Epigallocatechin Gallate-Stearate Enhances the Efficacy of Antibiotics
Epigallocatechin Gallate-Stearate Enhances the Efficacy of Antibiotics作者机构：Department of Biological Sciences Seton Hall University South Orange NJ USA Department of Biology Montclair State University Montclair NJ USA
出 版 物：《Open Journal of Medical Microbiology》 (医学微生物学（英文）)
年 卷 期：2019年第9卷第3期
页 面：77-94页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：EGCG EGCG-Stearate Antibiotic Resistance Disk Diffusion Methods
摘 要：Introduction: The rise in antibiotic resistant cases has caused a global concern. Researchers around the world are trying to find a novel alternative to combat this issue. Green tea with its many health benefits, including antibacterial and antiviral activity, has shown to be one of the most promising candidates to be used as an agent to solve this problem. Objective: This study focuses on evaluating the synergistic effects of antibiotics and two green tea polyphenols: epigallocatechin gallate (EGCG), and its modified lipophilic form epigallocatechin gallate stearate (EGCG-S). Methods: In this study, twelve antibiotics and eight bacteria: Gram-positive Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis) and Bacillus megaterium (B. megaterium);Gram-negative Escherichia coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa), Serratia marcescens (S. marcescens), and Enterobacter aerogenes (E. aerogenes);and acid-fast Mycobacterium smegmatis (M. smegmatis) were used. Antibacterial synergism profiling of EGCG, EGCG-S and antibiotics has been established using a disk diffusion assay. Results: The results revealed that both 1% of EGCG and 1% EGCG-S enhanced the antimicrobial activities on antibiotics in various bacteria. Antimicrobial susceptibility study indicated that EGCG-S was able to enhance some antibiotics from the resistant category to intermediate or susceptible and/or from intermediate category to susceptible. Both EGCG and EGCG-S worked comparably on Gram-positive bacteria;in S. aureus, both compounds enhanced 5 antibiotics (AM10, CF30, C30, S10 and TE30) activities while EGCG-S had higher efficiency. B. megaterium were susceptible to most of the antibiotic treatment, thus the impact of EGCG and EGCG-S was insignificant. EGCG-S worked better than EGCG on Gram-negative bacteria;converted 9 antibiotics susceptibility in E. coli and P. aeruginosa, and 8 antibiotics in E. aerogenes. EGCG and EGCG-S also showed synergism on acid-fast bacter
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