KIF3马达分子运送N-cadherin并对发育中的神经上皮的结构起组织作用
作者单位：北京大学生命科学学院 Department of Cell Biology and Anatomy Graduate School of Medicine University of Tokyo 7-3-1 Hongo Bunkyo-ku Tokyo 113-0033 Japan Department of Cell Biology and Anatomy Graduate School of Medicine University of Tokyo 7-3-1 Hongo Bunkyo-ku Tokyo 113-0033 Japan Department of Cell Biology and Anatomy Graduate School of Medicine University of Tokyo 7-3-1 Hongo Bunkyo-ku Tokyo 113-0033 Japan Functional Genomics Section Craniofacial Developmental Biology and Regeneration Branch National Institute of Dental and Craniofacial Research National Institutes of Health Bethesda MD 20892-4326 USA Functional Genomics Section Craniofacial Developmental Biology and Regeneration Branch National Institute of Dental and Craniofacial Research National Institutes of Health Bethesda MD 20892-4326 USA Functional Genomics Section Craniofacial Developmental Biology and Regeneration Branch National Institute of Dental and Craniofacial Research National Institutes of Health Bethesda MD 20892-4326 USA Department of Cell Biology and Anatomy Graduate School of Medicine University of Tokyo 7-3-1 Hongo Bunkyo-ku Tokyo 113-0033 Japan
会议名称：《中国细胞生物学学会2005年学术大会、青年学术研讨会》
会议日期：2005年
学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 090102[农学-作物遗传育种]
关 键 词：神经上皮 N-cadherin
摘 要：KIFs(kinesin superfamily proteins)是依赖于微管的马达分子,在细胞内承担膜性细胞器或生物大分子复合物的运输作用。KIF3是由KIF3A／KIF3B／KAP3构成的向微管正极运输膜泡的马达分子。 KAP3(kinsein superfamily-associated prctein 3)为KIF3复合体的非马达亚基,负责调节KIF3马达蛋白与其货物间的相互连接。我们利用Cre／loxP介导的条件性基因靶技术(Cre／loxP-mediated conditional gene targeting strategy),建立了KAP3条件性基因剔除小鼠(conditional knockout mouse)。发现在神经系统的发育过程中,KAP3与维持神经前体细胞分裂和细胞间粘连的平衡相关。KIF3与N-cadherin和β-catenin结合,行使post-Golgi运输N-cadherin的功能。在KAP3缺失的细胞中,N-cadherin的亚细胞定位紊乱。在条件性基因剔除小鼠的脑中,由于N-cadherin不能正常定位而导致神经上皮组织的发育异常,并形成成室管膜瘤样结构。上述结果提示KIF3／KAP3具有抑制肿瘤形成的功能,为成室管膜瘤的研究提供了动物模型。