Effect of L-carnitine supplementation during in vitro maturation and in vitro culture on oocyte quality and embryonic development rate of bovines
作者机构：One Health and Veterinary Innovative Research and Development GroupSchool of Veterinary MedicineUniversity of Antioquia050034 MedellinColombia Faculty of Agricultural SciencesSchool of ZootechnicsUniversidad de SucreColombia Genomics and Bioinformatics LabDepartment of Biological SciencesUniversity of the Republic of UruguaySalto CampusUruguay Department of Veterinary Physiology and PharmacologyCollege of Veterinary MedicineTexas A&M UniversityCollege StationTexas 77843-4466USA Grupo de investigación Biotecnología AnimalFacultad de CienciasUniversidad Nacional de ColombiaSede MedellínMedellínColombia
出 版 物：《Asian pacific Journal of Reproduction》 (亚太生殖杂志（英文版）)
年 卷 期：2019年第8卷第6期
页 面：289-296页
学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 1002[医学-临床医学] 0905[农学-畜牧学] 0906[农学-兽医学] 0901[农学-作物学] 0902[农学-园艺学] 100602[医学-中西医结合临床] 10[医学]
基 金：This study was funded by the Administrative Department of Science Technology and Innovation(COLCIENCIAS)(Grant No.727 2015)
主 题：Antioxidants Culture supplement Embryo development Lipid droplets Maturation Mitochondria
摘 要：Objective:To assess the effect of L-carnitine supplementation during in vitro oocyte maturation and in vitro culture process of bovine ***:L-carnitine(3.8 mM)was added to maturation medium and the effect was assessed in the quality(Experiment 1)and in the cleavage and 4-cells stage(Experiment 2).Besides,the effect of L-carnitine addition on maturation medium(3.8 mM)and culture medium(1.5 mM)on embryo rate production was *** Experiment 1,bovine oocytes from abattoir were randomly separated into two groups(the control group and L-carnitine group)forin vitro *** oocytes were examined for cumulus cells expansion as an indicator of maturation,and the content of the mitochondrial activity,the presence of lipid droplets,the reduced glutathione,and the reactive oxygen species were measured by using specific *** Experiment 2,oocytes were matured as performed in Experiment 1,afterward fertilized and cultured until day 3,and cleavage rate and 4-cells stage rate were *** Experiment 3,in vitro maturation and fertilization were done as performed in Experiment 2,but at day 3 of culture,each group of embryos was separated into two new groups,and L-carnitine(1.5 mM)was added in culture media until day *** cleavage and embryo development rate were determined on the basis with the oocytes put on *** rate was calculated from cleaved ***:The cumulus expansion rate at gradeⅢand mitochondrial activity were significantly higher in the L-carnitine group in comparison with the control group(P0.05).In addition,cleavage and the proportion of embryo development and hatching rate were similar for all groups(P0.05).Conclusions:L-carnitine as a supplement in culture media improves the cumulus expansion and increases the mitochondrial activity during in vitro maturation process but has no apparent effect on the cleavage and development of bovine *** investigations of L-carnitine addition on in vit
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