Differential Anticancer Effect of an Apple Extract (Applephenon<sup>&reg</sup>), Polyphenols and Isoflavones on Normal Human Keratinocytes and Epidermoid Cancer Cells

作     者：John J. Wille Mark A. Berhow Jong Y. Park 

作者机构：Bioplast Medical LLC Chesterfield NJ USA ARS USDA Peoria IL USA Moffitt Cancer Center Tampa FL USA 

出 版 物：《Journal of Cancer Therapy》 (癌症治疗（英文）)

年 卷 期：2019年第10卷第6期

页      面：476-493页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：ApplephenonTM (AP-SH Grade) Cytotoxicity Epicatechin Epidermal Keratinocytes Epidermoid Cancer Cell Lines (A431 HaCaT HeLa) Genistein Polyphenols 

摘      要：Applephenon®, a purified extract prepared from green apples, was examined for its cytotoxicity and inhibitory effects on the proliferation of cultures of normal human keratinocytes and several epidermoid cancer cell lines. Our HPLC studies demonstrated a high content of phenolic compounds (65%), including catechin, epicatechin, caffeic acid and phloretin as well as polyphenols such as proanthocyanidins. Applephenon® demonstrated a greater cytotoxic effect against HeLa, A431 cancer cell lines and HaCaT, an immortalized keratinocyte cell line than serum-free cultures of proliferating normal human keratinocytes (NHK). Proliferation of NHK was inhibited at concentrations above 0.0013% while concentrations above 0.005% were cytotoxic. By contrast, Applephenon® solutions above 0.00025% killed each of the cancer cell lines. Treated cells displayed increased intercellular separation and evidence of keratinizing stratification. We also tested the effect of epicatechin, and two isoflavonoids, genistein and daidzein, on cancer cell lines. Hela cells were more sensitive to epicatechin and genistein inhibition of cell growth and cytotoxicity than were NHK. Daidzein at these concentrations had little effect on cancer cells. These results indicate that Applephenon® and some of its phenolic components have selective anticancer activity.