In situ Assemblies of Bacteria and Nutrient Dynamics in Response to an Ecosystem Engineer,Marine Clam Scapharca subcrenata,in the Sediment of an Aquaculture Bioremediation System

作     者：NICHOLAUS Regan LUKWAMBE Betina YANG Wen ZHU Jinyong ZHENG Zhongming NICHOLAUS Regan;LUKWAMBE Betina;YANG Wen;ZHU Jinyong;ZHENG Zhongming

作者机构：School of Marine SciencesNingbo UniversityNingbo 315832China Department of Natural SciencesMbeya University of Science and TechnologyMbeya 53000Tanzania Department of Food Science and TechnologyUniversity of Dar es SalaamDar es Salaam 35091Tanzania 

出 版 物：《Journal of Ocean University of China》 (中国海洋大学学报（英文版）)

年 卷 期：2020年第19卷第6期

页      面：1447-1460页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 0908[农学-水产] 0707[理学-海洋科学] 0815[工学-水利工程] 0824[工学-船舶与海洋工程] 0713[理学-生态学] 

基　　金：This study was supported by the Zhejiang Public Welfare Technology Research Program of China(ZPWTP)(No.LGN18C190008) the Zhejiang Provincial Natural Science Foundation of China(No.LQ20C190003) the Ningbo Municipal Natural Science Foundation(Nos.2019A610421,2019A610443),and the K.C.Wong Magna Fund in Ningbo University. 

主　　题：Scapharca subcrenata effluents bioturbation:sediment chemistry bioremediation nutrient dynamics 

摘      要：The relationships between nutrient dynamics,microbial community,and macrofauna are important in bioremediation systems.In this study,we examined the effects of marine clam Scapharca subcrenata sedimentary activities on the microbial assemblages,benthic nutrient chemistry,and its subsequent remediation impacts on organic effluent in the sediment of an aquaculture wastewater bioremediation system.The results showed that microbial community composition differed significantly in the clam area(ANOSIM,R=0.707,P=0.037).Pyrosequencing of bacterial 16S rRNA gene revealed a total of 48 unique phyla,79 classes,107 orders,197 families,and 321 genera amongst all samples.The most dominant bacterial assemblages were Proteobacteria,Bacteroidetes,Acidobacteria,Firmicutes,Verrucomicrobia,and Actinobacteria,with Bacteroidetes and Firmicutes significantly higher in all treatment samples than control(P0.001).All dominant phyla in the list were shared across all samples and accounted for 89%(control)and 97%(treatment)of the total 16S rRNA.The nutrient flux rates from the sediments into the water(treatment group)were 51%(ammonium),88%(nitrate),77%(nitrite)and 45%(phosphate)higher,relative to the control implying increased mineralization,degradability,and mobility of the benthic nutrients.Similarly,significantly increased oxygen consumption rates were evident in the clam area signifying improved oxygen distribution within the sediment.The organic effluent contents associated with total-organic matter,carbon,nitrogen,and,phosphate were lower among the clam treatments relative to the control.Our results describe the potential roles and mechanisms contributed by marine bivalve S.subcrenata on benthic-bacterial-community assembly,nutrient balance,and effluent reduction in the sediments of aquaculture wastewaters bioremediation system.