Furin controlled intracellular assembly of nanoparticles for enhanced uptake of radionuclide in living cells
会议名称：《中国化学会第28届学术年会》
会议日期：2012年
学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 090102[农学-作物遗传育种]
关 键 词：Furin Self-assembly Nanoparticle Uptake Living cell
摘 要：Protease controlled assembly of nanostructures has remained challenging for supramolecular ***,we report a condensation system which could be controlled by furin,an important protease which is associated with early development of *** designed Acetyl-Arg-Val-Arg-Arg-Cys（StBu）-Tyr（I-125）-CBT（1） for self-assembling radioactive nanoparticles（I 125-NPs） in living *** scrambled control Acetyl-Arg-Tyr（I-125）-Arg-Cys（StBu）-Arg-Val-CBT（1-scr） which can not be cleaved by furin was also prepared for study in *** furin cleavage at 100 μM,the cold form of compound 1（i.e.,1-cold） can condense to form macrocyclized dimer which self-assembles to form I-NPs with a mean diameter of 150 *** uptake indicated that more of 1 than 1-scr was uptaken by breast cancer cell ***,cells incubated with 1,1-scr,or 1-scr with 100 μM 1-scr-cold showed similar efflux curves while that of cells incubated with 1 and 100 μM of 1-cold was *** 160 min after cell efflux,17.9% of the radioactivity was retained in the cells incubated with 1 and 100 μM 1-cold while the percentages of other three groups are 4.4%,3.7%,and 3.7% *** means of intracellular assembly of radioactive nanoparticles,this provides us with a new method of furin detection or cancer early detection.