On-Line Photoinduced Fluorescence for Carbamazepine Determination after Multivariate Optimization
On-Line Photoinduced Fluorescence for Carbamazepine Determination after Multivariate Optimization作者机构：Área de Farmacotecnia Ética y Legislación FarmacÉutica San Luis Argentina Instituto de Química de San Luis (INQUISAL-CONICET) San Luis Argentina Área de Gestión en Calidad y Salud San Luis Argentina Área de Química Analítica Facultad de Química Bioquímica y Farmacia Universidad Nacional de San Luis Chacabuco y Pedernera San Luis Argentina
出 版 物：《American Journal of Analytical Chemistry》 (美国分析化学（英文）)
年 卷 期：2019年第10卷第11期
页 面：513-527页
学科分类：081704[工学-应用化学] 07[理学] 070304[理学-物理化学(含∶化学物理)] 08[工学] 0817[工学-化学工程与技术] 0703[理学-化学]
主 题：Online Photoinduced Fluorescence Multivariate Optimization Carbamazepine Pharmaceutical Samples
摘 要：This work presents a simple and sensitive flow injection fluorimetric method for the determination of carbamazepine (CBZ) in pharmaceutical samples. It is based on the fluorescence signal measurement of the product generated by photoinduced (λex = 251 nm;λem = 434 nm) using a flow injection manifold containing an online homemade photoreactor. In order to optimize the experimental setup, the variable conditions were studied using a multivariate optimization method, thus finding the set of optimum parameters according to the evaluated responses (sensitivity and sampling frequency) and minimizing the number of experiments performed. Under optimal experimental conditions, linear relationship with good correlation coefficient (0.9956) was found between the fluorescence intensity and CBZ concentration in the range of 0.13 - 40 μg·mL-1. The limit of detection was 0.04 μg·mL-1. The precision of the method was satisfactory;the values of relative standard deviations were better than 4.36%. The proposed method was validated and successfully applied to the determination of CBZ in pharmaceuticals with good accuracy;being the recovery values from 89.45% - 112.20%.
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