Metabolic profiling of four synthetic stimulants,including the novel indanyl-cathinone 5-PPDi,after human hepatocyte incubation

作     者：David Fabregat-Safont Marie Mardal Juan V.Sancho Félix Hernández Kristian Linnet María Ibánez David Fabregat-Safont;Marie Mardal;Juan V. Sancho;Félix Hernández;Kristian Linnet;María Ibá(n)ez

作者机构：Research Institute for Pesticides and WaterUniversity Jaume IAvda.Sos Baynat s/n12071CastellónSpain Department of Forensic MedicineSection of Forensic ChemistryFaculty of Health and Medicinal SciencesUniversity of CopenhagenFrederik V's vej 112100KФbenhavnФDenmark Department of Occupational and Environmental MedicineUniversity Hospital of Northern NorwaySykehusvegenTromsoeNorway 

出 版 物：《Journal of Pharmaceutical Analysis》 (药物分析学报（英文版）)

年 卷 期：2020年第10卷第2期

页      面：147-156页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1002[医学-临床医学] 0817[工学-化学工程与技术] 0703[理学-化学] 10[医学] 100602[医学-中西医结合临床] 0702[理学-物理学] 

基　　金：financial support from the Ministerio de Economía y Competitividad Spain(Project CTQ2015-65603-P) from Universitat Jaume I(UJI-B2018-19) Ministerio de Educación,Cultura y Deporte in Spain for his predoctoral grant(FPU15/02033) financial support received for his research stay at the University of Copenhagen(EST17/00024)。 

主　　题：Synthetic cathinones 5-PPDi Metabolite identification In vitro metabolism Hepatocyte incubation High resolution mass spectrometry 

摘      要：Synthetic cathinones are new psychoactive substances that represent a health risk worldwide.For most of the 130 reported compounds,information about toxicology and/or metabolism is not available,which hampers their detection(and subsequent medical treatment)in intoxication cases.The principles of forensic analytical chemistry and the use of powerful analytical techniques are indispensable for stab-lishing the most appropriate biomarkers for these substances.Human metabolic fate of synthetic cathinones can be assessed by the analysis of urine and blood obtained from authentic consumers;however,this type of samples is limited and difficult to access.In this work,the metabolic behaviour of three synthetic cathinones(4-CEC,4-CPrC and 5-PPDi)and one amphetamine(3-FEA)has been evalu-ated by incubation with pooled human hepatocytes and metabolite identification has been performed by high-resolution mass spectrometry.This in vitro approach has previously shown its feasibility for obtaining excretory human metabolites.4-CEC and 3-FEA were not metabolised,and for 4-CPrC only two minor metabolites were obtained.On the contrary,for the recently reported 5-PPDi,twelve phase I metabolites were elucidated.Up to our knowledge,this is the first metabolic study of an indanyl-cathinone.Data reported in this paper will allow the detection of these synthetic stimulants in intoxi-cation cases,and will facilitate future research on the metabolic behaviour of other indanyl-based cathinones.