Designing sgRNAs for CRISPR-BEST base editing applications with CRISPyweb 2.0

作     者：Kai Blin Simon Shaw Yaojun Tong Tilmann Weber 

作者机构：The Novo Nordisk Foundation Center for BiosustainabilityTechnical University of DenmarkKgs.LyngbyDenmark 

出 版 物：《Synthetic and Systems Biotechnology》 (合成和系统生物技术（英文）)

年 卷 期：2020年第5卷第2期

页      面：99-102页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：This work was supported by grants from the Novo Nordisk Foundation[NNF10CC1016517 NNF15OC0016226 NNF16OC0021746] 

主　　题：CRISPR Base editor sgRNA Webserver CRISPR-BEST Genome editing 

摘      要：CRISPR/Cas9 systems are an established tool in genome *** double strand breaks caused by the standard Cas9-based knock-out techniques can be problematic in some organisms,new systems were developed that can efficiently create knock-outs without causing double strand breaks to elegantly sidestep these *** recently published CRISPR-BEST base editor system for actinobacteria is built around a C to T or A to G base *** base editing systems however require additional constraints to be considered for designing the ***,we present an updated version of the interactive CRISPy-web single guide RNA design tool https://***/that was built to support“classicalCRISPR and now also CRISPRBEST workflows.