Photodynamic treatment of subcutaneous tumors in mice with photofrin Ⅱ and Argon ion laser

作     者：Jyh-Sheng You Dou-Mong Hau Gung-Tung Chen Hui-Feng Huang Wang-Chi Chen I-Hsin Lin 

作者机构：InstituteofRadiationBiologyNationalTsingHuaUniversityTaiwan ChineseMedicalDepartmentChinaMedicalCollegeHospitalTaichung40421Taiwan 

出 版 物：《CHINESE MEDICAL JOURNAL》 (Chinese Medical Journal)

年 卷 期：1996年第109卷第1期

页      面：83-8页

核心收录：

学科分类：13[艺术学] 1002[医学-临床医学] 

主　　题：CHINESE-HAMSTER CELLS PHOTORADIATION THERAPY MURINE MACROPHAGES HEMATOPORPHYRIN INVITRO LIGHT PHOTOSENSITIZATION INVIVO POTENTIATION PHOTOTHERAPY 

摘      要：OBJECTIVE To investigate the combined effects of photofrin (Pf) Ⅱ and laser irradiation on mice bearing subcutaneous tumors.METHODS Ehrlish ascites tumor cells (1×107) were implanted subcutaneously into the breast area of ICR mice. The tumors, when grew up to 0.55±0.15cm3, was administered of laser irradiation (514.5nm, 112.5mW/cm2 for 11.12 min with a total energy 75J/cm2) after intraperitoneal injection of Pf Ⅱ (5, 7.5, 10 and 15mg/kg body weight) once a day for three successive days.RESULTS The mortality rate (MR120) in the tumor control was 93.7% and the mean survival time (MST120) was 46.6 days. The best therapeutic effect (50% mortality rate, 53.8% tumor control rate and mean survival time 86 days) was observed in the group treated with 7.5mg of Pf Ⅱ per kg of body weight and total irradiation energy of 75J/cm2 (112.5mW/cm2 for 11.12 min) with 514.5nm argon ion laser light.CONCLUSIONS The effect on the survival rate of tumor cells and synthetic rates of DNA, RNA and protein were obviously inhibited, as the dose of pf Ⅱ and laser irradiation increased.