Role of miR-124 in the regulation of retinoic acid-induced Neuro-2A cell differentiation

作     者：Qun You Qiang Gong Yu-Qiao Han Rou Pi Yi-Jie Du Su-Zhen Dong Qun You;Qiang Gong;Yu-Qiao Han;Rou Pi;Yi-Jie Du;Su-Zhen Dong

作者机构：Shanghai Engineering Research Center of Molecular Therapeutics and New Drug DevelopmentSchool of Chemistry and Molecular EngineeringEast China Normal UniversityShanghaiChina Department of Integrative MedicineHuashan HospitalFudan UniversityShanghaiChina Institutes of Integrative MedicineFudan UniversityShanghaiChina 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2020年第15卷第6期

页      面：1133-1139页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

基　　金：supported by the Natural Science Foundation of Shanghai of China No.16ZR1410500(to SZD) 

主　　题：immunofluorescence MAP2 micro RNA mi R-124 Neuro-2A cells neurite outgrowth neuronal differentiation overexpression real-time PCR retinoic acid 

摘      要：Retinoic acid can cause many types of cells,including mouse neuroblastoma Neuro-2 A cells,to differentiate into neurons.However,it is still unknown whether microRNAs(miRNAs)play a role in this neuronal differentiation.To address this issue,real-time polymerase chain reaction assays were used to detect the expression of several differentiation-related miRNAs during the differentiation of retinoic acid-treated Neuro-2 A cells.The results revealed that miR-124 and miR-9 were upregulated,while miR-125 b was downregulated in retinoic acid-treated Neuro-2 A cells.To identify the miRNA that may play a key role,miR-124 expression was regulated by transfection of miRNA mimics or inhibitors.Morphological analysis results showed that inhibition of miR-124 expression reversed the effects of retinoic acid on neurite outgrowth.Moreover,miR-124 overexpression alone caused Neuro-2 A cells to differentiate into neurons,and its inhibitor could block this effect.These results suggest that miR-124 plays an important role in retinoic acid-induced differentiation of Neuro-2 A cells.