In Search of Regulators of <i>LeSPL-CNR</i>by South-Western Blotting and Yeast One-Hybrid Library Screening System
In Search of Regulators of <i>LeSPL-CNR</i>by South-Western Blotting and Yeast One-Hybrid Library Screening System作者机构：Research Centre for Plant RNA Signaling College of Life and Environmental Sciences Hangzhou Normal University Hangzhou China Key Laboratory for Quality and Safety of Agricultural Products of Hangzhou City College of Life and Environmental Science Hangzhou Normal University Hangzhou China
出 版 物：《American Journal of Plant Sciences》 (美国植物学期刊（英文）)
年 卷 期：2018年第9卷第5期
页 面：1037-1050页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：Solanum lycopersicum LeSPL-CNR 286 bp Region South-Western Blotting Yeast One-Hybrid
摘 要：LeSPL-CNR is a crucial transcription factor for fruit ripening of Solanum lycopersicum. The cnr (colorless non-ripening) epimutation resulted from hypermethylation in a 286 bp region of LeSPL-CNR promoter inhibits normal fruit ripening. In present study, potential regulators of LeSPL-CNR, which could bind to the specific 286 bp region, were screened via south-western blotting and yeast one-hybrid (Y1H) library screening system. Results indicated that a total of 13 and 19 candidate proteins were acquired respectively, and both ribulose-1,5-bisphosphate carboxylase/oxygenase and 40S ribosomal protein were identified by two methods. These would provide some information for revealing roles of DNA methylation and the regulatory mechanism for LeSPL-CNR.
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