简易荧光仪共振光散射光谱法测定蛋白质的研究

作     者：王林 王照丽 李树伟 

作者机构：重庆渝西学院化学系重庆永川402168 四川师范大学化学与生命科学学院四川成都610066 

出 版 物：《化学研究与应用》 (Chemical Research and Application)

年 卷 期：2001年第13卷第5期

页      面：591-593页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 

主　　题：蛋白质 茜素红 共振光散射光谱 测定 荧光分光光度计 分析 

摘      要：The article studied the resonance light scattering of protein red alizarin with the simple fluorescence spectrophotometer *** Britton Robinson butter of 4.10,the red alizarin combined with proteins by intermolecular forces to form complexes,causing an enhance of resonance light scattering(RLS) at maximum wavelength of about *** on the enhancement of resonance light scattering a novel method for the determination of proteins using red alizarin was *** it affected by the limit of the filter of spectrophotometer the experiment used the wavelength of incident light doesn’t lie in the maximum wavelength,the method has better linear range within 0～10.0μg/ml,determination limits being 0.04μg/*** method had been used to determine total proteins in human serum samples and with accuracy and torelant to many foreign substance.