Delivering MC3T3-E1 cells into injectable calcium phosphate cement through alginate-chitosan microcapsules for bone tissue engineering

作     者：Peng-yan QIAO Fang-fang LI Li-min DONG Tao XU Qiu-fei XIE 

作者机构：Department of ProsthodonticsPeking University School and Hospital of Stomatology Beijing Key Lab of Fine CeramicsInstitute of Nuclear and New Energy TechnologyTsinghua University Department of Preventive DentistryPeking University School and Hospital of Stomatology National Engineering Laboratory for Digital and Material Technology of StomatologyPeking University School and Hospital of Stomatology 

出 版 物：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报（英文版）B辑（生物医学与生物技术）)

年 卷 期：2014年第15卷第4期

页      面：382-392页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1001[医学-基础医学(可授医学、理学学位)] 0905[农学-畜牧学] 08[工学] 0906[农学-兽医学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 

基　　金：supported by the National Natural Science Foundation of China(No.30772447) the Talent Introduction Project of Peking University Health Science Center(No.bmu2009139),China 

主　　题：Injectable scaffold Calcium phosphate cement Osteoblast Microencapsulation Cell release Chitosan 

摘      要：Objective： To deliver cells deep into injectable calcium phosphate cement（CPC） through alginate-chitosan（AC） microcapsules and investigate the biological behavior of the cells released from microcapsules into the ***： Mouse osteoblastic MC3T3-E1 cells were embedded in alginate and AC microcapsules using an electrostatic droplet *** two types of cell-encapsulating microcapsules were then mixed with a CPC ***3T3-E1 cell viability was investigated using a Wst-8 kit,and osteogenic differentiation was demonstrated by an alkaline phosphatase（ALP） activity *** attachment in CPC was observed by an environment scanning electron ***： Both alginate and AC microcapsules were able to release the encapsulated MC3T3-E1 cells when mixed with CPC *** released cells attached to the setting CPC scaffolds,survived,differentiated,and formed mineralized *** grew in the pores concomitantly created by the AC microcapsules in situ within the *** Day 21,cellular ALP activity in the AC group was approximately four times that at Day 7 and exceeded that of the alginate microcapsule group（P0.05）.Pores formed by the AC microcapsules had a diameter of several hundred microns and were spherical compared with those formed by alginate ***： AC microcapsule is a promising carrier to release seeding cells deep into an injectable CPC scaffold for bone engineering.