Effects of Hesl signaling pathway on differentiation of bone marrow stromal cells into neurons in vitro

作     者：Zhong-Li HU~1,Hong-En WEI~2,Chun-Fang WANG~(3,*) 1 Hospital of Traditional Chinese Medicine of Taiyuan,Taiyuan,030009 2 Department of Anatomy & Institute of Biomedical Engineering,Second Military Medical University,Shanghai 200433 3 Department of Biotechnology,Shanxi Medical University,Taiyuan 030001 

会议名称：《中国神经科学学会第四次会员代表大会暨第八届全国学术会议》

会议日期：2009年

学科分类：0710[理学-生物学] 07[理学] 071006[理学-神经生物学] 

基　　金：supported by National Natural Science Foundation of China(30672114) Natural Science Foundation of Shanxi Province(2009011057-1) 

关 键 词：bone marrow stromal cells differentiation neurons Hes1 

摘      要：正At present our understanding of the molecular mechanisms underlying the bone marrow stromal cells（BMSCs） differentiating into neural cells is still *** signaling pathways,such as Hes1 signaling molecular,have an important role in neural stem cell *** this study,the putative involvement of the Hes1 signaling pathway in the neuronal differentiation of BMSCs was investigated through RNA interference（RNAi）.We isolated BMSCs from adult rats with complete bone marrow blood *** found that Hes1-siRNA could efficiently block expression of Hes1 in *** induction,immunocytochemistry,reverse transcriptase-PCR and Western blot analysis,all indicated that the expression of neuron-specific markers such as neuron-specific enolase was higher in Hes1-siRNA BMSCs than that in control *** the level of Mash1 expression increased in Hes1-siRNA BMSCs than that in control *** data indicate that Hes1-Mash1 signaling plays a role in the differentiation of BMSCs into neurons in *** or genetic interference with Hes1 signaling may provide a novel method to obtain neurons for therapeutic use.