检索结果-南通市图书馆

Cytoprotective effects of quercetin and its sugar-containing natural congeners in cultured HEK293 cells injured by anoxia/hypoglycemia and the struc-ture-effect relationship thereto

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Cytoprotective effects of quercetin and its sugar-containing...

The total flavonoids extracted from Xiaobuxin Tang reverse the hyperactivity of hypothalamic-pituitary-adrenal axis in chronically stressed rats

两岸三地药理学与临床药理学术会议

作者： JIN Yue~1 LV Yong~1 HAN Guo-zhu~1 YU Hong-shan~2 JIN Feng-xie~2 (1.Department of Clinical pharmacology,dalian medical university,dalian 116044 2. Department of Foodstuffs and Bio-engineering,dalian Polytechnology university, dalian 116034)

Objective To comparatively study anti-free radical and cytoprotective effects of quercetin(Q)and its monoglucoside isoquercetin(I),diglucoside rutin(R),which differs only in glycosyl-substitution at C-3 position of the molecules,using anoxia/hypoglycemia-induced cell injury model and thereby to explore the structure-effect relationship *** The cell injury model was established by HEK293 cells cul- tured in vitro with NaSO plus sugar-free Earle’s fluid as incubation medium;Cell survival rate(CSR), total antioxidant capacity(TAC),SOD and LDH levels were determined;The effect intensity of the 3 flavonoids were compared by means of IC,the concentration required to achieve 50% inhibition of the changes in the above indices in injured *** Q,I and R all concentration-dependently elevated CSR, TAC and SOD,and reduced LDH level;the all of IC50s for the above indices were ranked in order of ICI>*** The 3 structurally similar flavoloids all have significant and concentration-dependent anti-free radical and cyto-protective effects with the intensity being in order of aglycone>monoglucoside>diglucoside;the substitution of-OH by sugar group at C-3 position of flavoloids and increase in the sugar-substituent number are associated with the ef- fect intensity reduced;namely,the intensity of these effects of flavonoids is negatively related the substutu- tion by sugar group at C-3 position.

关键词： quercetin isoquercetin rutin flavonoid free-radical cytoprotection cell culture

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

沈阳药科大学学报 2008年第S1期25卷 104-104页

作者： AN Lei1,ZHANG You-zhi2(1.Department of pharmacology,dalian medical university,dalian 116044,china 2.Beijing Institute of pharmacology and Toxicology,Beijing 100850,china)

Objective To investigate the effect of XBXT-2 on the activity of hypothalamic-pituitary-adrenal(HPA)axis in chronic mild stress(CMS)model of *** Using ELISA to test the serum corticosterone,adrenocorticotropic hormone(ACTH)and corticotropin-releasing hormone(CRH)level in CMS rats;Using western blot to determine hippocampal glucocorticoids receptors(GR)expression in CMS *** Co-administration of XBXT-2(25,50 mg·kg-1,p.o.,28 days,the effective doses for behavioral responses)significantly decreased the serum corticosterone and ACTH level in CMS rats,while the CRH level was not markedly affected by chronic stress or ***,XBXT-2 significantly increased the GR expression in the hippocampus of CMS *** same effects were observed in the positive control drug imipramine(10 mg·kg-1,p.o.).Conclusions The decrease of serum corticosterone and ACTH level,as well as the increase of hippocampal GR expression may be the mechanisms underlying the antidepressant action of XBXT-2,which may associate with HPA axis.

关键词： Xiaobuxin-Tang hypothalamic-pituitary-adrenal axis glucocorticoid receptors stress

维普期刊数据库

Strong law of large numbers for Markov chains indexed by an infinite tree with uniformly bounded degree

在线全文

学校读者我要写书评

暂无评论

Science china Mathematics 2008年第2期51卷 195-202页

作者： HUANG HuiLin~1 YANG WeiGuo~(2+) 1 Department of Mathematics,Shanghai Jiaotong university,Shanghai 200240,china 2 faculty of Science,Jiangsu university,Zhenjiang 212013,china 1. Department of Mathematics Shanghai Jiaotong University Shanghai 200240 China 2. faculty of Science Jiangsu University Zhenjiang 212013 China

In this paper,we study the strong law of large numbers and Shannon-McMillan (S-M) theorem for Markov chains indexed by an infinite tree with uniformly bounded *** results generalize the analogous results on a homogene... 详细信息

关键词： Markov chains Shannon-McMillan theorem strong law of large numbers uniformly bounded tree 60F15 60J10

维普期刊数据库

Construction and Activity Assay of the Activating Transcription Factor 3 Reporter Vector pATF/CRE-luc

在线全文

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2006年第1期38卷 58-62页

作者： Jun-Qing XU~(1,2),Jing-Lan DENG~1,You-Sheng WU~2,Han-Yan FU~2,Rui-Hua WANG~2,Jian ZHANG~2, Fan LU~(2*),and Zhong-Liang ZHAO~(2*) ~1 Department of Naclear Medicine,Xijing Hospital,Fourth Military medical university,Xi'an 710032,china ~2 Department of Biochemistry and Molecular Biology,Fourth Military medical university,Xi'an 710032,china Department of Biochemistry and Molecular Biology Fourth Military Medical University Xi''an 710032 China

Activating transcription factor 3 (ATF3),a member of the activating transcription factor/cAMPresponsive element binding protein (ATF/CREB) family of transcription factors,is induced by manyphysiological *** investigate the activity of ATF/CREB in cells with physiological stresses,we developeda practical reporter vector,the plasmid pATF/CRE-luc,bearing activating transcription factor/cAMP responsiveelement (ATF/CRE) binding *** plasmid was constructed by inserting three repeats of the ATF/CREbinding element into the plasmid pG51uc,replacing the GAL-4 binding *** plasmids pACT/ATF3 andpATF/CRE-luc were transfected into HeLa and NIH3T3 cells,respectively,and the results showed that theexpression of luciferase .was increased in a dose-dependent manner on plasmid pACT/*** datasuggested that the plasmid pATF/CRE-luc could be used as a sensitive and convenient reporter system ofATF3 activity.

关键词： ATF/CRE luciferase gene pG51uc reporter system

Effect of tea polyphenols on cytochrome P450 and b5 content and mRNA expression of CYP2E1 and CYP1A2 in liver of mice

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Effect of tea polyphenols on cytochrome P450 and b5 content ...

Molecular mechanism about lymphogenous metastasis of hepatocarcinoma cells in mice

第三届中日双边药理学和临床药理学学术会议

作者： CHEN Xia HAN Guo-Zhu SUN Hui-Jun (Department of pharmacology,dalian medical university,dalian 116027,china)

AIM To investigate content of tea ployphe- nols(TPs) on cytochrome P450 and b5,and the expres- sion of CYP2E1 and CYP1A2 in livers of male mice re- ceiving *** ①Determination of the con- tent of TPs on eytochrome P450 and b5:TPs were injected intra-peritoneally to male mice at the doses of 25,50 and 100 mg·kg·d,respectively,for 2 *** positive control group was given chloramphenieol at a single dose of 50 mg·kg 1 h before sacrificing the *** negative control group was given the equal volume of saline solu- *** the contents of cytochrome P450 and b5 were determined by UV-spectrophotometry.②Determination of the expression of CYP2E1 and CYP1 A2:TPs were admin- istered intragastrically to male mice at the doses of 100, 200 and 400 mg·kg·d separatively for 5 *** model group and the negative control group were given the equal volume of saline *** the mice were given parac- etamol at the doses of 500 mg·kg at 1 h after TPs were administered last time except the mice in negative control ***2E1 and CYP1A2 mRNA expression levels in the liver were evaluated by *** The contents of cytochrome P450 and b5 in the livers of mice in three groups of TPs were dose-dependently decreased compared with the negative control *** CYP2E1 and CYP1 A2 mRNA expression levels of the TPs groups were dose-dependently decreased relative to the control group and the model *** TPs can suppress both cytochrome P450 and b5 in a dose depend- ent *** suppress cytochrome P450 isoform CYP2E1 and CYP1A2 mRNA *** results demonstrated that TPs defense paracetamol-induced liver damage by inhibiting mRNA expression of CYP2E1 and CYP1A2.

关键词： tea ployphenols CYP450 b5 CYP2E1 CYP1 A2 paracetamol

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2001年第4期7卷 532-536页

作者： Li Hou Ying Li Yong-Hua Jia Bo Wang Yi Xin Mao-Ying Ling Shen Lü Department of Pathology,dalian medical university,dalian 116027,Liaoning Province,chinaDepartment of Biochemistry,dalian medical university,dalian 116027,Liaoning Province,china Department of Pathology Dalian Medical University Dalian 116027 China. houli72@***

AIM: To investigate the correlation between lymphogenous metastasis and matrix metalloproteinases (MMPs) activity and the expression of Fas ligand of tumor cells in lymph nodes. METHODS: Fifty-six inbred 615-mice were equally divided into 2 groups and inoculated with Hca-F and Hca-P cells. Their lymph node metastatic rates were examined. Growth fraction of lymphocytes in host lymph nodes was detected by flow cytometry. The Hca-F and Hca-P cells were cultured with extract of lymph node, liver or spleen. The quantity of MMPs in these supernatants was examined by zymographic analysis. The expression of Fas ligand, PCNA, Bcl-2 protein of Hca-F and Hca-P cells in the mice were examined by immunohistochemistry. The apoptosis signals of macro-phages in lymph nodes were observed with in situ DNA fragmentation. RESULTS: On the 28th day post-inoculation, the lymph node metastatic rate of HcaF was 80%(16/20), whereas that of Hca-P was 25%(5/20). The growth fraction of lymphocytes was as follows: in the Hca-F cells, the proliferating peak of lymphocytes appeared on the 14th day post inoculation and then decreased rapidly, while in HcaP cells, the peak appeared on the 7th day post inoculation and then kept at a high level. With the extract of lymph node, the quantity of the MMP-9 activity increased (P2 were produced by both Hca-F and Hca-P tumor cells, which did not produce MMPs without the extract of lymph node or with the extracts of the liver and spleen. The expression of Fas Ligand of Hca-F cells was stronger than that of Hca-P cells (P 2 protein of Hca-F cells in the tumors of inoculated area were the same as that of Hca-P cells. In situ DNA fragmentation showed that the positive signals of macrophages were around Hca-F cells. CONCLUSION: Secretion of MMPs which was associated with metastatic ability of Hca-F and Hca-P tumor cells depends on the environment of lymph nodes. The increased expression

关键词： Animals DNA Fragmentation Flow Cytometry Gelatinase A Gelatinase B Liver Neoplasms, Experimental Lymph Nodes Lymphatic Metastasis Macrophages Membrane Glycoproteins Mice Mice, Inbred Strains Research Support, Non-U.S. Gov't Tumor Cells, Cultured

Research on Strategy of Prior Development of Physical Education

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Research on Strategy of Prior Development of Physical Educat...

Excretion of Four Catechins in Tea Polyphenols in Rats

2010 International Conference on Sport Medicine, Sport Science, and Computer Science (SMSSCS 2010)

作者： Ying Song 1 Bing Ren 2 1) dalian Neusoft Institute of Information china 116023 2) Teenagers Physical Health Research and Promotion Center dalian medical university china 116044

The prior development of Physical Education（P.E.） is the basis and fundamental requirement of a Sport Power *** refers to by putting the teenagers *** a prior position,pool the useful resources of school,family and society with a commission to full and substantial development of *** *** strategy depends on the value of P.E.,the task of teenager sport and the tough situation of competitive sport,the basic way of which is Nationwide System of P.E..That is to say,it is the choice of the *** specific way to realization of prior development of *** *** Program,increasing multiple supply of *** strengthening comprehensive training on ***.

关键词： Physical Education Teenage Sport Prior Development Education Voucher

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Chinese Herbal Medicines 2009年第1期1卷 59-65页

作者： LI Qiu-sha1, ZOU Ling-li1, FU Ting1, HAN Guo-zhu1, L Li1, ZHOU Qin1, LIN Ya-fang1, LI Nan2 1. Department of Clinical pharmacology, dalian medical university, dalian 116044, china 2. Department of Analytical Chemistry, dalian university of Science & Technology, dalian 116023, china Department of Clinical pharmacology Dalian Medical UniversityDalian 116044China Department of Analytical Chemistry Dalian University of Science & TechnologyDalian 116023China

Objective To investigate excretion profiles of the four major anti-oxidant active catechins, (-) epigallo-catechin-3-gallate (EGCG), (-) epicatechin-3-gallate (ECG), (-) epigallocatechin (EGC), and epicatechin (EC) in tea polyphenols (TP) in rats in order to provide experimental data for clinical uses and development of TP as a novel drug. Methods The above four catechins in urine, bile, and feces were simultaneously determined by high performance liquid chromatography coupled with ultraviolet absorption detector (HPLC-UV) assay with a binary gradient elution. The samples were extracted by ethyl acetate prior to HPLC. The quantification was carried out by peak area internal standard method. Following iv dosing TP 100 mg/kg to rats, the samples were collected at different time intervals up to 8 h (urine and bile) and 24 h (feces). Results The urinary Ae, 0-8 h (cumulative excretion amount over 8 h) of EGCG, ECG, EGC, and EC were, on the average, 150.83, 30.75, 116.69, and 254.56 μg, corresponding to fe, 0-8 h (cumulative excretion fraction of dose over 8 h) of 1.45%, 0.84%, 7.88%, and 10.73%, respectively; the biliary Ae, 0-8 h were 12.61, 42.64, 6.61, and 1.24 μg, corresponding to the fe, 0-8 h of 0.12%, 1.16%, 0.45%, and 0.053%,respectively. For fecal excretion, only EGCG and EGC were detected with Ae, 0-24 h of 7.38 μg (fe, 0-24 h of 0.07%) and 157 μg (fe, 0-24 h of 9.99 %), respectively. The fe, total (the total fe of 3 excretory routes) were 18.32%, 10.78%, 2.00%, and 1.64% for EGC, EC, ECG, and EGCG, respectively. Conclusion EGCG and EC are mainly excreted in urine, ECG in bile, and EGC in feces by reference to their Ae and fe. The excretion of the four catechins based on fe, total is ranked in order of EGC > EC > ECG > EGCG. Only small amount of four catechins are recovered in urine, bile, and feces, indicating an extensive metabolic conversion of catechins in the rat body.

关键词： epicatechin (-) epicatechin-3-gallate (-) epigallocatechin (-) epigallocatechin-3-gallate excretion tea polyphenols

维普期刊数据库

CYP2E1 mediated isoniazid-induced hepatotoxicity in rats

在线全文

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2004年第5期25卷 157-162页

作者： Jiang YUE, Ren-xiu PENG1, Jing YANG, Rui KONG, Juan LIUDepartment of pharmacology, medical College of Wuhan university, Wuhan 430071, china Department of pharmacology Medical College of Wuhan University

AIM: To investigate the role of CYP2E1 in isoniazid (INH)-induced hepatotoxicity and the influence of rifampicin(RFP) on INH-induced liver injury. METHODS: Rats were treated with INH alone (100 mg/kg, ip) or co-administeredwith RFP (100 mg/kg, ig) for 10 d and 21 d. Hepatotoxicity was assayed by plasma enzymes (sALT, sAST) andhistopathological examinations. Hepatic CYP2E1 activity was measured by aniline hydroxylase (ANH), and CYP2E1mRNA expression was determined by RT-PCR. Plasma hydrazine concentration was determined by ***: For a 10 d INH-treatment, hepatic CYP2E1 level was increased to 3.7-fold over the control; liverimpairment appeared after 21 d treatment, while CYP2E1 and plasma hydrazine were, respectively, increased to 4.6-fold and 1.7-fold. However, in INH-RFP group for 10 d, CYP2E1 and plasma hydrazine were, respectively,decreased by 13 % and 18 % over INH group; similarly, hepatic injury is equal to INH group appeared after 21 d,and CYP2E1 was further decreased by 26 %. Correlation analysis showed that sALT had a positive correlation withplasma hydrazine and with CYP2E1 activity; CYP2E1 activity was also markedly correlated with plasma *** compared with control, there is no difference in changes of CYP2E1 mRNA expression in INH and INH-RFPtreatment for 21 d. CONCLUSION: The metabolite of INH, hydrazine, plays an important role in INH-inducedhepatotoxicity in rats. The induction of CYP2E1 by hydrazine is involved in the hepatotoxicity of INH. RFP doesnot exacerbate INH-induced hepatotoxicity in short term, which relates to down-regulation of CYP2E1.

关键词： isoniazid rifampin hydrazines cytochrome P-450 CYP2E1