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Inhibition of Herpes Simplex Virus-1 by the Modified Green Tea Polyphenol egcg-stearate

Advances in Bioscience and Biotechnology 2018年第12期9卷 679-690页

作者： Shivani N. Patel Sandra D. Adams Lee H. Lee Department of Biology Montclair State University Montclair NJ USA

Epigallocatechin gallate (egcg), a green tea polyphenol possesses antioxidant, antibacterial, anticancer and antiviral properties. egcg-stearate (egcg-S) is of interest for this study because of its stability and lipophilic properties. The chemical modification of egcg-S increased its lipid solubility. Herpes simplex virus-1 (HSV-1), a member of the family Herpesviridae, and Alphaherpesvirinae subfamily is a leading cause of human viral diseases in the United States. In this study, 25 μM, 50 μM, 75 μM, and 100 μM of egcg and egcg-S were used to carry out cytotoxicity, cell viability and cell proliferation assays to determine the maximum non-cytotoxic concentrations on cultured A549 cells. The results suggested that 75 μM of egcg and egcg-S is the appropriate concentration to further study the effect on the infection of HSV-1 in A549 cells. Infectivity, antiviral, and inverted microscopy assays were performed to study the effects of egcg and egcg-S on HSV-1 infection. An antiviral assay was performed using luminescence and it indicated that egcg-S treated HSV-1 showed up to 90% inhibition. Confocal microscopy images further supported the inhibitory effects of 75 μM egcg-S on HSV-1 infection in A549 cells. The long-term goal of this research is to use egcg-S as a possible novel topical therapeutic treatment to limit the spread of HSV-1 infections.

关键词： HSV-1 egcg-stearate egcg Antiviral A549 Cells

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Epigallocatechin Gallate-stearate Enhances the Efficacy of Antibiotics

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Open Journal of Medical Microbiology 2019年第3期9卷 77-94页

作者： Ayuni Yussof Umme Habiba Deborah Liaw Tinchun Chu Lee H. Lee Department of Biological Sciences Seton Hall University South Orange NJ USA Department of Biology Montclair State University Montclair NJ USA

Introduction: The rise in antibiotic resistant cases has caused a global concern. Researchers around the world are trying to find a novel alternative to combat this issue. Green tea with its many health benefits, including antibacterial and antiviral activity, has shown to be one of the most promising candidates to be used as an agent to solve this problem. Objective: This study focuses on evaluating the synergistic effects of antibiotics and two green tea polyphenols: epigallocatechin gallate (egcg), and its modified lipophilic form epigallocatechin gallate stearate (egcg-S). Methods: In this study, twelve antibiotics and eight bacteria: Gram-positive Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis) and Bacillus megaterium (B. megaterium);Gram-negative Escherichia coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa), Serratia marcescens (S. marcescens), and Enterobacter aerogenes (E. aerogenes);and acid-fast Mycobacterium smegmatis (M. smegmatis) were used. Antibacterial synergism profiling of egcg, egcg-S and antibiotics has been established using a disk diffusion assay. Results: The results revealed that both 1% of egcg and 1% egcg-S enhanced the antimicrobial activities on antibiotics in various bacteria. Antimicrobial susceptibility study indicated that egcg-S was able to enhance some antibiotics from the resistant category to intermediate or susceptible and/or from intermediate category to susceptible. Both egcg and egcg-S worked comparably on Gram-positive bacteria;in S. aureus, both compounds enhanced 5 antibiotics (AM10, CF30, C30, S10 and TE30) activities while egcg-S had higher efficiency. B. megaterium were susceptible to most of the antibiotic treatment, thus the impact of egcg and egcg-S was insignificant. egcg-S worked better than egcg on Gram-negative bacteria;converted 9 antibiotics susceptibility in E. coli and P. aeruginosa, and 8 antibiotics in E. aerogenes. egcg and egcg-S also showed synergism on acid-fast bacter

关键词： egcg egcg-stearate Antibiotic Resistance Disk Diffusion Methods

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