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In Search of Regulators of <i>LeSPL-CNR</i>by south-western Blotting and Yeast One-Hybrid Library Screening System

American Journal of Plant Sciences 2018年第5期9卷 1037-1050页

作者： Xiaohong Wang Bishun Ye Ying Wang Ting Zhou Tongfei Lai Research Centre for Plant RNA Signaling College of Life and Environmental Sciences Hangzhou Normal University Hangzhou China Key Laboratory for Quality and Safety of Agricultural Products of Hangzhou City College of Life and Environmental Science Hangzhou Normal University Hangzhou China

LeSPL-CNR is a crucial transcription factor for fruit ripening of Solanum lycopersicum. The cnr (colorless non-ripening) epimutation resulted from hypermethylation in a 286 bp region of LeSPL-CNR promoter inhibits normal fruit ripening. In present study, potential regulators of LeSPL-CNR, which could bind to the specific 286 bp region, were screened via south-western blotting and yeast one-hybrid (Y1H) library screening system. Results indicated that a total of 13 and 19 candidate proteins were acquired respectively, and both ribulose-1,5-bisphosphate carboxylase/oxygenase and 40S ribosomal protein were identified by two methods. These would provide some information for revealing roles of DNA methylation and the regulatory mechanism for LeSPL-CNR.

关键词： Solanum lycopersicum LeSPL-CNR 286 bp Region south-western Blotting Yeast One-Hybrid

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Estimation of Residence Times and Recharge Area of Groundwater in the Moulares Mining Basin by Using Carbon and Oxygen Isotopes (south western Tunisia)

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Journal of Environmental Protection 2010年第4期1卷 466-474页

作者： Younes Hamed Moncef Zairi Wassim Ali Hamed Ben Dhia 不详

Radiogenic carbon (14C) of the DIC (Dissolved Inorganic Carbon) and oxygen-18 were used to understand the hydro- dynamic functioning of the multilayer aquifer system in the Moulares mining basin, southern Tunisia. The results of this study permits identify two groundwater types. A- an old paleoclimatic groundwater, marked by low carbon-14 (14C) activities. B - a recent groundwater, was distinguished by relatively high carbon-14 activities. In addition to these two water types, other groundwater, indicating a mixing effect, is resulting presumably from upward movement from the deeper groundwater. Based on 14C activity and the piston flow type theory the groundwater residence time varies from 5 Kyear to 35 Kyear. Carbon-14 activity and oxygen-18 in Groundwater are active since Mio-Plio-Quaternary and Upper Cretaceous aquifers lead to the identification of paleorecharged water probably during Late Pleistocene and Early to Middle Holocene. The water feedings of these aquifers are mainly provided by infiltration of precipitations, infiltration of irrigation water, lateral feeding from cretaceous relieves from the south and the North and along recent and fossil drainage networks that constitute major fresh water sources in groundwater tables.

关键词： Carbon and Oxygen Isotopes Residence Time Paleo-Recharge south-western Tunisia

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