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中国生理学会第23届全国会员代表大会暨生理学学术大会

作者： Jin-Feng ZHAO,Dan WANG,Ling-Min LI,Ce ZHANG~* department of physiology,shanxi medical university,taiyuan 030001,china

Alzheimer’s disease(AD) is the most common human neurodegenerative disorder characterized by the progressive deterioration of cognition and memory in association with the presence of senile plaques(SP),neurofibrillary tangles(NFT),and massive loss of *** NFT contain paired helical filaments(PHF),which are mostly composed of hyperphosphorylated *** is a microtubule-associated protein enriched in the axonal compartment and an imbalance of protein kinases and protein phosphatases is essential for tau abnormal *** acid(OA) is an extractive of marine life which can inhibit PP2A,one of major types of protein phosphatases,cause tau hyperphosphorylation and then result in neurotoxicity.A novel peptide termed humanin(HN) was recently reported to rescue neurons in vitro from the toxicity induced by amyloid-β(Aβ) peptides and familial AD(FAD)-linked mutant *** studies in our lab have shown that HN can attenuate exitotoxicity induced by NMDA,and inhibit neuronal insults induced by hypoxia in cortical *** these results strongly suggested that HN might have a broader spectrum of protection ***,this experiment was designed to investigate whether HN could inhibit OA-induced tau hyperphosphorylation and ***: First part is to determine OA-induced phosphorylation level of tau protein and protection of HN detected by Western -blot *** showed that lower concentration of OA(5 nmol/L) has no effects,howerever,the phosphorylation level of tau protein was significantly increased when neurons were treated with OA(10 nmol/L,20 nmol/L,P202、Ser- 396 and *** neurons was pretreated with HN(5μmol/L,10μmol/L,20μmol/L) for 24 h respectively,5 umol/L HN had no effects on OA-induced hyperphosphorylation of tau(P>0.05),while the higher concentration of HN(10μmol/L,20μmol/L) significantly inhibited OA-induced hyperp

关键词： tau protein okadaic acid hyperphosphorylation neurotoxicity,humanin

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Separation and purification of embryonic rat spinal motoneurons by magnetic activated cell sorting

Separation and purification of embryonic rat spinal motoneur...

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Fei WANG,Chun-Fang WANG~* department of Biotechnology,shanxi medical university,taiyuan 030001,china

Objective To explore the experimental condition of sorting spinal motoneurons by magnetic activated cell sorting(MACS),and create a favorable condition to study the characteristics,culture and transplantation of motoneurons. Methods The ventral spinal cord cell suspensions from 16 days old rat fetus were isolated,and the motoneurons were purified by MACS and the culture system of serum-free *** motoneurons were identified by immunostaining with specific polyclonal antibody to choline acetyltransferase(ChAT).Results The present experiment revealed that the isolated and purified cells might live in *** the culture of about one week,more than 95%cells were ChAT-positive by immunofluorescent *** This study suggests a purified method of spinal motoneuron culture successfully,which insures the cells to be satisfactorily used in further research of motoneurons.

关键词： spinal cord motoneurons MACS purification rats

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Liver X receptor agonist T0901317 reduces atherosclerotic lesions in apoE^(-/-) mice by up-regulating NPC1 expression

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Science china(Life Sciences) 2008年第5期51卷 418-429页

作者： OU Xiang1,2, DAI XiaoYan1, LONG ZhiFeng3, TANG YaLing1, CAO DongLi1, HAO XinRui1, HU YanWei1, LI XiaoXu1 & TANG ChaoKe1 1 Institute of Cardiovascular Research, Key Laboratory for Atherosclerology of Hunan Province, university of South china, Heng- yang 421001, china 2 department of physiology, medical College of Shaoguan university, Shaoguan 512026, china 3 department of Histology and Embryology, university of South china, Hengyang 421001, china Institute of Cardiovascular Research Key Laboratory for Atherosclerology of Hunan Province University of South China Hengyang 421001 China department of physiology Medical College of Shaoguan University Shaoguan 512026 China department of Histology Embryology University of South China Hengyang 421001 China

In this study, we studied the effect of liver X receptor (LXR) agonist T0901317 on Niemann-Pick C1 protein (NPC1) expression in apoE-/- mice. Male apoE-/- mice were randomized into 4 groups, baseline group (n=10), control group (n=14), treatment group (n=14) and prevention group (n=14). All of the mice were fed with a high-fat/high-cholesterol (HFHC) diet containing 15% fat and 0.25% cholesterol. The baseline group treated with vehicle was sacrificed after 8 weeks of the diet. The control group and the prevention group were treated with either vehicle or T0901317 daily by oral gavage for 14 weeks. The treatment group was treated with vehicle for 8 weeks, and then was treated with the agonist T0901317 for additional 6 weeks. Gene and protein expression was analyzed by real-time quantitative PCR, immunohistochemistry and Western blotting, respectively. Plasma lipid concentrations were measured by commercially enzymatic methods. We used RNA interference technology to silence NPC1 gene expression in THP-1 macrophage-derived foam cells and then detected the effect of LXR agonist T0901317 on cholesterol efflux. Plasma triglyceride (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C) and apoA-I concentrations were markedly increased in T0901317-treated groups. T0901317 treatment reduced the aortic atherosclerotic lesion area by 64.2% in the prevention group and 58.3% in the treatment group. LXR agonist treatment increased NPC1 mRNA expression and protein levels in the small intestine, liver and aorta of apoE-/- mice. Compared with the normal cells, cholesterol efflux of siRNA THP-1 macrophage-derived foam cells was significantly decreased, whereas cholesterol efflux of LXR agonist T0901317-treated THP-1 macrophage-derived foam cells was significantly increased. Our results suggest that LXR agonist T0901317 inhibits atherosclerosis development in apoE-/- mice, which is related to up-regulating NPC1 expression.

关键词： Niemann-Pick C1 protein liver X receptor agonist atherosclerosis plaque

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Finite p-groups all of whose maximal abelian subgroups are soft

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Science china Mathematics 2010年第11期53卷 3037-3040页

作者： QU HaiPeng department of Mathematics, shanxi Normal university, Linfen 041004, china 1. department of Mathematics Shanxi Normal University Linfen 041004 China

A subgroup A of a p-group G is said to be soft in G if CG(A) = A and |NG(A)/A| = p. In this paper we determined finite p-groups all of whose maximal abelian subgroups are soft; see Theorem A and Proposition 2.4.

关键词： soft subgroup p-group of maximal class

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Val8-GLP-1（7-36）-NH2 protects against amyloid β-protein induced suppression of rat hippocampal late-phase long-term potentiation in vivo

Val8-GLP-1（7-36）-NH2 protects against amyloid β-protein i...

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Xiao-Hui WANG,Jin-Shun QI~* department of Neurobiology and National Key Discipline of physiology,shanxi medical university,taiyuan 030001, china

＜正＞Objective Alzheimer’s disease（AD） in the elderly is a major public health problem around the *** of the characteristics of AD is the presence of high density senile plaques（SPs） in the brain,especially in the learning and memory-associated *** has been well known that SPs are mainly composed of neurotoxic Amyloidβprotein （Aβ）.Interestingly,a close correlation between AD and diabetes type 2（T2DM）,another degenerative desease,has been recently ***,it was found that glucagon-like peptide 1（GLP-1） used in the treatment of T2DM was also effective in the treatment of ***,the neuroprotective action of GLP-1,especially its protection against Aβ-induced impairment of synaptic plasticity is still unknown up to *** present study is to explore the effect of Val8- GLP-1（7-36）-NH2,a proteaseresistant form of GLP-1,on the hippocampal late-phase long-term potentiation（L-LTP）,a very useful electrophysiological model for learning and memory study and further investigated whether Val8-GLP-1（7-36）-NH2 could protect against Aβ-induced suppression of hippocampal L-LTP in the CA1 region of rats in *** LTP was induced using a HFS protocol consisting of 20 pulses at 200Hz and repeating three times at an interval of 30 *** sets of HFS with 5 min of interval were applied to produce a robust L-LTP and the fEPSPs were monitored for at least 3 *** （1） intracerebraventricular（i.c.v.） injection of Val8-GLP-l（7-36）-NH2 did not affect the baseline field excitatory postsynaptic potentials（fEPSPs）,even at 16.5 ng of high concentration,but dose-dependently enhanced multiple high-frequency stimuli（HFS）-induced *** the increase of Val8-GLP-l（7-36）-NH2 concentration（0.165 ng,1.65 ng and 16.5 ng）,the values of fEPSP 3 h after HFS increased compared with control.（2） *** of Aβfragments 1-40（21.65μg） significantly impaired the maintenance of hippocampal L-LTP.（3） *** of Val8-GLP-1（7-36）-NH2（0.165 ng,1.65 ng and 16

关键词： Val8-GLP-1(7-36)-NH2 Aβ(1-40) L-LTP

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TNF-α mediates hyperalgesia and allodynia by upregulating p-NR1 expression in spinal cord in rats

TNF-α mediates hyperalgesia and allodynia by upregulating p...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Feng GAO,Xin ZHAO,Ce ZHANG,Yu ZHANG~* department of physiology,Key laboratory of cellular physiology,shanxi medical university,taiyuan 030001,china

＜正＞Objective Following the input of peripheral nociceptive information,some proinflammatory cytokines from microglia,such as TNF-α,will be released,and further produce and maintain the hyperalgesia and allodynia. However,its mechanism is not yet *** is a necessary subunit of NMDAR,and it’s well known that phosphorylation of NRl（p-NRl） can upregulate the plasticity of synaptic transmission,which is the essentials of hyperalgesia and *** consequence,the present study was undertaken to determine whether TNF-αcan mediate hyperalgesia and allodynia by upregulating spinal p-NRl expression in *** The paw withdrawal latency（PWL） test for assessing hyperalgesia / allodynia and Western blot for measuring p-NRl were implemented in the models of CFA-induced peripheral inflammation hyperalgesia or in the rats of TNF-αagonist intrathecally ***（1） Thermalgia responses and spinal p-NRl expression will be upregulated in the models of CFA-induced peripheral inflammation pain,which can be alleviated by TNF-αantagonist;（2） Intrathecal administration of TNF receptor（TNFR1） agonist will induce the development of hyperalgesia / allodynia,and enhance the expression of p-NRl in normal rats,which also can be prevented by TNF-α*** TNF-αmediates hyperalgesia and allodynia by upregulating p-NRl expression in spinal cord in rats.

关键词： TNF-α p-NR1 hyperalgesia allodynia spinal cord

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Oscillatory and Asymptotic Behaviour of Fourth Order Nonlinear Difference Equations

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Acta Mathematica Sinica,English Series 1997年第1期13卷 105-115页

作者： Yan Jurang (department of Mathematics,shanxi university,taiyuan,030006,china)Liu Bin (department of Mathematics,Hubei Normal university,Huangshi,435002,china) department of Mathematics Shanxi University 030006 Taiyuan China

This paper considers a class of fourth order nonlinear difference equations Δ2（rnΔ2yn）+ f（n,yn）=0,n∈N（n0）,where f（n,y）may be classified as superlinear,sublinear,strongly super- linear and strongly *** superlinear and sublinear cases,necessary and sufficient conditions are obtained for the difference equation to admit the existence of nonoscillatory solutions with special asymptotic *** strongly superlinear and strongly sublinear cases,sufficient conditions are given for all solutions to be oscillatory.

关键词： Nonlinear difference equation Oscillation Asymptotic behaviour.

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Isoprenaline enhances local Ca2+ release in cardiac myocytes

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Acta Pharmacologica Sinica 2006年第07期27卷 927-932页

作者： Jian-xin SHEN~(2,3,42 department of physiology,Shantou university medical College,Shantou 515041,china 3 department of physiology,medical School of Xi'an Jiaotong university,Xi'an 710061,china departmentofphysiology ShantouUniversityMedicalCollegeShantou515041China departmentofphysiology MedicalSchooloJXi'anJiaotongUniversityXi'an710061China

Aim:Contraction of cardiac myocytes is controlled by the generation and ampli-fication of intracellular Ca2+*** key step of this process is the couplingbetween sarcolemma L-type Ca2+channels（LCCs）and ryanodine receptors（RyRs）in the sarcoplasmic reticulum（SR）.β-Adrenergic stimulation is an important regu-latory mechanism for this coupling *** the details underlied the globallevel,which require local Ca2+release study are still *** present study isto explore the effects of β-adrenergic stimulation on local Ca2+***:Using confocal microscopy combined with loose-seal patch-clamp approaches,effects of isoprenaline(1 μmol·L-1),a β-adrenergic agonist,on local SR Ca2+re-lease triggered by Ca2+influx through LCCs in intact rat cardiac myocytes ***:Isoprenaline increased the intensity of ensemble averagedlocal Ca2+transients,the peak of which displayed a typical bell-shaped voltage-dependence over the membrane voltages ranging from～-40mV to～+35m ***-ther analysis showed that this enhancement could be explained by the increasedcoupling fidelity（which refers the increased probability of RyRs activation upondepolarization）,and the increased amplitude of evoked Ca2+sparks(due to moreCa2+releases through local RyRs).In addition,isoprenaline decreased the firstlatency,which displayed a typical"U"-shaped voltage-dependence,showing theavailable acceleration and synchronization of β-adrenergic stimulation on intrac-ellular calcium ***:Isoprenaline enhances local Ca2+release incardiac *** results underscore the importance of regulation of β-adrenergic stimulation on local intermolecular signals between LCCs and RyRs inheart cells.

关键词： local calcium release calcium signaling cardiac myocytes isoprenaline confocal microscopy

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Somatic/visceral sensory neurons in the spinal cord and synaptic/junctional activity in the spinal ganglion

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Chinese Science Bulletin 1998年第1期43卷 1-7页

作者： Lv Guowei department of Neurobiology, Capital university of medical Sciences, Beijing 100054, china department of Neurobiology Capital University of Medical Sciences Beijing China

Somato_visceral interaction or acupoint_visceral interaction is an important subject that greatly concerns scholars of both Chinese traditional and Western modern medicine. The role of our newly discovered spinal projection neurons and type A or C spinal ganglion neurons in convergence and integration of somato_visceral sensation is introduced. The possible challenge brought about by these related results onto some traditional concepts in neuroscience is also discussed.

关键词： spinal projection neurons spinal ganglion neurons somato_visceral sensory convergence somato_visceral sensory integration somato_visceral interaction acupoint_visceral interaction.

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p38 MAPK mediates cardiovascular and behavioral responses induced by central IL-1β and footshock in conscious rats

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Acta Pharmacologica Sinica 2004年第3期25卷 12-16页

作者： Rui-mao ZHENG,Chang-jiang ZOU,Shi-gong ZHU2 department of physiology and Pathophysiology,Health Science Center,Peking university,Beijing 100083,china department of physiology and Pathophysiology Health Science Center Peking University Beijing 100083 China

AIM: To investigate the roles of p38 mitogen-activated protein kinase (p38 MAPK) in the cardiovascular and behavioral responses induced by intracerebral ventricular injection (icv) of interleukin-1β (IL-1β) or footshock. METHODS: We examined the effects of p38 MAPK on mean artery blood pressure (mABP), heart rate (HR), and motor activity (MA) during central administration of IL-1β, or footshock after icv SB203580 (a specific inhibitor of the p38 MAPK) with Cardiovascular and Behavior Telemetry System in conscious SD rats. RESULTS: (1) IL-1β (icv) or footshock remarkably rise the mABP, and the maximal changes are (7.8±1.8) and (12.3±3.5) mmHg, respectively, which was abrogated by the pretreatment with p38 inhibitor SB203580 intracerebroventricularly. (2) Compared with icv saline group, the motor activity was significantly decreased in SB203580 group with maximal changes (-7.6±1.1) counts/min after footshock. CONCLUSION: p38 MAPK plays an important role in the pres- sor response induced by central administration of IL-1β or footshock and change of motor activity after footshock in conscious rats.

关键词： mitogen-activated protein kinase p38 interleukin-1 blood pressure motor activity

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