The inhibitory effect of transthyretin gene on growth of human hepatoma cells
The inhibitory effect of transthyretin gene on growth of human hepatoma cells作者机构：National Laboratory for Oncogenes and Related Genes Shanghai Cancer Institute Shanghai China
出 版 物：《Cell Research》 (细胞研究(英文版))
年 卷 期：1994年第4卷第1期
页 面：89-96页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种]
主 题：TTR cDNA hepatoma growth RNA retinoic acid
摘 要：Transthyretin(TTR) gene was highly expressed in normal liver and it has been found to be deleted in part of DNA samples from human hepatic cancer. Its mRNA expression was suppressed in most hepatoma samples. In order to study the biological effect of TTR gene on the growth of hepatoma cells, a recombinant vector containing TTR cDNA was constructed by pCMV, then it was transfected into hepatoma cell lines SMMC-7721 and *** has been demonstrated that the inhibition of growth rate of TTR cDNA transfected hepatoma cells was about 50%in strength compared with that of the control. This inhibition was further enhanced when the transfected hepatoma cells were treated with all-trans retinoic acid. Hepatoma cells of cell lines PLC/PRF/5, SMMC-7721 and Q3 as well as hepatoma cells SMMC-7721 transfected with pCMV or pCMV-TTR were analyzed for TTR expression by Northern hybridization. The low level of TTR expression was found in both hepatoma cell lines and in SMMC-7721cells transfected with pCMV alone. However, a remarkable TTR mRNA expression was observed in hepatoma SMMC-7721 cells transfected with pCMV-TTR. It seems possible that TTR gene might be a candidate of cancer suppressor gene for human hepatic cancer.
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