Concentrated Extract of Green Tea Polyphenols Enhances the Toxicity of the Elderberry Lectin Nigrin b to Mice

作     者：Pilar Jiménez Patricia Cabrero Jesús Tejero Manuel J. Gayoso Manuel Garrosa Damián Cordoba-Diaz Manuel Cordoba-Diaz Tomás Girbés 

作者机构：Biología Celular Histología y Farmacología Facul- tad de Medicina and INCYL (Instituto de Neurociencias de Castilla y León) Universidad de Valladolid Valladolid Spain Farmacia y Tecnología Farmacéutica Facultad de Farmacia and IUFI (Instituto Universitario de Farmacia Industrial) Universidad Complu- tense de Madrid Madrid Spain Nutrición y Bromatología Facultad de Medicina and CINAD (Centro de Investigación en Nutrición Alimentación y Dietética Edi- ficio LUCIA-Parque Científico) Universidad de Valladolid Valladolid Spain Nutrición y Bromatología Facultad de Medicina and CINAD (Centro de Investigación en Nutrición Alimentación y Dietética Edi- ficio LUCIA-Parque Científico) Universidad de Valladolid Valladolid Spain 

出 版 物：《Food and Nutrition Sciences》 (食品与营养科学（英文）)

年 卷 期：2014年第5卷第5期

页      面：466-471页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Green Tea Polyphenols Nigrin b Model Ribosome-Inactivating Protein Lectin Small Intestine Liver Apoptosis 

摘      要：The effect of the administration of large amounts of green tea polyphenols is a matter of controversy. We explored whether a polyphenol mixture from a concentrated green tea extract (Polyphenon 60) could alter the effects on mice of the type 2 (two chains) ribosome-inactivating protein nigrin b isolated from Sambucus nigra L. Nigrin b triggers specific reversible toxic effects on the mouse intestines featured by apoptosis of mice Lieberkühn crypt cells upon parenteral administration of sub-lethal amounts. Independent administration to mice of 30 mg/kg body weight of Polyphenon 60 by oral gavage or 10 mg/kg body weight of nigrin b administered via the intraperitoneal route (i.p.) did not affect survival. In contrast, the simultaneous treatment greatly enhanced nigrin b toxicity leading to the death of some animals. The histological analysis revealed that the most serious injury was inflicted on the small intestine crypts, which disappeared, and on the liver, which evidenced hepatotoxicity showing haemorrhagic areas. These findings raise concerns about the abuse of high concentrations of green tea polyphenols especially when the intestinal mucosa is damaged, for instance by toxins or therapeutic drugs.