检索结果-南通市图书馆

Inhibitory Effect of CT120B,an Alternative Splice Variant of CT120A,on Lung cancer Cell Growth

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2005年第9期37卷 588-592页

作者： Dong-Ning PAN~(1,2) Jin-Jun LI~(2,3) Lin WEI~2 Ming YAO~(2,3) Da-Fang WAN~(2,3*) Jian-Ren GU~(1,2,3*) 1 shanghai Medical College,Fudan University,shanghai 200032,china 2 national laboratory for oncogenes and related genes,shanghai cancer institute,shanghai 200032,china 3 Medical School of shanghai Jiaotong University,shanghai 200032,china shanghai Medical College Fudan UniversityShanghai 200032 China national laboratory for oncogenes and related genes Shanghai Cancer InstituteShanghai 200032 China Medical School of shanghai Jiaotong Universityshanghai 200032 China

The expression product of ct120a,a novel gene isolated from human chromosome 17p13.3in our laboratory,was predicted to have seven transmembrane domains and could cause malignanttransformation of mouse NIH3T3 *** existed an mRNA splicing variant of ct120a,namely ct120b,which had a 96-nucleotide deletion and produced an in-frame loss of 32 amino acids from codon 136 tocodon 167 of *** CT120B protein was predicted to have six transmembrane *** thisstudy,we observed that the green fluorescent protein-tagged CT120B was localized on plasma membraneand in cytoplasm in SPC-A-1 *** expression of CT120B/A in normal lung tissue and in lung cancercells was also *** showed that the stable CT120B overexpression in SPC-A-1 cells resultedin a reduction of cell growth rate,and inhibited tumorigenecity and anchorage-independent growth in *** functions of CT120A and CT120B for cell growth appeared *** suggested that thedelayed G1/S phase transition might contribute to the inhibitory activities of CT120B on cell growth and thatthe deleted 32 amino acids missing in CT120B might be essential for the oncogenetic activities of CT120A.

关键词： CT120B alternative splicing cell growth lung cancer

The tumor-selective over-expression of the human Hsp 70 gene is attributed to the aberrant controls at both initiation and elongation levels of transcription

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Cell Research 2003年第2期13卷 93-109页

作者： LING CAI, JING DE ZHU,The State-key laboratory for oncogenes and related genes, shanghai cancer institute, LN 25/2200, Xie-tu Road, shanghai 200032, china cancer Genetics and Gene Therapy The State-key Laboratory for Oncogenes and Related Genes Shanghai Cancer Institute Shanghai Jiao Tong University LN 25/2200 Shanghai China

The tumor selective over-expression of the human Hsp70 gene has been well documented in human tumors, linked to the poor prognosis, being refractory to chemo- and radio-therapies as well as the advanced stage of tumorous lesions in particular. However, both the nature and details of aberrations in the control of the Hsp70 expression in tumor remain enigmatic. By comparing various upstream segments of the Hsp70 gene for each’s ability to drive the luciferase reporter genes in the context of the tumor cell lines varying in their p53 status and an immortal normal liver cell line, we demonstrated in a great detail the defects in the control mechanisms at the both initiation and elongation levels of transcription being instrumental to the tumor selective profile of its expression. Our data should not only offer new insights into our understanding of the tumor specific over-expression of the human Hsp70 gene, but also paved the way for the rational utilization of the tumor selective mechanism with the Hsp70 a

关键词： Hsp 70 Tumor transcription elongation 5’ UTR. Over-expression.

The altered DNA methylation pattern and its implications in liver cancer

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Cell Research 2005年第4期15卷 272-280页

作者： Jing De ZHU1,2,*1The State-key laboratory for oncogenes and related genes, shanghai cancer institute, shanghai Jiaotong University, LN 2200/25, Xietu Road, shanghai 200032, china.2The Collage of Life Sciences, Zhejiang Science and Technology University, Xiasha University District, Hangzhou, Zhejiang 310018, china. The State-key laboratory for oncogenes and related genes Shanghai Cancer Institute Shanghai Jiaotong University LN 2200/25 Shanghai China The Collage of Life Sciences Zhejiang Science and Technology University Xiasha University District Hangzhou Zhejiang China

DNA methylation is the most intensively studied epigenetic phenomenon, disturbances of which result in changes ingene transcription, thus exerting drastic imparts onto biological behaviors of cancer. Both the global demethylation andthe local hypermethylation have been widely reported in all types of tumors, providing both challenges and opportunitiesfor a better understanding and eventually controlling of the malignance. However, we are still in the very early stage ofinformation accumulation concerning the tumor associated changes in DNA methylation pattern. A number of excellentrecent reviews have covered this issue in depth. Therefore, this review will summarize our recent data on DNA methy-lation profiling in cancers. Perspectives for the future direction in this dynamic and exciting field will also be given.

关键词： DNA methylation epigenetics liver cancer tumor staging and classification.

The inhibitory effect of transthyretin gene on growth of human hepatoma cells

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Cell Research 1994年第1期4卷 89-96页

作者： LIU CHAOTING JIN YAO DAIZONG LI HUIQIU JIANG JIANREN GU.(national laboratory for oncogenes and related genes,shanghai cancer institute, shanghai 200032, china.) national laboratory for oncogenes and related genes Shanghai Cancer Institute Shanghai China

Transthyretin(TTR) gene was highly expressed in normal liver and it has been found to be deleted in part of DNA samples from human hepatic cancer. Its mRNA expression was suppressed in most hepatoma samples. In order to study the biological effect of TTR gene on the growth of hepatoma cells, a recombinant vector containing TTR cDNA was constructed by pCMV, then it was transfected into hepatoma cell lines SMMC-7721 and *** has been demonstrated that the inhibition of growth rate of TTR cDNA transfected hepatoma cells was about 50%in strength compared with that of the control. This inhibition was further enhanced when the transfected hepatoma cells were treated with all-trans retinoic acid. Hepatoma cells of cell lines PLC/PRF/5, SMMC-7721 and Q3 as well as hepatoma cells SMMC-7721 transfected with pCMV or pCMV-TTR were analyzed for TTR expression by Northern hybridization. The low level of TTR expression was found in both hepatoma cell lines and in SMMC-7721cells transfected with pCMV alone. However, a remarkable TTR mRNA expression was observed in hepatoma SMMC-7721 cells transfected with pCMV-TTR. It seems possible that TTR gene might be a candidate of cancer suppressor gene for human hepatic cancer.

关键词： TTR cDNA hepatoma growth RNA retinoic acid

Methylation profiling of twenty four genes and the concordant methylation behaviours of nineteen genes that may contribute to hepatocellular carcinogenesis

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Cell Research 2003年第5期13卷 319-333页

作者： JIAN YU, HONG Yu ZHANG ZHEN ZHONG MA, WEI LU, YI FEI WANG, JINGDE ZHU The State-Key laboratory for oncogenes and related genes, shanghai cancer institute, shanghai Jiaotong University, LN 2200/25, Xietu Road, shanghai 200032, china E-mail: zhujingde@*** 2Department of Mathematics, shanghai University, No. 99, Shangda Road, shanghai 200436, china The State-Key laboratory for oncogenes and related genes Shanghai Cancer Institute Shanghai Jiaotong University Shanghai China Department of Mathematics Shanghai University Shanghai China

To determine the possible role of the epigenetic mechanisms in carcinogenesis of the hepatocellular carcinoma, we methylation-profiled the promoter CpG islands of twenty four genes both in HCC tumors and the neighboring non-cancerous tissues of twenty eight patients using the methylation-specific PCR （MSP） method in conjunction with the DNA sequencing. In comparison with the normal liver tissues from the healthy donors, it was found that while remained unmethylated the ABL, CAV, EPO, GATA3, LKB1, NEP, NFL, NIS and p27KIP1 genes, varying extents of the HCC specific hypermethylation were found associated with the ABO, AR, CSPG2, cyclin al, DBCCR1, GALR2, IRF7, MGMT, MT1A, MYOD1, OCT6, p57KIP2, p73, WT1 genes, and demethylation with the MAGEA1 gene, respectively. Judged by whether the hypermethylated occurred in HCC more frequently than in their neighboring normal tissues, the hypermethylation status of the AR, DBCCR1, IRF7, OCT6, and p73 genes was considered as the event specific to the late stage,

关键词： promoter CpG island methylation specific PCR concordant behaviors of methylation.

A novel gene delivery system targeting cells expressing VEGF receptors

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Cell Research 1999年第1期9卷 11-25页

作者： LI JUN MIN JUN SONG HAN YI HUANG PEI KUN TIAN SHU MIN QU MIN YAO HUI QIU JIANG DA FANG WAN JING CHU LUO CHENG XIAO GU JIAN REN GU( national Labomtory for oncogenes and related genes, shanghai cancer institute, shanghai 200032,china)(national laboratory of national laboratory for oncogenes and related genes Shanghai Cancer Institute Shanghai China Zhejiang Taizhou Normal College Zhejiang China national laboratory of Protein Engineering and Plant Genetic Engineering Peking University Beijing China

Two ligand oligopeptides GV1 and GV2 were designed according to the putative binding region of VEGF to its receptors. GV1, GV2 and endosome releasing oligopeptide HA20 were conjugated with poly-L-lysine or protamine and the resulting conjugates could interact with DNA in a noncovalent bond to form a complex. Using pSV2-β-galactosidase as a reporter gene, it has been demonstrated that exogenous gene was transferred into bovine aortic arch-derived endothelial cells (ABAE) andhuman malignant melanoma cell lines (A375) in vitro. In vivo experiments, exogenous gene was transferred into tumor vascular endothelial cells and tumor cells of subcutaneously transplanted human colon cancer LOVO, human malignant melanoma A375 and human hepatoma graft in nude mice. This system could also target gene to intrahepatically transplanted human hepatoma injected via portal vein in nude mice. These results are correlated with theGene delivery system targeting VEGF receptors relevant receptors (flt-1, flk-1/KDR) expression on the targeted cells and tissues.

关键词： VEGF receptors gene delivery system tumor vascular endothelial cells targeting

Application of EGFP-EGF fusions to explore mechanism of endocytosis of epidermal growth factor

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2007年第1期28卷 111-117页

作者： Hua JIANG~2 Jie ZHANG~(2,3) Bi-zhi SHI~(2,3) Yu-hong XU~3 Zong-hai LI~(2,3,4) Jian-ren GU~(2,3,4)2 State Key laboratory of oncogenes and related genes,shanghai cancer institute,Medical College of Fudan University 3 School of Pharmacy,shanghai Jiaotong University,shanghai 200032,china State Key laboratory of oncogenes and related genes Shanghai Cancer Institute Medical College of Fudan University Shanghai China School of Pharmacy Shanghai Jiaotong University Shanghai China

Aim:To develop a simple method for monitoring protein localization of epidermalgrowth factor(EGF)in living ***:Enhanced green fluorescent protein(EGFP)was used as an autofluorescent tag to label EGF ***-PAGE andWestern blot analysis were used to detect the expression of the EGFP-tagged EGF(EGFP-EGF)*** cell-binding and internalization activity of EGFP-EGFwere analyzed by fluorescence-activated cell sorting(FACS)and confocal ***:EGFP-EGF protein was expressed in Escherichia coli and purified.A cell-binding assay demonstrated that the EGFP-EGF protein could bind effi-ciently to the cells expressing *** binding and internalization of EGFP-EGFcan be visualized even at a very low concentration under confocal *** FACS-based assay for internalization activity indicated the accumulation ofinternalized EGFP-EGF over ***,the results of the competitionassay indicated its EGFR binding *** such a method,it does notneed to label EGF with chemicals and avoid light in the experimental ***:The fusion protein EGFP-EGF has several characters including highsensitivity,stability and convenience for manipulation,and is a powerful tool forthe study of EGF endocytosis.

关键词： receptor epidermal growth factor endocytosis purification expression

A novel protein-DNA interaction involved with the CpG dinucleotide at -30 upstream is linked to the DNA methylation mediated transcription silencing of the MAGE-A1 gene

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Cell Research 2004年第4期14卷 283-294页

作者： Jie ZHANG, Jian YU, Jun GU, Bao Mei GAO, Ying Jun ZHAO, Peng WANG, Hong Yu ZHANG, Jing De ZHU The State-key laboratory for oncogenes and related genes, shanghai cancer institute, shanghai Jiao Tong University, LN 2200/25, Xietu Road, shanghai 200032, china. The State-key laboratory for oncogenes and related genes Shanghai Cancer Institute Shanghai Jiao Tong University LN 2200/25 Shanghai China

To understand the DNA-methylation mediated gene silencing mechanisms, we analyzed in cell culture of the promoter function of the MAGE-A1 gene, which is frequently demethylated and over-expressed in human hepatocellular carcinoma. We have established the correlation of the DNA methylation of the promoter CpG island with expression status of this gene in a panel of the established liver cancer cell lines. The crucial CpG dinucleotide（s） within the minimal promoter subjected to the control mediated by DNA methylation with profound biological functions was also delineated. Furthermore, a novel sequence-specific DNA-protein interaction at the -30 CpG dinucleotide upstream of the gene was found having a vital part to play in the DNA methylation mediated transcription silencing of the MAGE-A1 gene. Our results would not only provide new insights into the DNA methylation mediated mechanisms over transcription of the MAGE-A1 gene, but also pave the way for further defining the cross-talk among DNA methylation, h

关键词： MAGE-A1 DNA methylation transcriptional regulation.

Differential expression of a cDNA clone in human liverversus hepatic cancer-highly homologous to aryl-dialkyl-phosphatase

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Cell Research 1997年第1期7卷 79-90页

作者： WANG KAN KAN DA FANG WAN XIAO KUN QIU PEI XIN LU JIAN REN GU(national La6oratory for oncogenes and related genes shanghai cancer institute, shanghai 200032)(Qidong cancer institute, Jiangsu 226200) national laboratory for Oneogenes and related genes shanghai cancer institute Shanghai Qidong cancer institute diangsu

We applied the technique of mRNA differential display to normal liver tissue and hepatoma cell line Hep3B. One of the isolated cDNA clones was expressed in human normal liver tissue but not in the human hepatocarcinoma cell line. Northern Blot analysis confirmed that high level of mRNA was expressed in human normal liver tissue but the level was decreased in non-cancerous liver tissue from hepatoma patients. Low level or no expression was observed in human hepatoma tissue. One of these transcripts was about 1.8 kb in length. Southern Blot analysis showed that it was a single copy gene. We obtained a full length cDNA clone of 2,395 bp by screening human liver 5'-stretch plus cDNA library. Nucleotide sequence indicated that this clone was highly homologous to aryldialkyl-phosphatase and possessed two polymorphic sites. Aryl-dialkyl--phosphatase which has a prominent role in the metabolism of several toxic, synthetic compounds, may be potentially related to human hepatocarcinoma susceptibility. The biological significance of its differential expression in normal versus malignant tissue is discussed.

关键词： Hepatocarcinoma mRNA DD aryl-dialkyl-phosphatase