Profiling the mid-adult cecal microbiota associated with host healthy by using herbal formula Kang Shuai Lao Pian treated mid-adult mice

作     者：GONG Shu-Qing YE Ting-Ting WANG Mei-Xia HONG Zhu-Ping LIU Li CHEN Huan QIAN Jing GONG Shu-Qing;YE Ting-Ting;WANG Mei-Xia;HONG Zhu-Ping;LIU Li;CHEN Huan;QIAN Jing

作者机构：Pharmaceutical Informatics InstituteCollege of Pharmaceutical SciencesZhejiang UniversityHangzhou 310058China Key laboratory of Microbial Technology and Bioinformatics of Zhejiang ProvinceNMPA Key laboratory for Testing and Risk Warning of Pharmaceutical MicrobiologyZhejiang Institute of MicrobiologyHangzhou 310012China Technical CenterChiatai Qingchunbao Pharmaceutical Co.Ltd.Hangzhou 310023China 

出 版 物：《Chinese Journal of Natural Medicines》 (中国天然药物（英文版）)

年 卷 期：2020年第18卷第2期

页      面：90-102页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1005[医学-中医学] 1002[医学-临床医学] 0703[理学-化学] 10[医学] 100602[医学-中西医结合临床] 

基　　金：supported by the Science and Technology Department of Zhejiang Province,China(Nos.2018C02048 and 2018F10076) the Agricultural and Social Development Department of Hangzhou City,China(2018)。 

主　　题：Mid-adult Gut microbiota Host health Gut-brain axis Gut-metabolism axis 

摘      要：With the occurrence of aging process,decreased neuron dopamine,disrupted brown adipose tissue(BAT)remodeling and decreased butyrate level all reflect a weak host healthy in certain degree.Nevertheless,the signs of mid-adult gut microbiota,and its association with host healthy are not well understood.In current study,we deemed to illustrate the associations of age,neuron dopamine,BAT remodeling,butyrate and gut microbiota with the aid of traditional herbal formula Kang Shuai Lao Pian(KSLP),which is known for its anti-aging effect.Here,ELISA was performed to detect the production of brain dopamine,the mass of inguinal white adipose tissue versus interscapular brown adipose tissue(iWAT/iBAT)was calculated and considered as a sign of BAT remodeling,16 S rRNA gene sequencing was used to the detection of gut microbiota profiling and gas chromatography was used to measure the butyrate level in mice feces.Our results indicated mid-adult mice already present distinctive gut microbiota profiling compared with young mice,concomitant with which are the lower brain dopamine level and disrupted brown adipose remodeling.KSLP treatment improved the host healthy and regulated gut microbiota with enriched Firmicutes at the expense of Bacteroidetes,particularly increased the relative abundance of bacteria functionally related to dopamine and butyrate productions,which suggest KSLP treatment constructs a healthier gut environment.In conclusion,modulation of gut microbiota and butyrate may connectively regulate dopamine production and BAT remodeling through gut-brain axis and gut-metabolism axis.