Identification of target genes of transcription factor CEBPB in acute promyelocytic leukemia cells induced by all-trans retinoic acid

作     者：Lei Yu Yang-De Zhang Jun Zhou De-Ming Yao Xiang Li 

作者机构：Department of Hepatic SurgeryNational Hepatobiliary and Enteric Surgery Research CenterMinistry of HealthCentral South UniversityChina 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2013年第6卷第6期

页      面：473-480页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Chromatin immunoprecipitation All-trans retinoic acid CEBP Granulopoiesis Neutrophils 

摘      要：Objective:To indentify target genes of transcription factor CCA AT enhancer-binding protein P(CEBPB) in acute proinyelocytie leukemia cells induced by all-tram retinoie ***: A new strategy for high—throughput identification of direct target genes was established by combining chromatin immunoprecipitation(ChIP) with in vitro ***,106 potential CKBPB binding fragments from the genome of the all-trans retinoie acid(ATRA)-treated NB4 cells were ***:Of them,82 were mapped in proximity to known or previously predicted genes;7 were randomly picked up for further confirmation by ChlP-PCR and 3 genes (CALM,1TPR2 and 0RM2) were found to be specificaUy up-regulated in the ATRA-treated NB4 cells,indicating that they might lie the down-stream target genes of ***:Our results provided new insight into the mechanisms of ATRA-induced granulocytic differentiation.