Investigation of bone reconstruction using an attenuated immunogenicity xenogenic composite scaffold fabricated by 3D printing

作     者：Qiongxi Pan Chenyuan Gao Yingying Wang Yili Wang Cong Mao Quan Wang Sophia N.Economidou Dennis Douroumis Feng Wen Lay Poh Tan Huaqiong Li Qiongxi Pan;Chenyuan Gao;Yingying Wang;Yili Wang;Cong Mao;Quan Wang;Sophia N. Economidou;Dennis Douroumis;Feng Wen;Lay Poh Tan;Huaqiong Li

作者机构：School of Biomedical EngineeringSchool of Ophthalmology and Optometry and Eye HospitalWenzhou Medical UniversityWenzhou 325035Zhejiang ProvincePeople’s Republic of China Engineering Research Center of Clinical Functional Materials and Diagnosis and Treatment Devices of Zhejiang ProvinceWenzhou InstituteUniversity of Chinese Academy of SciencesWenzhou 325011Zhejiang ProvincePeople’s Republic of China Key Laboratory of Orthopedics of Zhejiang ProvinceDepartment of OrthopedicsThe Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical UniversityWenzhou 325027Zhejiang ProvincePeople’s Republic of China Medway School of PharmacyUniversity of KentMedway CampusCentral AvenueChatham MaritimeChathamKent ME44TBUK CIPERCentre for Innovation and Process Engineering ResearchKent ME44TBUK School of Chemical and Biomedical EngineeringNanyang Technological UniversitySingapore 637459Singapore School of Materials Science and EngineeringNanyang Technological UniversitySingapore 639798Singapore 

出 版 物：《Bio-Design and Manufacturing》 (生物设计与制造（英文）)

年 卷 期：2020年第3卷第4期

页      面：396-409页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 0817[工学-化学工程与技术] 08[工学] 0807[工学-动力工程及工程热物理] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 0802[工学-机械工程] 0836[工学-生物工程] 0811[工学-控制科学与工程] 

基　　金：the financial support from National Natural Science Foundation of China(81601626) Zhejiang Provincial Natural Science of Foundation of China(Y20C070010) start-up funding from Wenzhou Institute,University of Chinese Academy of Sciences(WIUCASQD2019002) Singapore MOE Tier 1 Grant RG46/18 

主　　题：Polycaprolactone 3D printing Decellularized porcine bone Cranial bone regeneration Attenuated immunogenicity 

摘      要：Bone is known to have a natural function to heal ***,if the bone damage is beyond a critical degree,intervention such as bone grafting may be *** this work,the fabrication of a novel bone scaffold composed of natural bone components and polycaprolactone(PCL)using 3D printing is put forward.α1,3-galactosyltransferase deficient pigs were used as the donor source of a *** porcine bone(DCB)with attenuated immunogenicity was used as the natural component of the scaffold with the aim to promote bone *** 3D printed DCB-PCL scaffolds combined essential advantages such as uniformity of the interconnected macropores and high porosity and enhanced compressive *** biological properties of the DCB-PCL scaffolds were evaluated by studying cell adhesion,viability,alkaline phosphatase activity and osteogenic gene expression of human bone marrow-derived mesenchymal stem *** in vitro results demonstrated that the DCB-PCL scaffolds exhibit an enhanced performance in promoting bone differentiation,which is correlated to the DCB ***,critical-sized cranial rat defects were used to assess the effect of DCB-PCL scaffolds on bone regeneration in *** results confirm that in comparison with PCL scaffolds,the DCB-PCL scaffolds can significantly improve new bone formation in cranial ***,the proposed 3D printed DCB-PCL scaffolds emerge as a promising regeneration alternative in the clinical treatment of large bone defects.