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Residual impact of 17α-methyltestosterone and histopathological changes in sex-reversed Nile tilapia (Oreochromis niloticus)

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Asian pacific Journal of Reproduction 2020年第1期9卷 37-43页

作者： Dewi Nurmalita Suseno Epy Muhammad Luqman Mirni Lamid Akhmad Taufiq Mukti Muhammad Agus Suprayudi Study Programme of Biotechnology of Fisheries and Marine Universitas AirlanggaSurabayaIndonesia Department of Veterinary Anatomy Faculty of Veterinary MedicineUniversitas AirlanggaSurabayaIndonesia Department of Feed and Nutrition Faculty of Veterinary MedicineUniversitas AirlanggaSurabayaIndonesia Department of Fish Health Management and Aquaculture Faculty of Fisheries and MarineUniversitas AirlanggaSurabayaIndonesia Department of Aquaculture Bogor Agricultural University(IPB)BogorIndonesia

Objective:To examine sex reversal both by oral and by immersion using 17α-methyltestosterone on the methyltestosterone residual concentration and the organ histopathology of tilapia ***:This study used oral and immersion treatment methods for sex reversal of tilapia fish and used normal fish as the control and each treatment was repeated 4 times.17α-methyltestosterone at dosages of 60 mg/kg feed and 0.5 mg/L were used for oral and immersion methods,*** the first step,tilapia fry were reared at 100 L aquaria,with a density of 1 fish/L for 2 *** the next step,male tilapias were reared at happa(net cage)of(2×1×1)m3 size in the controlled pond,with a density of 30 fish/happa for 3 *** methyltestosterone residual concentrations were analyzed by one-way analysis of variance and Duncan's multiple range tests,while organ histopathology was analyzed by descriptive ***:Residual concentrations in the serum of methyltestosterone-treated fish were significantly lower than that in normal fish,especially in 4-and 5-month-old tilapias with averages of less than 5μg/L,while in normal fish was more than 5μg/*** the flesh,methyltestosterone residual concentrations showed relatively no significant differences between the oral and immersion treatment groups and methyltestosterone-treated fish remained lower compared to normal fish,except in 5-month-old ***-treated tilapia exhibited histopathological changes on gill,liver,kidneys,and intestine ***:Sex reversal either by oral or by immersion has methyltestosterone residual concentration,but does not exceed the limits(5μg/L or 5μg/kg)of synthetic steroid on the fish body,although methyltestosterone causes histopathological changes on gill,liver,kidneys,and intestine.

关键词： 17α-methyltestosterone Residue Organ histopathology Tilapia Sex reversal method

Sexual Reversal with 17α-methyltestosterone in Oreochromis sp.:Comparison between Recirculation Aquaculture System(RAS)and Biofloc Technology(BFT)

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Journal of Agricultural Science and Technology(A) 2019年第2期9卷 131-139页

作者： Carlos Arturo David-Ruales Eliana Marcela Betancur-Gonzalez Ruben Dario Valbuena-Villareal Animal Science Program Corporación Universitaria LasallistaCaldas 0554Colombia Department of Exact and Natural Sciences Universidad Surcolombiana4100Colombia

Precocity in tilapia implies the use of several methods of obtaining monosex seed;the most common tends to use masculinizing hormone 17α-methyltestosterone(17αMT),with variable *** the objective of this study was to compare the efficiency of the sexual reversion process using 17αMT,in a recirculation system and in *** a totally randomized design,three tanks for recirculation(T-RAS)and three tanks for biofloc(T-BIO)with a capacity of 200 L effective volume were taken and filled with 1,056 larvae of Oreochromis sp.,without reversing and with an initial weight of 0.02 g and an initial total length of 1.4 *** study was carried out during 65 d,the fish were fed(10%biomass,adjusted every 15 d)by a commercial diet at 45%of crude protein that included 17αMT(60 mg/kg).Water quality,microbiology,zootechnical and gonadal analysis were *** the water quality results showed that just dissolved oxygen(DO),temperature(T-°C)and alkalinity did not show significant ***,in the productive parameters there were significant differences in the final length,the gain in length and in K which were better in *** microbiological ones did not present significant differences between the ***,the percentage of reversion was significantly better in ***,this study suggests that settleable solids concentrations above 35 cm decrease the efficiency of the sexual reversion for this species.

关键词： Oreochromis sp. biofloc recirculation aquaculture system 17α-methyltestosterone

Determination of 17<i>α</i>-methyltestosterone in Freshwater Samples of Tilapia Farming by High Performance Liquid Chromatography

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American Journal of Analytical Chemistry 2013年第4期4卷 207-211页

作者： Isabel R. Barbosa Sara Lopes Rhaul Oliveira Inês Domingues Amadeu M. V. M. Soares António J. A. Nogueira Centre for Pharmaceutical Studies Faculty of Pharmacy University of Coimbra Coimbra Portugal Department of Biology & CESAM University of Aveiro Aveiro Portugal Faculty of Pharmacy University of Coimbra Coimbra Portugal

17α-methyltestosterone is used to induce the sex reversal of Tilapia sp. to obtain cultures mono-sex to an economically viable. This practice may lead to environmental contamination and problems in human health. Therefore methods need to be developed to detect residues of 17α-methyltestosterone in aqueous matrices. A simple high-performance liquid chromatographic method using ultraviolet detection (245 nm) and testosterone as internal standard has been developed for the monitoring 17α-methyltestosterone in freshwater samples of tilapia aquaculture. The method described involves limited sample preparation as it includes a filtration followed by a single solid-phase extraction step using C18 cartridge. Validation data indicated that the HPLC-UV method for 17α-methyltestosterone determination in the concentration range of 50 - 2000 μg/L provided good linearity, sensitivity, accuracy and precision. Method performance was efficiently applied to monitoring the freshwater samples of fish ponds and the surrounding aquatic channels.

关键词： 17α-methyltestosterone High Performance Liquid Chromatography Environment Tilapia Farming

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17α-甲基睾酮对赤点石斑鱼性逆转的影响

水产学报 1992年第2期16卷 171-174页

作者：方永强林秋明齐襄洪桂英国家海洋局第三海洋研究所国家海洋局第三海洋研究所厦门 361005

赤点石斑鱼Epinephelus akaara(Temminck et Schlegel)是我国名贵海产经济鱼类。近几年来,因盲目钩捕,在东南沿海资源日益减少。因此,国内外一些学者对赤点石斑鱼性腺发育进行周年研究,并开展了人工繁殖和育苗试验。然而,由于此鱼雌雄同... 详细信息

赤点石斑鱼Epinephelus akaara(Temminck et Schlegel)是我国名贵海产经济鱼类。近几年来,因盲目钩捕,在东南沿海资源日益减少。因此,国内外一些学者对赤点石斑鱼性腺发育进行周年研究,并开展了人工繁殖和育苗试验。然而,由于此鱼雌雄同体,雌性先熟,在六龄鱼才转变为雄鱼。由此常见因缺少雄鱼无法开展人工繁殖。所以,作者在非繁殖季节,试图用外源性雄激素拌入饵料中,喂低龄和高龄赤点石斑鱼,目的在于诱发其性逆转,现已取得显著的效果,这对于解决雄鱼困难,提供科学依据。

关键词： Epinephelus akaara 17α-methyltestosterone hermaphrodite spermatogenesis