Comparative Ligandomic Analysis of Human Lung Epithelial Cells Exposed to PM2.5

作     者：TIAN Hong Akhalesh SHAKYA WANG Feng WU Wei Dong LI Wei TIAN Hong;Akhalesh SHAKYA;WANG Feng;WU Wei Dong;LI Wei

作者机构：School of Public HealthXinxiang Medical UniversityXinxiang 453003HenanChina Bascom Palmer Eye InstituteDepartment of OphthalmologyUniversity of Miami School of MedicineMiami 33136FloridaUSA Department of Genomic MedicineUT MD Anderson Cancer CenterHouston 77054TXUSA 

出 版 物：《Biomedical and Environmental Sciences》 (生物医学与环境科学（英文版）)

年 卷 期：2020年第33卷第3期

页      面：165-173页

核心收录：

学科分类：100405[医学-卫生毒理学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

基　　金：supported by the National Institute of Health under Grant#R01EY027749-01A1,#R24EY028764-01A1,#R21EY027065-02,#R41EY027665-01A1 to LW,and#P30-EY014801 The American Diabetes Association under Grant#1-18-IBS-172 to LW An institutional grant from Research to Prevent Blindness The National Natural Science Foundation of China under Grant#81573112 and#81373030 to WWD 

主　　题：PM2.5 Ligandomics Lung epithelial cell Comparative ligandomics Ligand 

摘      要：Objective To investigate whether exposure to particulate matter of diameter equal to or less than 2.5μm(PM2.5)alters the response of lung epithelial cells to extrinsic regulation by globally profiling cell surface ligands and quantifying their binding *** Human A549 lung epithelial cells(LECs)were treated with or without *** profiling was applied to these cells for the global identification of LEC-binding ligands with simultaneous quantification of binding *** comparisons of the entire ligandome profiles systematically identified ligands with increased or decreased binding to PM2.5-treated *** We found 143 ligands with increased binding to PM2.5-treated LECs and 404 ligands with decreased *** other ligands showed no change in binding *** example,apolipoprotein E(ApoE),Notch2,and growth arrest-specific 6(Gas6)represent ligands with increased,decreased,or unchanged binding activity,*** ApoE and Gas6 are phagocytosis ligands,suggesting that phagocytic receptors on LECs after stimulation with PM2.5 were differentially upregulated by *** These results suggest that the newly-developed ligandomics is a valuable approach to globally profile the response of LECs to PM2.5 in terms of regulating the expression of cell surface receptors,as quantified by ligand binding *** quantitative ligandome profiling will provide indepth understanding of the LEC molecular response on the cell surface to particulate matter air pollution.