Differentiation of Rabbit Bone Marrow Mesenchymal Stem Cells to Myogenic Cells and Expression of VEGF After Gene Transfection
会议名称：《第8届中国南方国际心血管病学术会议》
会议日期：2006年
学科分类：1001[医学-基础医学(可授医学、理学学位)] 100101[医学-人体解剖与组织胚胎学] 10[医学]
摘 要：正Objectives To induce the differentiation of rabbit bone marrow mesenchymal stem cells（MSCs） to myogenic cells in vitro,and to investigate the expression of vascular endothelial growth factor（VEGF） gene in MSCs transfected by *** MSCs were isolated and purified from rabbit bone marrow by percoll（1.073g/ml） and then cultured in low glucose DMEM with 10%FBS. AdTrackCMV-VEGF165 eukaryotic expression vector was constructed and transfected into the *** being incubated with 5-azacytidine（5-Aza）,the expression of troponin I in MSCs was assayed by immunohistochemistry and the expression of VEGF gene was identified by northern blot and western *** concentration of VEGF in the supernatant was measured by *** MSCs were isolated and cultured successfully from rabbit bone marrow. The positive cTnI stain of some MSCs after the induction of 5-aza indicated that the cells were differentiated to myogenic *** blot and western blot showed that the expression of VEGF 165 mRNA was much higher in the hVEGF165 gene transfected cells than that of the control. The concentration of VEGF in the supernatant got to the peak 3-5 days after hVEGF165 gene transfection（1011-1027pg/ ml） and decreased gradually thereafter,but still higher than that of control group or pAdTrackCMV group（349pg/mL vs 116 pg/mL or 125pg/ml respectively,P0.01）.Conclusion MSCs could be induced to differentiate to myogenic cells by 5-aza in vitro and could express VEGF by VEGF gene transfection.