Pseudodesulfovibrio indicus ***.,***.,a piezophilic sulfate-reducing bacterium from the Indian Ocean and reclassification of four Desulfovibrio spp.
作者单位：Universite de Bretagne Occidentale(UBOUEB)Institut Universitaire Europeen de la Mer(IUEM)-UMR 6197Laboratoire de Microbiologie des Environnements Extremes(LMEE)Place Nicolas Copemic CNRSIUEM-UMR 6197Laboratoire de Microbiologie des Environnements Extremes(LMEE)Place Nicolas Copernic IfremerUMR 6197Laboratoire de Microbiologie des Environnements Extremes(LMEE)Technopole Pointe du diable School of Municipal and Environmental EngineeringHarbin Institute of Technology State Key Laboratory Breeding Base of Marine Genetic ResourcesKey Laboratory of Marine Genetic ResourcesThe Third Institute of State Oceanic Administration
会议名称：《福建省海洋学会2016年学术年会》
会议日期：2016年
学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 071005[理学-微生物学] 10[医学]
摘 要：A novel sulfate-reducing bacterium,strain J2,was isolated from a serpentinized peridotite sample from the Indian *** analysis based on 16S rRNA gene sequences showed that strain J2 clustered with the genus Desulfovibrio within the family Desulfovibrionaceae,but it showed low similarity(87.95%)to the type species Desulfovibrio desulfuricans DSM *** was most closely related to *** MSL79(96.96%),followed by *** Aspo-2(96.11%),*** C1TLV30(96.04%)and *** DSM11384(95.17%).Other available sequences shared less than 93.33%16S rRNA gene sequence *** were Gram-negative,anaerobic,motile vibrios(2-6x0.4-0.6μm).Growth was observed at salinities ranging from 0.2 to 6%(optimum 2.5%),from pH 5 to 8(optimum 6.5-7),and at temperatures between 9 and 40℃(optimum 30-35℃).Strain J2 was piezophilic,growing optimally at 10 MPa(range 0-30 MP a).Strain J2 used lactate,malate,pyruvate,formate and hydrogen as energy ***,thiosulfate,sulfite,fumarate,and nitrate were used as terminal electron *** and pyruvate were *** main fatty acids were iso-C,anteiso-C,summed feature 9(iso-Cω9c and/or C10-methyl)and *** DNA G+C content of strain J2 was 63.5 mol%.The combined genotypic and phenotypic data show that strain J2 represents a novel species of a novel genus in the family Desulfovibrionaceae,for which the name Pseudodesulfovibrio indicus ***.,*** proposed,with the type strain J2(=MCCC 1A01867=DSM 101483).We also propose the reclassification of *** as Pseudodesulfovibrio piezophilus comb,nov.,*** as *** comb,nov.,*** as *** *** *** as *** ***.