The co-stimulatory molecule B7-H1 ameliorated the function of Human Gingiva-Derived Mesenchymal Stem Cells through IL-10 on CIA mice model
作者单位：Sun Yat-sen University Penn State University France CNRS Georgetown University
会议名称：《第十三届全国免疫学学术大会》
会议日期：2018年
学科分类：1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 10[医学]
摘 要：Human Gingiva-derive mesenchymal Stem Cells(GMSCs) have shown anti-inflammatory and immunomodulatory effects on autoimmune and other inflammatory diseases. We have recently reported that GMSCs have strong capacity to suppress human PBMC-initiated xenogenic GVHD mice model, collagen-induced arthritis, Streptozoticin-induced T1 DM mice model, and GMSCs also ameliorated atherosclerosis in *** mechanism of suppression effect of Gingiva-derive mesenchymal Stem Cells(GMSCs) remains less clear. We herein demonstrate that co-stimulatory molecule B7-H1(PD-L1) on GMSCs exert the immune-suppressive function and results in significantly improved therapeutic effects of GMSCs on autoimmune diseases. CIA was induced with type II bovine collagen(CII) and CFA in DBA/1 J mice. GMSCs were injected I.V. into mice on day 14 after immunization. GMSCs pretreated with B1-H1 blocking antibody for 24 hours. B7-H1 high or low expression subsets were sorted by *** We showed that B7-H1+ GMSC subset had stronger suppression effects on T cells proliferation compared with B7-H1 GMSC. Additionally, the anti-inflammatory capacity of GMSCs abolished when B7-H1 was blocked in collagen induced arthritis DBA1 mice model. B7-H1 promoted migration of GMSCs, and B7-H1 ameliorated immunomodulatory function of GMSCs through upregulated STAT-3 level induced IL-10 *** findings suggest the vital effect of B7-H1 on the Gingiva-derive mesenchymal stem cells and provide a promising approach for the treatment of autoimmune diseases.