Neutrophil extracellular traps mediate the crosstalk between glioma progression and the tumor microenvironment via the HMGB1/RAGE/IL-8 axis

作     者：Caijun Zha Xiangqi Meng Lulu Li Shan Mi Da Qian Ziwei Li Pengfei Wu Shaoshan Hu Shihong Zhao Jinquan Cai Yanhong Liu Caijun Zha;Xiangqi Meng;Lulu Li;Shan Mi;Da Qian;Ziwei Li;Pengfei Wu;Shaoshan Hu;Shihong Zhao;Jinquan Cai;Yanhong Liu

作者机构：Department of Laboratory DiagnosisThe Second Affiliated Hospital of Harbin Medical UniversityHarbin 150086China Department of NeurosurgeryThe Second Affiliated Hospital of Harbin Medical UniversityHarbin 150086China Neuroscience InstituteHeilongjiang Academy of Medical SciencesHarbin 150086China 

出 版 物：《Cancer Biology & Medicine》 (癌症生物学与医学（英文版）)

年 卷 期：2020年第17卷第1期

页      面：154-168页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by The National Natural Science Foundation of China(Grant No.81702972,Grant No.81874204) China Postdoctoral Science Foundation(Grant No.2018M640305,Grant No.2019M660074) The Research Project of the Chinese Society of Neuro-oncology,CACA(Grant No.CSNO-2016-MSD12) Heilongjiang Postdoctoral Science Foundation(Grant No.LBH-Z18103) The Research Project of the Health and Family Planning Commission of Heilongjiang Province(Grant No.2017–201) Postgraduate Research&Practice Innovation Program of Harbin Medical University(Grant No.YJSKYCX2018-94HYD) The Young and middle-aged Science Foundation of Harbin Medical University(Grant No.KYCX2018-08) 

主　　题：Neutrophil extracellular traps HMGB1 IL-8 NF-κB glioma microenvironment 

摘      要：Objective:Neutrophil extracellular traps(NETs)produced by tumor-infiltrating neutrophils(TINs)are associated with poor prognosis in patients with several types of ***,the mechanisms underlying the involvement of NETs in glioma progression remain largely *** study aimed to elucidate the roles of NETs in biological processes that drive the crosstalk between glioma progression and the tumor ***:Neutrophil infiltration and NETs formation were investigated in glioma tissue through immunohistochemistry,and their relationships with clinicopathological features and outcomes were statistically *** effects of NETs on glioma cell progression were studied in a co-culture *** vivo and in vitro experiments validated the reactive oxygen species activity and cytokine production of TINs,as well as the ERK signaling pathway activation and the metastasis of ***:Neutrophil infiltration and NETs formation were induced in high-grade glioma compared with low-grade *** induced by TINs were determined to be an oncogenic marker of high-grade gliomas and to be involved in cell proliferation and *** overproduction promoted glioma cell proliferation,migration,and ***,HMGB1 was found to bind to RAGE and activate the NF-κB signaling pathway in *** addition,NETs stimulated the NF-κB signaling pathway,thus promoting IL-8 secretion in ***,IL-8 recruited neutrophils which in turn mediated NETs formation via the PI3 K/AKT/ROS axis in ***:Our results suggest that NETs produced by TINs mediate the crosstalk between glioma progression and the tumor microenvironment by regulating the HMGB1/RAGE/IL-8 *** NETs formation or IL-8 secretion may be an effective approach to inhibit glioma progression.