Residual impact of 17α-methyltestosterone and histopathological changes in sex-reversed Nile tilapia (Oreochromis niloticus)
作者机构：Study Programme of Biotechnology of Fisheries and MarineUniversitas AirlanggaSurabayaIndonesia Department of Veterinary AnatomyFaculty of Veterinary MedicineUniversitas AirlanggaSurabayaIndonesia Department of Feed and NutritionFaculty of Veterinary MedicineUniversitas AirlanggaSurabayaIndonesia Department of Fish Health Management and AquacultureFaculty of Fisheries and MarineUniversitas AirlanggaSurabayaIndonesia Department of AquacultureBogor Agricultural University(IPB)BogorIndonesia
出 版 物：《Asian pacific Journal of Reproduction》 (亚太生殖杂志（英文版）)
年 卷 期：2020年第9卷第1期
页 面：37-43页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：17α-methyltestosterone Residue Organ histopathology Tilapia Sex reversal method
摘 要：Objective:To examine sex reversal both by oral and by immersion using 17α-methyltestosterone on the methyltestosterone residual concentration and the organ histopathology of tilapia ***:This study used oral and immersion treatment methods for sex reversal of tilapia fish and used normal fish as the control and each treatment was repeated 4 times.17α-methyltestosterone at dosages of 60 mg/kg feed and 0.5 mg/L were used for oral and immersion methods,*** the first step,tilapia fry were reared at 100 L aquaria,with a density of 1 fish/L for 2 *** the next step,male tilapias were reared at happa(net cage)of(2×1×1)m3 size in the controlled pond,with a density of 30 fish/happa for 3 *** methyltestosterone residual concentrations were analyzed by one-way analysis of variance and Duncan s multiple range tests,while organ histopathology was analyzed by descriptive ***:Residual concentrations in the serum of methyltestosterone-treated fish were significantly lower than that in normal fish,especially in 4-and 5-month-old tilapias with averages of less than 5μg/L,while in normal fish was more than 5μg/*** the flesh,methyltestosterone residual concentrations showed relatively no significant differences between the oral and immersion treatment groups and methyltestosterone-treated fish remained lower compared to normal fish,except in 5-month-old ***-treated tilapia exhibited histopathological changes on gill,liver,kidneys,and intestine ***:Sex reversal either by oral or by immersion has methyltestosterone residual concentration,but does not exceed the limits(5μg/L or 5μg/kg)of synthetic steroid on the fish body,although methyltestosterone causes histopathological changes on gill,liver,kidneys,and intestine.
维普期刊数据库