The effects of cAMP/PKA singnal pathway and TSA on proliferation of thyroid cancer
作者单位：Department of Biochemical and Molecular BiologyLiaoning Medial University
会议名称：《第三届泛环渤海（七省二市）生物化学与分子生物学会——2012年学术交流会》
会议日期：2012年
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
关 键 词：Trichostatin A Thyroid squamous cell carcinoma dbc AMP PKA CDC25B MPF
摘 要：Objective:To investigate the effects of cAMP/PKA singnal pathway and TSA on proliferation of thyroid cancer cell line ***:SW579 cells were treated using dbcAMP(0. 5,1.0,and 2.0 mmol/l),after 24h,48h,and 72h,PKA activity and proliferation of the treated cells were investigated by ELISA and MIT methods respectively;SW579 cells were treated by 1.0 mmol/L dbcAMP,50nmol/L TSA,or 1.0mmol/LdbcAMP and 50nmol/L TSA, after 48h,PKA and MPF activities of the treated cells were detected using ELISA;the mRNAs of CyclinD1,CDK4,and Rb by RT - polymerase chain reaction(PCR);the proteins of PKA, Cyclinl,CDK4 and the phosporylated RB,CDC25B - pS323,and cdc2pTyrl5 by Western ***;The PKA activity of dbcAMP - treated SW579 cells increases in a concentration - and time - dependent *** of cAMP/PKA pathway by dbcAMP arrest SW579 cells at Gl phase,reduces cells at S phase while G2 - phase cells remain unchanged or ***,Activation of cAMP/PKA pathway by dbcAMP inhibits MPF activity, downregulates CyclinD1 mRNA and protein,reduces phosphorylated Rb protein,and increase phosphorylated CDC25B and cdc2,while we have not deceted any effect on CDK4 after Activation of cAMP/PKA *** to Activation of cAMP/PKA pathway,TSA also arrest SWS79cells at G1 phase,reduces cells at S phase while G2 - phase cells remain unchanged or ***,TSA inhibits MPF activity downregulates CyclinD1 mRNA and protein,reduces phosphorylated Rb protein,and increase phosphorylated CDC25B and cdc2,while we have not deceted any effect on CDK4 after treating SW579using *** TSA activates PKA and upregulates PKA protein suggesting TSA inhibits SW579 cell growth through activation of PKA ***579 cells treated using TSA and dbcAMP are more strongly arrested at Gl phase than one treated only using dbcAMP or TSA,and cells at S and G2 are more strongly reduced than one treated only using dbcAMP or TSA demonstrating a strong synergistic ***