皮肤温度在沙疗开始阶段上升,随后逐渐降低;沙疗组的关节软骨的病变程度较其他两组均有显著减轻,沙疗组及芬必得组关节液中的IL-1β和TNF-α的水平及血清中NO水平较空白对照组明显降低。结论:维吾尔医沙疗能够抑制骨关节炎的关节软骨的炎症,减轻软骨破坏。其机制可能通过沙疗过程中传热效应抑制关节液中细胞因子水平的异常升高而发挥作用。

关键词：维医沙疗传热效应抗炎作用

2.例牙周病合并错畸形综合治疗的临床体会

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泰州职业技术学院学报 2011年第3期11卷 84-85页

作者：谷芳泰州市中医院口腔科江苏泰州225300

目的探索牙周病合并错畸形的治疗方法。方法通过对2.例牙周病合并错畸形采用彻底牙周治疗的基础上联合正畸治疗,并在治疗过程中和治疗结束后一年内定期进行牙周检查。结果所有2.例牙周病合并错畸形病例通过综合治疗不仅错畸形... 详细信息

目的探索牙周病合并错畸形的治疗方法。方法通过对2.例牙周病合并错畸形采用彻底牙周治疗的基础上联合正畸治疗,并在治疗过程中和治疗结束后一年内定期进行牙周检查。结果所有2.例牙周病合并错畸形病例通过综合治疗不仅错畸形得以矫治,咬关系得以调整,而且有效控制了牙周炎症,消除了创伤,有利于牙周组织恢复健康,达到了美容和恢复咀嚼功能的目的。结论综合法治疗牙周病合并错畸形,方法简单,效果良好,是一种易于推广,行之有效的治疗手段之一。

关键词：牙周病错畸形牙周治疗正畸治疗

Analysis on the Effect of Acupuncture in Treating Cervical Spondylosis with Different Syndrome Types

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chinese Journal of Integrative Medicine 2009年第6期15卷 426-430页

作者：符文彬梁兆晖朱晓平于鹏张继福 Acupuncture and Moxibustion department of Guangdong Provincial hospital of {2. chinese medical Sciences Guangdong Provincial Academy of chinese Medicinal Sciences

Objective:To observe the clinical effect of acupuncture in treating cervical spondylosis with different syndrome ***:One hundred and seventeen patients were randomized into the treated group (59 cases),treated with normal acupuncture,and the control group(58 cases),treated with sham acupuncture, operated once every other day,9 times in total(in 18 days) as one therapeutic course,and a succeeding 3-month follow-up study was carried out after terminating the *** efficacy of treatment was eva...

关键词： acupuncture cervical spondylosis efficacy evaluation repeated measurement