Cytoprotective effects of quercetin and its sugar-containing natural congeners in cultured HEK293 cells injured by anoxia/hypoglycemia and the struc-ture-effect relationship thereto
会议名称：《两岸三地药理学与临床药理学术会议》
会议日期：2008年
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
关 键 词：quercetin isoquercetin rutin flavonoid free-radical cytoprotection cell culture
摘 要：Objective To comparatively study anti-free radical and cytoprotective effects of quercetin(Q)and its monoglucoside isoquercetin(I),diglucoside rutin(R),which differs only in glycosyl-substitution at C-3 position of the molecules,using anoxia/hypoglycemia-induced cell injury model and thereby to explore the structure-effect relationship *** The cell injury model was established by HEK293 cells cul- tured in vitro with NaSO plus sugar-free Earle’s fluid as incubation medium;Cell survival rate(CSR), total antioxidant capacity(TAC),SOD and LDH levels were determined;The effect intensity of the 3 flavonoids were compared by means of IC,the concentration required to achieve 50% inhibition of the changes in the above indices in injured *** Q,I and R all concentration-dependently elevated CSR, TAC and SOD,and reduced LDH level;the all of IC50s for the above indices were ranked in order of ICI*** The 3 structurally similar flavoloids all have significant and concentration-dependent anti-free radical and cyto-protective effects with the intensity being in order of aglyconemonoglucosidediglucoside;the substitution of-OH by sugar group at C-3 position of flavoloids and increase in the sugar-substituent number are associated with the ef- fect intensity reduced;namely,the intensity of these effects of flavonoids is negatively related the substutu- tion by sugar group at C-3 position.