Long non-coding RNA plasmacytoma variant translocation 1 linked to hypoxia-induced cardiomyocyte injury of H9c2 cells by targeting miR-135a-5p/forkhead box O1 axis

作     者：Jin-Juan Xu Wei-Hong Zheng Jun Wang Yuan-Yuan Chen 

作者机构：Department of General Practice MedicineThe Fifth People’s Hospital of Yuhang DistrictHangzhouZhejiang 311100China School of Life SciencesHuzhou UniversityHuzhouZhejiang 313000China Binjiang ClinicZhejiang Chinese Medical UniversityHangzhouZhejiang 310053China Department of GeriatricsHangzhou Hospital of Traditional Chinese MedicineHangzhouZhejiang 310007China 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2020年第133卷第24期

页      面：2953-2962页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：supported by a grant from the Traditional Chinese Medical Science and Technology Plan of Zhejiang Province(2019ZA040) 

主　　题：Acute myocardial infarction PVT1 miR-135a-5p FOXO1 

摘      要：Background:Myocardial infarction occurs due to insufficient(ischemia)blood supply to heart for long time;plasmacytoma variant translocation 1(PVT1)is a long non-coding RNAs(lncRNAs)involved in the pathogenesis of various diseases,including heart disease;However,few studies have explored its *** present study evaluated the effects of lncRNA PVT1 on hypoxic rat H9c2 ***:Hypoxic injury was examined by measuring cell viability and apoptosis by using cell counting kit-8 activity and flow cytometry *** expressions after hypoxia were estimated by quantitative real time polymerase chain reaction and the signaling pathway were explored by Western blot *** immunoprecipitation and luciferase reporter assays were applied to examine the interactions among *** were analyzed using t-test with one-way or two-way analysis of ***:The lncRNA PVT1 is up-regulated in hypoxia-stressed H9c2 cells and knockdown of PVT1 mitigates hypoxia-induced injury in H9c2 ***1 acts as a sponge for miR-135a-5p and knockdown of PVT1 attenuated the increased hypoxia-induced injury by up-regulating *** box O1(FOXO1)was identified as a target of miR-135a-5p,and the expression was negatively regulated by *** exploration of the underlying mechanism demonstrated that knockdown of FOXO1 reversed PVT1/miR-135a-5p mediated hypoxia-induced injury in H9c2 ***:PVT1 plays a crucial role in hypoxia-injured H9c2 cells through sponging miR-135a-5p and then positively regulating FOXO1.